ID C5MLM0_9INFA Unreviewed; 759 AA. AC C5MLM0; DT 28-JUL-2009, integrated into UniProtKB/TrEMBL. DT 28-JUL-2009, sequence version 1. DT 17-JUN-2020, entry version 55. DE RecName: Full=Polymerase basic protein 2 {ECO:0000256|HAMAP-Rule:MF_04062, ECO:0000256|RuleBase:RU361246, ECO:0000256|SAAS:SAAS00943514}; DE AltName: Full=RNA-directed RNA polymerase subunit P3 {ECO:0000256|HAMAP-Rule:MF_04062}; GN Name=PB2 {ECO:0000256|HAMAP-Rule:MF_04062, GN ECO:0000256|RuleBase:RU361246, ECO:0000313|EMBL:ACR49147.1}; OS Influenza A virus (A/chicken/East Java/UT6021/2006(H5N1)). OC Viruses; Riboviria; Negarnaviricota; Polyploviricotina; Insthoviricetes; OC Articulavirales; Orthomyxoviridae; Alphainfluenzavirus. OX NCBI_TaxID=644250 {ECO:0000313|EMBL:ACR49147.1, ECO:0000313|Proteomes:UP000173010}; RN [1] {ECO:0000313|EMBL:ACR49147.1, ECO:0000313|Proteomes:UP000173010} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=A/chicken/East Java/UT6021/2006 {ECO:0000313|EMBL:ACR49147.1}; RX PubMed=19464724; DOI=10.1016/j.virol.2009.04.024; RA Takano R., Nidom C.A., Kiso M., Muramoto Y., Yamada S., Sakai-Tagawa Y., RA Macken C., Kawaoka Y.; RT "Phylogenetic characterization of H5N1 avian influenza viruses isolated in RT Indonesia from 2003-2007."; RL Virology 390:13-21(2009). CC -!- FUNCTION: Plays an essential role in transcription initiation and cap- CC stealing mechanism, in which cellular capped pre-mRNAs are used to CC generate primers for viral transcription. Recognizes and binds the 7- CC methylguanosine-containing cap of the target pre-RNA which is CC subsequently cleaved after 10-13 nucleotides by the viral protein PA. CC Plays a role in the initiation of the viral genome replication and CC modulates the activity of the ribonucleoprotein (RNP) complex. CC {ECO:0000256|HAMAP-Rule:MF_04062, ECO:0000256|SAAS:SAAS01041023}. CC -!- SUBUNIT: Influenza RNA polymerase is composed of three subunits: PB1, CC PB2 and PA. Interacts (via N-terminus) with PB1 (via C-terminus). CC Interacts with nucleoprotein NP (via N-terminus). {ECO:0000256|HAMAP- CC Rule:MF_04062, ECO:0000256|SAAS:SAAS00943505}. CC -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000256|HAMAP-Rule:MF_04062, CC ECO:0000256|RuleBase:RU361246, ECO:0000256|SAAS:SAAS00943502}. Virion CC {ECO:0000256|HAMAP-Rule:MF_04062, ECO:0000256|RuleBase:RU361246}. CC -!- SIMILARITY: Belongs to the influenza viruses PB2 family. CC {ECO:0000256|HAMAP-Rule:MF_04062, ECO:0000256|RuleBase:RU361246, CC ECO:0000256|SAAS:SAAS00943527}. CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of CC feature annotation. {ECO:0000256|HAMAP-Rule:MF_04062}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; GQ122508; ACR49147.1; -; Viral_cRNA. DR Proteomes; UP000173010; Genome. DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0019012; C:virion; IEA:UniProtKB-SubCell. DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule. DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule. DR