ID C3U5A5_9PICO Unreviewed; 2318 AA. AC C3U5A5; DT 16-JUN-2009, integrated into UniProtKB/TrEMBL. DT 16-JUN-2009, sequence version 1. DT 10-MAY-2017, entry version 59. DE RecName: Full=Genome polyprotein {ECO:0000256|SAAS:SAAS00711595}; DE Flags: Fragment; GN Name=gp1 {ECO:0000313|EMBL:ACO92355.1}; OS Saffold virus. OC Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; OC Picornavirales; Picornaviridae; Cardiovirus. OX NCBI_TaxID=434309 {ECO:0000313|EMBL:ACO92355.1}; RN [1] {ECO:0000313|EMBL:ACO92355.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Pak5152 {ECO:0000313|EMBL:ACO92355.1}; RX PubMed=19193786; DOI=10.1128/JVI.02085-08; RA Blinkova O., Kapoor A., Victoria J., Jones M., Wolfe N., Naeem A., RA Shaukat S., Sharif S., Alam M.M., Angez M., Zaidi S., Delwart E.L.; RT "Cardioviruses are genetically diverse and cause common enteric RT infections in South Asian children."; RL J. Virol. 83:4631-4641(2009). CC -!- CATALYTIC ACTIVITY: NTP + H(2)O = NDP + phosphate. CC {ECO:0000256|SAAS:SAAS00817576}. CC -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate CC + RNA(n+1). {ECO:0000256|SAAS:SAAS00383097}. CC -!- CATALYTIC ACTIVITY: Selective cleavage of Gln-|-Gly bond in the CC poliovirus polyprotein. In other picornavirus reactions Glu may be CC substituted for Gln, and Ser or Thr for Gly. CC {ECO:0000256|SAAS:SAAS00711755}. CC -!- SUBCELLULAR LOCATION: Host cytoplasmic vesicle membrane CC {ECO:0000256|SAAS:SAAS00711512}; Peripheral membrane protein CC {ECO:0000256|SAAS:SAAS00711512}; Cytoplasmic side CC {ECO:0000256|SAAS:SAAS00711512}. CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000256|SAAS:SAAS00803776}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; FJ463616; ACO92355.1; -; Genomic_RNA. DR ProteinModelPortal; C3U5A5; -. DR GO; GO:0044162; C:host cell cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell. DR GO; GO:0019030; C:icosahedral viral capsid; IEA:InterPro. DR GO; GO:0044385; C:integral to membrane of host cell; IEA:UniProtKB-KW. DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro. DR GO; GO:0005216; F:ion channel activity; IEA:UniProtKB-KW. DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW. DR GO; GO:0003724; F:RNA helicase activity; IEA:InterPro. DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW. DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro. DR GO; GO:0039707; P:pore formation by virus in membrane of host cell; IEA:UniProtKB-KW. DR GO; GO:0051259; P:protein oligomerization; IEA:UniProtKB-KW. DR GO; GO:0018144; P:RNA-protein covalent