ID ASSY_BACAC Reviewed; 401 AA. AC C3L9T6; DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot. DT 16-JUN-2009, sequence version 1. DT 19-FEB-2014, entry version 35. DE RecName: Full=Argininosuccinate synthase; DE EC=6.3.4.5; DE AltName: Full=Citrulline--aspartate ligase; GN Name=argG; OrderedLocusNames=BAMEG_4912; OS Bacillus anthracis (strain CDC 684 / NRRL 3495). OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; OC Bacillus cereus group. OX NCBI_TaxID=568206; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=CDC 684 / NRRL 3495; RA Dodson R.J., Munk A.C., Brettin T., Bruce D., Detter C., Tapia R., RA Han C., Sutton G., Sims D.; RT "Genome sequence of Bacillus anthracis str. CDC 684."; RL Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: ATP + L-citrulline + L-aspartate = AMP + CC diphosphate + N(omega)-(L-arginino)succinate. CC -!- PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis; L- CC arginine from L-ornithine and carbamoyl phosphate: step 2/3. CC -!- SUBUNIT: Homotetramer (By similarity). CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity). CC -!- SIMILARITY: Belongs to the argininosuccinate synthase family. Type CC 1 subfamily. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP001215; ACP12345.1; -; Genomic_DNA. DR RefSeq; YP_002817429.1; NC_012581.1. DR ProteinModelPortal; C3L9T6; -. DR STRING; 568206.BAMEG_4912; -. DR EnsemblBacteria; ACP12345; ACP12345; BAMEG_4912. DR GeneID; 7781994; -. DR KEGG; bah:BAMEG_4912; -. DR PATRIC; 18799661; VBIBacAnt127120_5159. DR eggNOG; COG0137; -. DR HOGENOM; HOG000230093; -. DR KO; K01940; -. DR OMA; IYNGYWW; -. DR OrthoDB; EOG6K9QCV; -. DR ProtClustDB; PRK00509; -. DR BioCyc; BANT568206:GHVT-4849-MONOMER; -. DR UniPathway; UPA00068; UER00113. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0004055; F:argininosuccinate synthase activity; IEA:UniProtKB-HAMAP. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-HAMAP. DR GO; GO:0006526; P:arginine biosynthetic process; IEA:UniProtKB-HAMAP. DR Gene3D; 3.40.50.620; -; 1. DR Gene3D; 3.90.1260.10; -; 1. DR HAMAP; MF_00005; Arg_succ_synth_type1; 1. DR InterPro; IPR001518; Arginosuc_synth. DR InterPro; IPR018223; Arginosuc_synth_CS. DR InterPro; IPR023434; Arginosuc_synth_type_1_subfam. DR InterPro; IPR024074; AS_cat/multimer_dom_body. DR InterPro; IPR014729; Rossmann-like_a/b/a_fold. DR Pfam; PF00764; Arginosuc_synth; 1. DR TIGRFAMs; TIGR00032; argG; 1. DR PROSITE; PS00564; ARGININOSUCCIN_SYN_1; 1. DR PROSITE; PS00565; ARGININOSUCCIN_SYN_2; 1. PE 3: Inferred from homology; KW Amino-acid biosynthesis; Arginine biosynthesis; ATP-binding; KW Complete proteome; Cytoplasm; Ligase; Nucleotide-binding. FT CHAIN 1 401 Argininosuccinate synthase. FT /FTId=PRO_1000191878. FT NP_BIND 9 17 ATP (By similarity). FT BINDING 86 86 Citrulline (By similarity). FT BINDING 116 116 ATP; via amide nitrogen (By similarity). FT BINDING 118 118 Aspartate (By similarity). FT BINDING 122 122 Aspartate (By similarity). FT BINDING 122 122 Citrulline (By similarity). FT BINDING 123 123 Aspartate (By similarity). FT BINDING 126 126 Citrulline (By similarity). FT BINDING 174 174 Citrulline (By similarity). FT BINDING 183 183 Citrulline (By similarity). FT BINDING 259 259 Citrulline (By similarity). FT BINDING 271 271 Citrulline (By similarity). SQ SEQUENCE 401 AA; 44693 MW; D73DE756DC5AD34E CRC64; MEKKKVVLAY SGGLDTSVAI KWLQEKNYDI IALCLDLGEG KDLAFVKEKA LSVGAIKSYM IDVQEEFANE YALMAMQAHT LYEGKYPLVS ALSRPLIAKK LVEIAEQEGA TAVAHGCTGK GNDQVRFEVS IQALNPYLEV IAPVREWKWS REEEIAYAKE NNVPIPINLD SPFSIDQNLW GRSNECGILE DPWAAPPEDA YEMTLALEDT PNKPEFVEIG FEAGVPTTLN GTAYPLSELI KTLNALAGKH GVGRIDHVEN RLVGIKSREV YECPAAMTLI TAHKELEDLT LVKEVAHFKP MIEQKITELI YNGLWFSPLK QALHAFLQET QKNVTGTVRV KLFKGHAIVE GRKSEYSLYD EKLATYTAQD EFNHDAAVGF ISLFGLPTKV YSQVNQKKVE A //