ID LAC_PHEIG Reviewed; 8 AA. AC C0HJB2; DT 01-MAY-2013, integrated into UniProtKB/Swiss-Prot. DT 01-MAY-2013, sequence version 1. DT 11-DEC-2019, entry version 8. DE RecName: Full=Laccase {ECO:0000303|Ref.1}; DE EC=1.10.3.2 {ECO:0000269|Ref.1}; DE Flags: Fragment; OS Phellinus igniarius (Willow bracket) (Boletus igniarius). OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Hymenochaetales; Hymenochaetaceae; Phellinus. OX NCBI_TaxID=40472; RN [1] {ECO:0000305} RP PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, RP BIOPHYSICOCHEMICAL PROPERTIES, AND SUBCELLULAR LOCATION. RC STRAIN=MW0801 {ECO:0000269|Ref.1}; TISSUE=Mycelium {ECO:0000269|Ref.1}; RA Sun J., Chen Q.J., Zhu J.M., Zhu X.M., Wang H.X., Zhang G.Q.; RT "An extracellular laccase with antiproliferative activity from the RT medicinal fungi Phellinus igniarius."; RL Submitted (FEB-2013) to UniProtKB. CC -!- FUNCTION: Lignin degradation and detoxification of lignin-derived CC products. Has highest activity for ABTS but can also act on guaiacol, CC catechol and hydroquinone with decreasing activity. CC {ECO:0000269|Ref.1}. CC -!- CATALYTIC ACTIVITY: CC Reaction=4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O; CC Xref=Rhea:RHEA:11276, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, CC ChEBI:CHEBI:17594, ChEBI:CHEBI:17977; EC=1.10.3.2; CC Evidence={ECO:0000269|Ref.1}; CC -!- BIOPHYSICOCHEMICAL PROPERTIES: CC Kinetic parameters: CC KM=1.31 mM for ABTS {ECO:0000269|Ref.1}; CC pH dependence: CC Optimum pH is 2.4. {ECO:0000269|Ref.1}; CC Temperature dependence: CC Optimum temperature is 20 degrees Celsius. {ECO:0000269|Ref.1}; CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space CC {ECO:0000269|Ref.1}. CC -!- MISCELLANEOUS: Inhibits cell proliferation of HepG2 hepatoma cell line CC with an IC(50) of 2.6 uM and L1210 mouse leukemia cell line with an CC IC(50)=3.2 uM respectively. {ECO:0000269|Ref.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell. DR GO; GO:0052716; F:hydroquinone:oxygen oxidoreductase activity; IEA:UniProtKB-EC. DR GO; GO:0046274; P:lignin catabolic process; IEA:UniProtKB-KW. PE 1: Evidence at protein level; KW Direct protein sequencing; Lignin degradation; Oxidoreductase; Secreted. FT CHAIN 1..>8 FT /note="Laccase" FT /id="PRO_0000422126" FT NON_TER 8 FT /evidence="ECO:0000303|Ref.1" SQ SEQUENCE 8 AA; 799 MW; 87CB1AB2D768705D CRC64; AIGPVDEV //