ID ISPH_BACC3 Reviewed; 316 AA. AC C1ESI2; DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot. DT 26-MAY-2009, sequence version 1. DT 28-JUN-2011, entry version 21. DE RecName: Full=4-hydroxy-3-methylbut-2-enyl diphosphate reductase; DE EC=1.17.1.2; GN Name=ispH; OrderedLocusNames=BCA_4399; OS Bacillus cereus (strain 03BB102). OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus; OC Bacillus cereus group. OX NCBI_TaxID=572264; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=03BB102; RA Dodson R.J., Jackson P., Munk A.C., Brettin T., Bruce D., Detter C., RA Tapia R., Han C., Sutton G., Sims D.; RT "Genome sequence of Bacillus cereus 03BB102."; RL Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate CC into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate CC (DMAPP) (By similarity). CC -!- CATALYTIC ACTIVITY: Isopentenyl diphosphate + NAD(P)(+) + H(2)O = CC (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. CC -!- CATALYTIC ACTIVITY: Dimethylallyl diphosphate + NAD(P)(+) + H(2)O CC = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. CC -!- COFACTOR: Binds 1 3Fe-4S cluster (By similarity). CC -!- PATHWAY: Isoprenoid biosynthesis; dimethylallyl diphosphate CC biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3- CC methylbutenyl diphosphate: step 1/1. CC -!- PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate CC biosynthesis via DXP pathway; isopentenyl diphosphate from 1- CC deoxy-D-xylulose 5-phosphate: step 6/6. CC -!- SIMILARITY: Belongs to the IspH family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP001407; ACO28637.1; -; Genomic_DNA. DR RefSeq; YP_002751647.1; NC_012472.1. DR EnsemblBacteria; EBBACT00000115956; EBBACP00000109883; EBBACG00000113293. DR GeneID; 7690822; -. DR GenomeReviews; CP001407_GR; BCA_4399. DR KEGG; bcx:BCA_4399; -. DR GeneTree; EBGT00050000002965; -. DR OMA; WGAPVYV; -. DR ProtClustDB; PRK01045; -. DR GO; GO:0051538; F:3 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW. DR GO; GO:0051745; F:4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity; IEA:EC. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0019288; P:isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway; IEA:InterPro. DR HAMAP; MF_00191; IspH; 1; -. DR InterPro; IPR003451; LytB. DR Pfam; PF02401; LYTB; 1. DR TIGRFAMs; TIGR00216; IspH_lytB; 1. PE 3: Inferred from homology; KW 3Fe-4S; Complete proteome; Iron; Iron-sulfur; Isoprene biosynthesis; KW Metal-binding; NADP; Oxidoreductase. FT CHAIN 1 316 4-hydroxy-3-methylbut-2-enyl diphosphate FT reductase. FT /FTId=PRO_1000124275. SQ SEQUENCE 316 AA; 34939 MW; 6C2B8D19112976BB CRC64; MKIVKISPRG YCYGVVDAMV IARNAALDTS LPRPIYILGM IVHNKHVTDA FEEDGIITLD GPSRLDILDK IDSGTVIFTA HGVSPEVKQR AKEKGLTTID ATCPDVTKTH DLIEAKKAEG YHVIYIGKKN HPEPEGAVGI APDIVHLIEK ADDLKTLEIP TDKILVTNQT TMSQWDVQHL MEDIQKKFPT AEFHKEICLA TQVRQEAVAK QADVADLTIV VGDPKSNNSN RLAQVSQEIA GTKAYRVADV SEIKLEWLQG VENVAVTAGA STPTPITKEV IAFLEQYDPM NPATWERVRK VPLQKILPRV KVKKEQ //