cross-linking; IEA:InterPro. DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro. DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW. DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro. DR GO; GO:0019062; P:virion attachment to host cell; IEA:UniProtKB-KW. DR CDD; cd00205; rhv_like; 3. DR Gene3D; 4.10.90.10; -; 1. DR InterPro; IPR015031; Capsid_VP4_Picornavir. DR InterPro; IPR004004; Helic/Pol/Pept_Calicivir-typ. DR InterPro; IPR000605; Helicase_SF3_ssDNA/RNA_vir. DR InterPro; IPR014759; Helicase_SF3_ssRNA_vir. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR000199; Peptidase_C3A/C3B_picornavir. DR InterPro; IPR009003; Peptidase_S1_PA. DR InterPro; IPR002527; Pico_P2B. DR InterPro; IPR001676; Picornavirus_capsid. DR InterPro; IPR033703; Rhv-like. DR InterPro; IPR001205; RNA-dir_pol_C. DR InterPro; IPR007094; RNA-dir_pol_PSvirus. DR Pfam; PF00548; Peptidase_C3; 1. DR Pfam; PF00680; RdRP_1; 1. DR Pfam; PF00073; Rhv; 2. DR Pfam; PF00910; RNA_helicase; 1. DR Pfam; PF08935; VP4_2; 1. DR PRINTS; PR00918; CALICVIRUSNS. DR ProDom; PD649346; Pico_P2B; 1. DR SUPFAM; SSF50494; SSF50494; 1. DR SUPFAM; SSF52540; SSF52540; 1. DR PROSITE; PS50507; RDRP_SSRNA_POS; 1. DR PROSITE; PS51218; SF3_HELICASE_2; 1. PE 4: Predicted; KW ATP-binding {ECO:0000256|SAAS:SAAS00510945}; KW Capsid protein {ECO:0000256|SAAS:SAAS00803779}; KW Coiled coil {ECO:0000256|SAM:Coils}; KW Helicase {ECO:0000256|SAAS:SAAS00124676}; KW Host cytoplasm {ECO:0000256|SAAS:SAAS00711571}; KW Host cytoplasmic vesicle {ECO:0000256|SAAS:SAAS00711589}; KW Host membrane {ECO:0000256|SAAS:SAAS00795395}; KW Host-virus interaction {ECO:0000256|SAAS:SAAS00711757}; KW Hydrolase {ECO:0000256|SAAS:SAAS00510997}; KW Ion channel {ECO:0000256|SAAS:SAAS00711528}; KW Ion transport {ECO:0000256|SAAS:SAAS00711758}; KW Membrane {ECO:0000256|SAAS:SAAS00795440}; KW Nucleotide-binding {ECO:0000256|SAAS:SAAS00510884}; KW Nucleotidyltransferase {ECO:0000256|SAAS:SAAS00510590}; KW Protease {ECO:0000256|SAAS:SAAS00503040}; KW RNA-binding {ECO:0000256|SAAS:SAAS00711776}; KW RNA-directed RNA polymerase {ECO:0000256|SAAS:SAAS00510600}; KW Thiol protease {ECO:0000256|SAAS:SAAS00503128}; KW Transferase {ECO:0000256|SAAS:SAAS00510371}; KW Transport {ECO:0000256|SAAS:SAAS00711554}; KW Viral attachment to host cell {ECO:0000256|SAAS:SAAS00711583}; KW Viral ion channel {ECO:0000256|SAAS:SAAS00711569}; KW Viral RNA replication {ECO:0000256|SAAS:SAAS00660848}; KW Virion {ECO:0000256|SAAS:SAAS00803780}; KW Virus entry into host cell {ECO:0000256|SAAS:SAAS00711553}. FT DOMAIN 1310 1475 SF3 helicase. {ECO:0000259|PROSITE: FT PS51218}. FT DOMAIN 2095 2213 RdRp catalytic. {ECO:0000259|PROSITE: FT PS50507}. FT COILED 1553 1580 {ECO:0000256|SAM:Coils}. FT NON_TER 2318 