ID LAC_PHEIG Reviewed; 8 AA. AC C0HJB2; DT 01-MAY-2013, integrated into UniProtKB/Swiss-Prot. DT 01-MAY-2013, sequence version 1. DT 01-OCT-2014, entry version 4. DE RecName: Full=Laccase {ECO:0000303|Ref.1}; DE EC=1.10.3.2 {ECO:0000269|Ref.1}; DE Flags: Fragment; OS Phellinus igniarius (Willow bracket) (Boletus igniarius). OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; OC Agaricomycetes; Hymenochaetales; Hymenochaetaceae; Phellinus. OX NCBI_TaxID=40472; RN [1] {ECO:0000305} RP PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, RP BIOPHYSICOCHEMICAL PROPERTIES, AND SUBCELLULAR LOCATION. RC STRAIN=MW0801 {ECO:0000269|Ref.1}; RC TISSUE=Mycelium {ECO:0000269|Ref.1}; RA Sun J., Chen Q.J., Zhu J.M., Zhu X.M., Wang H.X., Zhang G.Q.; RT "An extracellular laccase with antiproliferative activity from the RT medicinal fungi Phellinus igniarius."; RL Submitted (FEB-2013) to UniProtKB. CC -!- FUNCTION: Lignin degradation and detoxification of lignin-derived CC products. Has highest activity for ABTS but can also act on CC guaiacol, catechol and hydroquinone with decreasing activity. CC {ECO:0000269|Ref.1}. CC -!- CATALYTIC ACTIVITY: 4 benzenediol + O(2) = 4 benzosemiquinone + 2 CC H(2)O. {ECO:0000269|Ref.1}. CC -!- BIOPHYSICOCHEMICAL PROPERTIES: CC Kinetic parameters: CC KM=1.31 mM for ABTS {ECO:0000269|Ref.1}; CC pH dependence: CC Optimum pH is 2.4. {ECO:0000269|Ref.1}; CC Temperature dependence: CC Optimum temperature is 20 degrees Celsius. {ECO:0000269|Ref.1}; CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space CC {ECO:0000269|Ref.1}. CC -!- MISCELLANEOUS: Inhibits cell proliferation of HepG2 hepatoma cell CC line with an IC(50) of 2.6 uM and L1210 mouse leukemia cell line CC with an IC(50)=3.2 uM respectively. {ECO:0000269|Ref.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell. DR GO; GO:0052716; F:hydroquinone:oxygen oxidoreductase activity; IEA:UniProtKB-EC. DR GO; GO:0046274; P:lignin catabolic process; IEA:UniProtKB-KW. PE 1: Evidence at protein level; KW Direct protein sequencing; Lignin degradation; Oxidoreductase; KW Secreted. FT CHAIN 1 >8 Laccase. FT /FTId=PRO_0000422126. FT NON_TER 8 8 {ECO:0000303|Ref.1}. SQ SEQUENCE 8 AA; 799 MW; 87CB1AB2D768705D CRC64; AIGPVDEV //