ID C1B7Z5_RHOOB Unreviewed; 1114 AA. AC C1B7Z5; DT 26-MAY-2009, integrated into UniProtKB/TrEMBL. DT 26-MAY-2009, sequence version 1. DT 19-JAN-2010, entry version 7. DE RecName: Full=Lysyl-tRNA synthetase; DE EC=6.1.1.6; GN OrderedLocusNames=ROP_35510; OS Rhodococcus opacus (strain B4). OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Corynebacterineae; Nocardiaceae; Rhodococcus. OX NCBI_TaxID=632772; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RA Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S., RA Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S.; RT "Comparison of the complete genome sequences of Rhodococcus RT erythropolis PR4 and Rhodococcus opacus B4."; RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate CC + L-lysyl-tRNA(Lys). CC -!- COFACTOR: Binds 3 magnesium ions per subunit (By similarity). CC -!- SUBUNIT: Homodimer (By similarity). CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity). CC -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase CC family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AP011115; BAH51798.1; -; Genomic_DNA. DR RefSeq; YP_002780743.1; -. DR GeneID; 7746772; -. DR GenomeReviews; AP011115_GR; ROP_35510. DR KEGG; rop:ROP_35510; -. DR OMA; ATRRDKA; -. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0004824; F:lysine-tRNA ligase activity; IEA:EC. DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW. DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro. DR GO; GO:0006430; P:lysyl-tRNA aminoacylation; IEA:InterPro. DR InterPro; IPR004364; aa-tRNA-synt_II. DR InterPro; IPR018150; aa-tRNA-synt_II-like. DR InterPro; IPR006195; aa-tRNA-synth_II_cons-reg. DR InterPro; IPR002313; Lys-tRNA-synth_II. DR InterPro; IPR018149; Lys-tRNA-synth_II_C. DR InterPro; IPR012340; NA-bd_OB-fold. DR InterPro; IPR016027; NA-bd_OB-fold-like. DR InterPro; IPR004365; NA_bd_OB_tRNA-helicase. DR InterPro; IPR016732; UCP018688. DR Gene3D; G3DSA:2.40.50.140; OB_NA_bd_sub; 1. DR PANTHER; PTHR22594; aa-tRNA-synt_II; 1. DR PANTHER; PTHR22594:SF4; tRNA-synt_lys_2; 1. DR Pfam; PF09924; DUF2156; 1. DR Pfam; PF00152; tRNA-synt_2; 1. DR Pfam; PF01336; tRNA_anti; 1. DR PRINTS; PR00982; TRNASYNTHLYS. DR TIGRFAMs; TIGR00499; lysS_bact; 1. DR PROSITE; PS50862; AA_TRNA_LIGASE_II; 1. PE 3: Inferred from homology; KW ATP-binding; Aminoacyl-tRNA synthetase; Complete proteome; Cytoplasm; KW Ligase; Magnesium; Metal-binding; Nucleotide-binding; KW Protein biosynthesis. SQ SEQUENCE 1114 AA; 122292 MW; BAE0618F5814EB62 CRC64; MSASTETHHA SEAAVPTAPR PRPALGSKSG RLHQVPHIAG LILGVFSVLV FLWSISPVLR YYVHVPREYI DTYYFDAPDT SLSWALVVAL LAAALASRKR IAWWLLTIYL VLILITNVIV SITDRNVNAM VAAVVQVVLI GILVAARPEF YTRVRRGAGW KALGVLIVGL AIGTLVGWGL VELFPGTLPQ GERFLWALNR VTALAAADNE QFSGRPHGFV NTLLGLFGAM ALLAAVITLF RAQRSHNALT GNDESALRGL LLQYGADDSL GYFATRRDKA VVFAPSGKAA ITYRVELGVC LASGDPIGDP EAWPHAIEAW QTLASQYGWA TAVMGASETG ATAYNKAGLT VLQLGDEAIL RTREFNLSGR DMRQVRQAVT RVRRQGVTVR IRRHRDVPPE EMAEAIRLAD AWRDTETERG FSMALGRLGD RLDGDCLLVE AIAEDGEIDG ILSLVPWGPT GVSLDLMRRK PTSPNGVVEL MVSELATTSD QFGITKVSLN FAVFRSVFEE GSRIGAGPIL RIWRSILVFF SRWWQLEALY RSNVKYQPEW VPRFLCFDDN RELLRVGFAS AVAEGFVTLP RFGRSGTHDA IEHTGHHAAV PAALVAAEGL HSDGSAPGEG LAPTATGPKR PEQVRVRLDK LTGLAEQGID PYPVAYPPSH TVTEAVESPE GTTVRIAGRL LRIRDYGGVV FAVVRDWSGD IQVLVDEARV GTDRIRAFAA EFDLGDLVEV AGVIGYSRRG ALSLLANEWR MTGKCLHPLP DKWKGLSDPE TRVRQRYVDL AINTDARRLL EARSAVVKSL RDSLGGRGFL EVETPILQQV HGGANAAPFL THINAYNLDL YLRIAPELYL KRLCVAGMEK VFEIGRVFRN EGVDFKHNPE FTILEAYEAH SDYEKMMVLC RELIQTAAVA AYGREIIMRP GPDGTLVEVD ISGEWPVKTM HQAVAEKLGV DVSPETPLAE LQRLCDEHEI PYQSTWDAGA VAQEMYEHLV EDYTEFPTFY TNFPTSMSPL TRPHPTIPGV AAKWDLVAWG VELGTAYSEL TDPVDQRNRL TEQSMLAAGG DEEAMELDED FLQALEHAMP PTGGLGMGVD RVVMLITGGS IRETLAFPLA KPRQ //