ID C5MLM0_9INFA Unreviewed; 759 AA. AC C5MLM0; DT 28-JUL-2009, integrated into UniProtKB/TrEMBL. DT 28-JUL-2009, sequence version 1. DT 29-APR-2015, entry version 24. DE RecName: Full=Polymerase basic protein 2 {ECO:0000256|RuleBase:RU361246, ECO:0000256|SAAS:SAAS00118352}; GN Name=PB2 {ECO:0000256|RuleBase:RU361246, ECO:0000313|EMBL:ACR49147.1}; OS Influenza A virus (A/chicken/East Java/UT6021/2006(H5N1)). OC Viruses; ssRNA negative-strand viruses; Orthomyxoviridae; OC Influenzavirus A. OX NCBI_TaxID=644250 {ECO:0000313|EMBL:ACR49147.1}; RN [1] {ECO:0000313|EMBL:ACR49147.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=A/chicken/East Java/UT6021/2006 {ECO:0000313|EMBL:ACR49147.1}; RX PubMed=19464724; DOI=10.1016/j.virol.2009.04.024; RA Takano R., Nidom C.A., Kiso M., Muramoto Y., Yamada S., RA Sakai-Tagawa Y., Macken C., Kawaoka Y.; RT "Phylogenetic characterization of H5N1 avian influenza viruses RT isolated in Indonesia from 2003-2007."; RL Virology 390:13-21(2009). RN [2] {ECO:0000313|EMBL:ACR49147.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=A/chicken/East Java/UT6021/2006 {ECO:0000313|EMBL:ACR49147.1}; RA Tankano R.; RL Submitted (MAY-2009) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Plays an essential role in transcription initiation and CC cap-stealing mechanism, in which cellular capped pre-mRNAs are CC used to generate primers for viral transcription. Binds the cap of CC the target pre-RNA which is subsequently cleaved after 10-13 CC nucleotides by PA. Plays a role in the initiation of the viral CC genome replication and modulates the activity of the CC ribonucleoprotein (RNP) complex. In addition, participates in the CC inhibition of type I interferon induction through interaction with CC the host mitochondrial antiviral signaling protein MAVS. CC {ECO:0000256|SAAS:SAAS00161294}. CC -!- SUBUNIT: Influenza RNA polymerase is composed of three subunits: CC PB1, PB2 and PA. Interacts (via N-terminus) with PB1 (via C- CC terminus). Interacts with nucleoprotein NP (via N-terminus). CC Interacts (via N-terminus) with host MAVS (via N-terminus); this CC interaction inhibits host innate immune response. CC {ECO:0000256|SAAS:SAAS00161291}. CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000256|RuleBase:RU361246}. Host CC nucleus {ECO:0000256|RuleBase:RU361246}. Host mitochondrion CC {ECO:0000256|RuleBase:RU361246}. CC -!- SIMILARITY: Belongs to the influenza viruses PB2 family. CC {ECO:0000256|RuleBase:RU361246}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; GQ122508; ACR49147.1; -; Viral_cRNA. DR GO; GO:0033650; C:host cell mitochondrion; IEA:UniProtKB-SubCell. DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0019012; C:virion; IEA:UniProtKB-SubCell. DR GO; GO:0003723; F:RNA binding; IEA:InterPro. DR GO; GO:0003968; F:RNA-directed RNA polymerase activity; IEA:InterPro. DR GO; GO:0006370; P:7-methylguanosine mRNA capping; IEA:UniProtKB-KW. DR GO; GO:0075526; P:cap snatching; IEA:UniProtKB-KW. DR GO; GO:0039545; P:suppression by virus of host MAVS activity; IEA:UniProtKB-KW. DR GO; GO:0039523; P:suppression by virus of host RNA polymerase II activity; IEA:UniProtKB-KW. DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro. DR InterPro; IPR001591; RNA_pol_PB2_orthomyxovir. DR Pfam; PF00604; Flu_PB2; 1. DR ProDom; PD001667; RNA_pol_PB2_orthomyxovir; 1. PE 3: Inferred from homology; KW Cap snatching {ECO:0000256|SAAS:SAAS00118359}; KW Eukaryotic host gene expression shutoff by virus KW {ECO:0000256|SAAS:SAAS00118356}; KW Eukaryotic host transcription shutoff by virus KW {ECO:0000256|SAAS:SAAS00118362}; KW Host gene expression shutoff by virus {ECO:0000256|SAAS:SAAS00118346}; KW Host mitochondrion {ECO:0000256|RuleBase:RU361246}; KW Host nucleus {ECO:0000256|RuleBase:RU361246}; KW Host-virus interaction {ECO:0000256|SAAS:SAAS00118344}; KW Inhibition of host innate immune response by virus KW {ECO:0000256|SAAS:SAAS00161293}; KW Inhibition of host MAVS by virus {ECO:0000256|SAAS:SAAS00161290}; KW Inhibition of host RLR pathway by virus KW {ECO:0000256|SAAS:SAAS00161288}; KW Inhibition of host RNA polymerase II by virus KW {ECO:0000256|SAAS:SAAS00118350}; KW mRNA capping {ECO:0000256|RuleBase:RU361246, KW ECO:0000256|SAAS:SAAS00118427}; KW mRNA processing {ECO:0000256|RuleBase:RU361246, KW ECO:0000256|SAAS:SAAS00118361}; KW Viral immunoevasion {ECO:0000256|SAAS:SAAS00161293}; KW Viral transcription {ECO:0000256|SAAS:SAAS00161279}; KW Virion {ECO:0000256|RuleBase:RU361246}. SQ SEQUENCE 759 AA; 85821 MW; 1F22DADCD854F33A CRC64; MERIKELRDL MSQSRTREIL TKTTVDHMAI IKKYTSGRQE KNPALRMKWM MAMKYPITAD KRIIEMIPER NEQGQTLWSK TNDAGSDRVM VSPLAVTWWN RNGPTTSAVH YPKVYKTYFE KAERLKHGTF GPVHFRNQVK IRRRVDINPG HADLSAKEAQ DVIMEVVFPN EVGARILTSE SQLTITKEKK EELQDCKIAP LMVAYMLERE LVRKTRFLPV AGGTSSVYIE VLHLTQGTCW EQMYTPGGEV RNDDVDQSLI IAARNIVRRA TVSADPLASL LEMCHSTRIG GTRMVDILRQ NPTEEQAVDI CKAAMGLRIS SSFSFGGFTF KRTSGSSVTK EEEVLTGNLQ TLKIRVHEGY EEFTMVGRRA TAILRKATRR LIQLIVSGRD EQSIAEAIIV AMVFSQEDCM IKAVRGDLNF VNRANQRLNP MHQLLRHFQK DAKVLFQNWG IEPIDNVMGM IGILPDMTPS TEMSLRGVRV SKMGVDEYSS TERVVVSIDR FLRVRDQRGN VLLSPEEVSE TQGTERLTIT YSSSMMWEIN GPESVLVNTY QWIIRNWETV KIQWSQDPTM LYNKMEFEPF QSLVPKAARG QYSGFVRTLF QQMRDVLGTF DTVQIIKLLP FAAAPPEQSR MQFSSLTVNV RGSGMRILVR GNSPVFNYNK ATKRLTVLGK DAGALTEDPD EGTAGVESAV LRGFLILGKE DKRYGPALSI NELSNLAKGE KANVLIGQGD VVLVMKRKRD SSILTDSQTA TKRIRMAIN //