2318 {ECO:0000313|EMBL:ACO92355.1}. SQ SEQUENCE 2318 AA; 259535 MW; 639293B2F85610EC CRC64; MCFTPSLWFK NCHSFSKPSG LGFLILLTGN MACKHGYPFL CPLCTAIDVS SDGSFALLFD NEWYPTDLLT VDLDDDVFYP LDCLMDWTDL PLIQDVLMEP QGNSNSSDKN NSQSSGNEGV IINNYYSNQY QNSIDLSANA NGVGKENSKP QGQLMNILGS AADAFKNIAP LLMDQDTEEM TNLSDRVASD TAGNTATNTQ STVGRLFGYA SRHRGKHPTS CADRATDKVL AAERYYTIKL ATWDTTLKAF DHIRVPFPHI LSGKEGGVFG ATLRRHYLCK CGWRIQVQCN ASQFHAGSLL IFMAPEFDTS PYESETEPDK NKVFTMDTTW QSGTKCLRGH SFESKTNQSL RPLSLNHQNT WQWTIYPHQI LNLRTNTTCD IEVPYVNICP TSSWTQHANW TLVVAVLTPL QCPTGSAPDV EITASIQPVK PVFNGLRHSV LEPDSPFPGT IREHAGTFYS TTPDTTVPVY GKTISTPANY MCGEFTDLLS LCKIPTFIGN NLDSKWNPYF SSTNSVNENV SLVTYQVTLS SINLANTMLS SVARNFNQYR GSLNFLFVFT GSAMVKGKFL ISYTPPGADK PKNRNQAMQA TYAIWDLGLN SSYNFTVPFI SPTHFRQTSY TTSTITSVDG WISVWQLTPL TYPANTPTHS DILTLVSGGD DFTLRMPVTP TKYVPHGVDN AEKGKVADDN ASTDFVAEPV KLPENQTEVG FFYDRAVQAC VLKSSQNFSD TFQLQSSDTL LNQTILTPLP NYKVDQSKEE LVEQYRWLES AGTSTQPPYK TKQDWNFVMF SPFTYYKCDL EVTLTALNNN GQGALVRYSP CGAPADITTQ TMSSTPSLAD TRDPHMWFVG PGTTNQVSFV LPFTSPLSVL PAVWFNGYAN FDNSSRYGVA PNADFGRLFV QGSGTFSVHF RYKKMRVFCP RPTVYFPWPS NPQRTKIHAE NPVPILELQN PISIYRVDLF INFSDEIIQF TYKVHGRTVC QYEIPGFGLS RSGRLLVCMG EKPCQLPIST PKCFYHIVFT GSRNSFGVSI YKARYRPWKQ PLHDELFDYG FSTFTDFFKA VRDYHASYYK QRLQHDIETN PGPVQSVFQL QGGVLTKSQA PMSGLQSMLL RAIGIEADCT EFTRAVNLIT DLCNTWESAK TTLSSPEFWT KMVMRIVKMV AASVLYLHNP DLTTTVCLSL MAGIDILTND SVFNWLSSKL SKFFHTPAPP IVPLLQQQSP IREANDNFNL AKNIEWAIKT IKRIVEWITS WFKQEETSPQ AKLDKMLADF PEHCASILAM RNGRKAYTDC AGAFKYFEQL YNLAVQCKRI PLATLCEKFK NKHDHAVARP EPVVVVLRGN AGQGKSVTSQ IIAQAVSKLS FGRQSVYSLP PDSDYLDGYE NQYSVIMDDL GQNPDGEDFK VFCQMVSSTN FLPNMAHLEK KGTPFTSNFI IATTNLPKFR PVTVAHYPAV DRRITFDLTV EAGDECVTHN GMLDVEKAFE EIPGKPQLDC FNTDCRLLHK RGVRFVCNRT KNVYNLQQVV KMVKNTIDNK VENLKKMNTL VAQSPGNDMD YVLTCLRQTN AALQDQIDEL QEAFNQAQER QNFLSDWLKV SAIVFASIAS LSAVCKLVSR FKNLVCPAPV QIQLSEGEQA AYSGGKKGEK QTLQVLDVQG GGKIVAQAGN PVMDYEVNIA KNMVTPITFF YADKAQVTQS CLLIKGHLFV VNRHVAETDW CAFELKGTRH ERDSVQMRSV NKSGMEVDLT FVKVVKGPLF KDNSKKFCSK DDDFPARNET VIGIMNTGVP FVFTGKFLIG NQPVNTTTGA CFNHCIHYRA TTHRGWGGSA LICHVNGKKA VYAMHSAGGG GIAAATIITQ EMIEAAEKAL DCLTPQGAIV EIGIDTVVHV PRKTKLRRTV AHPCFQPKFE PAVLSRYDPR TTKDVDQVAF SKHTTNLEEL PPVFTVVAKE YANRVFTTLG KENQILTPEQ AILGLSGMDP MEKDTSPGLP YTQQGLKRAQ LVNFEQGTMA QNLKEAHTKL TKGNYEDILY QSFLKDEIRP IEKIHEAKTR IVDVPPFHHC IWGRQLLGRF ASKFQTNPGL DLGSAIGTDP DTDWTAFAFQ LLQYKYVYDV DYSNFDASHS TAMFEILIEN FFTVENGFDE RIGDYLRSLA VSRHAFEERR VLVRGGLPSG CAATSMLNTI INNIVIRAAL DLTYSNFEFD DIKVLSYGDD LLIATNYQIN FNLVKQRLAP FNYKITPANK TVEFPETSNL YEVTFLKRKF VRHNSCLFKP QMDTENLKAM VSYCRPGTLK EKLNSIALLA VHSGKSVYDE IFDPFRRIGI IVPEHSTM //