GO; GO:0006370; P:7-methylguanosine mRNA capping; IEA:UniProtKB-UniRule. DR GO; GO:0075526; P:cap snatching; IEA:UniProtKB-UniRule. DR GO; GO:0039523; P:suppression by virus of host RNA polymerase II activity; IEA:UniProtKB-UniRule. DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule. DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro. DR Gene3D; 3.30.30.90; -; 1. DR HAMAP; MF_04062; INV_PB2; 1. DR InterPro; IPR001591; INV_PB2. DR InterPro; IPR037258; PDB2_C. DR Pfam; PF00604; Flu_PB2; 1. DR SUPFAM; SSF160453; SSF160453; 1. PE 3: Inferred from homology; KW Cap snatching {ECO:0000256|HAMAP-Rule:MF_04062, KW ECO:0000256|SAAS:SAAS00943529}; KW Eukaryotic host gene expression shutoff by virus {ECO:0000256|HAMAP- KW Rule:MF_04062, ECO:0000256|SAAS:SAAS00943497}; KW Eukaryotic host transcription shutoff by virus {ECO:0000256|HAMAP- KW Rule:MF_04062, ECO:0000256|SAAS:SAAS00943528}; KW Host gene expression shutoff by virus {ECO:0000256|HAMAP-Rule:MF_04062, KW ECO:0000256|SAAS:SAAS00943501}; KW Host nucleus {ECO:0000256|HAMAP-Rule:MF_04062, KW ECO:0000256|RuleBase:RU361246, ECO:0000256|SAAS:SAAS00943496}; KW Host-virus interaction {ECO:0000256|HAMAP-Rule:MF_04062, KW ECO:0000256|SAAS:SAAS00943506}; KW Inhibition of host RNA polymerase II by virus {ECO:0000256|HAMAP- KW Rule:MF_04062, ECO:0000256|SAAS:SAAS00943508}; KW mRNA capping {ECO:0000256|HAMAP-Rule:MF_04062, KW ECO:0000256|RuleBase:RU361246, ECO:0000256|SAAS:SAAS00943498}; KW mRNA processing {ECO:0000256|HAMAP-Rule:MF_04062, KW ECO:0000256|RuleBase:RU361246, ECO:0000256|SAAS:SAAS00943500}; KW Viral transcription {ECO:0000256|HAMAP-Rule:MF_04062, KW ECO:0000256|SAAS:SAAS00943507}; KW Virion {ECO:0000256|HAMAP-Rule:MF_04062, ECO:0000256|RuleBase:RU361246, KW ECO:0000256|SAAS:SAAS00943495}. FT MOTIF 736..739 FT /note="Nuclear localization signal" FT /evidence="ECO:0000256|HAMAP-Rule:MF_04062" FT SITE 627 FT /note="Avian adaptation" FT /evidence="ECO:0000256|HAMAP-Rule:MF_04062" SQ SEQUENCE 759 AA; 85821 MW; 1F22DADCD854F33A CRC64; MERIKELRDL MSQSRTREIL TKTTVDHMAI IKKYTSGRQE KNPALRMKWM MAMKYPITAD KRIIEMIPER NEQGQTLWSK TNDAGSDRVM VSPLAVTWWN RNGPTTSAVH YPKVYKTYFE KAERLKHGTF GPVHFRNQVK IRRRVDINPG HADLSAKEAQ DVIMEVVFPN EVGARILTSE SQLTITKEKK EELQDCKIAP LMVAYMLERE LVRKTRFLPV AGGTSSVYIE VLHLTQGTCW EQMYTPGGEV RNDDVDQSLI IAARNIVRRA TVSADPLASL LEMCHSTRIG GTRMVDILRQ NPTEEQAVDI CKAAMGLRIS SSFSFGGFTF KRTSGSSVTK EEEVLTGNLQ TLKIRVHEGY EEFTMVGRRA TAILRKATRR LIQLIVSGRD EQSIAEAIIV AMVFSQEDCM IKAVRGDLNF VNRANQRLNP MHQLLRHFQK DAKVLFQNWG IEPIDNVMGM IGILPDMTPS TEMSLRGVRV SKMGVDEYSS TERVVVSIDR FLRVRDQRGN VLLSPEEVSE TQGTERLTIT YSSSMMWEIN GPESVLVNTY QWIIRNWETV KIQWSQDPTM LYNKMEFEPF QSLVPKAARG QYSGFVRTLF QQMRDVLGTF DTVQIIKLLP FAAAPPEQSR MQFSSLTVNV RGSGMRILVR GNSPVFNYNK ATKRLTVLGK DAGALTEDPD EGTAGVESAV LRGFLILGKE DKRYGPALSI NELSNLAKGE KANVLIGQGD VVLVMKRKRD SSILTDSQTA TKRIRMAIN //