ID   ILVD_AZOVD              Reviewed;         612 AA.
AC   C1DI94;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   26-MAY-2009, sequence version 1.
DT   22-JUL-2015, entry version 41.
DE   RecName: Full=Dihydroxy-acid dehydratase {ECO:0000255|HAMAP-Rule:MF_00012};
DE            Short=DAD {ECO:0000255|HAMAP-Rule:MF_00012};
DE            EC=4.2.1.9 {ECO:0000255|HAMAP-Rule:MF_00012};
GN   Name=ilvD {ECO:0000255|HAMAP-Rule:MF_00012};
GN   OrderedLocusNames=Avin_03310;
OS   Azotobacter vinelandii (strain DJ / ATCC BAA-1303).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Azotobacter.
OX   NCBI_TaxID=322710;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DJ / ATCC BAA-1303;
RX   PubMed=19429624; DOI=10.1128/JB.00504-09;
RA   Setubal J.C., Dos Santos P., Goldman B.S., Ertesvaag H., Espin G.,
RA   Rubio L.M., Valla S., Almeida N.F., Balasubramanian D., Cromes L.,
RA   Curatti L., Du Z., Godsy E., Goodner B., Hellner-Burris K.,
RA   Hernandez J.A., Houmiel K., Imperial J., Kennedy C., Larson T.J.,
RA   Latreille P., Ligon L.S., Lu J., Maerk M., Miller N.M., Norton S.,
RA   O'Carroll I.P., Paulsen I., Raulfs E.C., Roemer R., Rosser J.,
RA   Segura D., Slater S., Stricklin S.L., Studholme D.J., Sun J.,
RA   Viana C.J., Wallin E., Wang B., Wheeler C., Zhu H., Dean D.R.,
RA   Dixon R., Wood D.;
RT   "Genome sequence of Azotobacter vinelandii, an obligate aerobe
RT   specialized to support diverse anaerobic metabolic processes.";
RL   J. Bacteriol. 191:4534-4545(2009).
CC   -!- CATALYTIC ACTIVITY: 2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-
CC       oxobutanoate + H(2)O. {ECO:0000255|HAMAP-Rule:MF_00012}.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00012};
CC       Note=Binds 1 [4Fe-4S] cluster. {ECO:0000255|HAMAP-Rule:MF_00012};
CC   -!- PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; L-
CC       isoleucine from 2-oxobutanoate: step 3/4. {ECO:0000255|HAMAP-
CC       Rule:MF_00012}.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-valine biosynthesis; L-valine
CC       from pyruvate: step 3/4. {ECO:0000255|HAMAP-Rule:MF_00012}.
CC   -!- SIMILARITY: Belongs to the IlvD/Edd family. {ECO:0000255|HAMAP-
CC       Rule:MF_00012}.
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DR   EMBL; CP001157; ACO76591.1; -; Genomic_DNA.
DR   RefSeq; WP_012699019.1; NC_012560.1.
DR   STRING; 322710.Avin_03310; -.
DR   EnsemblBacteria; ACO76591; ACO76591; Avin_03310.
DR   KEGG; avn:Avin_03310; -.
DR   PATRIC; 21027014; VBIAzoVin72790_0309.
DR   eggNOG; COG0129; -.
DR   HOGENOM; HOG000173155; -.
DR   KO; K01687; -.
DR   OMA; QGRNMAG; -.
DR   OrthoDB; EOG6MSS24; -.
DR   BioCyc; AVIN322710:GJ0M-328-MONOMER; -.
DR   UniPathway; UPA00047; UER00057.
DR   UniPathway; UPA00049; UER00061.
DR   Proteomes; UP000002424; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0004160; F:dihydroxy-acid dehydratase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0009097; P:isoleucine biosynthetic process; IEA:UniProtKB-HAMAP.
DR   GO; GO:0009099; P:valine biosynthetic process; IEA:UniProtKB-HAMAP.
DR   HAMAP; MF_00012; IlvD; 1.
DR   InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR   InterPro; IPR004404; DihydroxyA_deHydtase.
DR   InterPro; IPR000581; DiOHA_6PGluconate_deHydtase.
DR   InterPro; IPR020558; DiOHA_6PGluconate_deHydtase_CS.
DR   PANTHER; PTHR21000; PTHR21000; 1.
DR   Pfam; PF00920; ILVD_EDD; 1.
DR   SUPFAM; SSF52016; SSF52016; 1.
DR   TIGRFAMs; TIGR00110; ilvD; 1.
DR   PROSITE; PS00886; ILVD_EDD_1; 1.
DR   PROSITE; PS00887; ILVD_EDD_2; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Amino-acid biosynthesis;
KW   Branched-chain amino acid biosynthesis; Complete proteome; Iron;
KW   Iron-sulfur; Lyase; Metal-binding; Reference proteome.
FT   CHAIN         1    612       Dihydroxy-acid dehydratase.
FT                                /FTId=PRO_1000201771.
FT   METAL       122    122       Iron-sulfur (4Fe-4S). {ECO:0000255|HAMAP-
FT                                Rule:MF_00012}.
FT   METAL       193    193       Iron-sulfur (4Fe-4S). {ECO:0000255|HAMAP-
FT                                Rule:MF_00012}.
SQ   SEQUENCE   612 AA;  65233 MW;  2EE6681756D75FB1 CRC64;
     MPDYRSKTST FGRNMAGARA LWRATGMKDE DFKKPIIAVA NSFTQFVPGH VHLKDLGQLV
     AREIEKAGGV AKEFDTIAVD DGIAMGHDGM LYSLPSREII ADSVEYMVNA HCADALVCIS
     NCDKITPGML MASLRLNIPV VFVSGGPMEA GKTKLSQHGL DLVDAMVIAA DSSASDEKVE
     AYERSACPTC GSCSGMFTAN SMNCLTEALG LSLPGNGTIV ATHADRRELF LQAGRTIVDL
     CRRYYQEGDE SVLPRAIASR AAFENAMTLD IAMGGSTNTI LHLLAAAQEA EVDFDLHAID
     ALSRQVPQLC KVAPNTPKYH IEDVHRAGGI VAILGELARA GLLNTEVATV HSKTLGEAIE
     KWDVRVNRDQ AVHTFFKAGP AGIPSQEAFS QSERWDSLDL DRAEGCIRDL EHAFSTEGGL
     AVLYGNIALD GCVVKTAGVD ESILVFEGTA KIFESQDAAV KGILGDEVKP GDVVVIRYEG
     PKGGPGMQEM LYPTSYLKSK GLGKQCALLT DGRFSGGTSG LSIGHASPEA AAGGAIGLVE
     DGDKILIDIP NRSINLLVSD EELKARRLHQ DHKGWKPAAP RARKVSTALK AYALLATSAD
     KGAVRNKALL DE
//