ID LAC_SANBA Reviewed; 8 AA. AC C0HJB2; DT 01-MAY-2013, integrated into UniProtKB/Swiss-Prot. DT 01-MAY-2013, sequence version 1. DT 29-SEP-2021, entry version 11. DE RecName: Full=Laccase {ECO:0000303|Ref.1}; DE EC=1.10.3.2 {ECO:0000269|Ref.1}; DE Flags: Fragment; OS Sanghuangporus baumii (Phellinus baumii). OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Hymenochaetales; Hymenochaetaceae; Sanghuangporus. OX NCBI_TaxID=108892 {ECO:0000269|Ref.1}; RN [1] RP PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ACTIVITY RP REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, AND SUBCELLULAR LOCATION. RC STRAIN=MW0801 {ECO:0000269|Ref.1}; TISSUE=Mycelium {ECO:0000269|Ref.1}; RX DOI=10.1016/j.molcatb.2013.10.004; RA Sun J., Chen Q.J., Zhu J.M., Wang H.X., Zhang G.Q.; RT "An extracellular laccase with antiproliferative activity from the RT sanghuang mushroom Inonotus baumii."; RL J. Mol. Catal., B Enzym. 99:20-25(2014). CC -!- FUNCTION: Lignin degradation and detoxification of lignin-derived CC products. Exhibits broad substrate specificity against a range of CC aromatic compounds with highest activity against guaiacol. Shows lower CC activity against 4-methylcatechol, 4-hydroxyindole, catechol, CC hydroquinone and 2,6-dimethoxy-phenol. {ECO:0000269|Ref.1}. CC -!- CATALYTIC ACTIVITY: CC Reaction=4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O; CC Xref=Rhea:RHEA:11276, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, CC ChEBI:CHEBI:17594, ChEBI:CHEBI:17977; EC=1.10.3.2; CC Evidence={ECO:0000269|Ref.1}; CC -!- COFACTOR: CC Name=Cu(2+); Xref=ChEBI:CHEBI:29036; Evidence={ECO:0000269|Ref.1}; CC Note=Binds 4 Cu cations per monomer. {ECO:0000250|UniProtKB:Q70KY3}; CC -!- ACTIVITY REGULATION: Inhibited by Ca(2+), Fe(2+) and Al(3+). CC {ECO:0000269|Ref.1}. CC -!- BIOPHYSICOCHEMICAL PROPERTIES: CC pH dependence: CC Optimum pH is 2.4. Stable at pH 7.2 and 5.2. {ECO:0000269|Ref.1}; CC Temperature dependence: CC Optimum temperature is 20 degrees Celsius. Thermostable, maintains CC activity from 50 to 60 degrees Celsius. {ECO:0000269|Ref.1}; CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.1}. CC -!- MISCELLANEOUS: Inhibits cell proliferation of HepG2 hepatoma cell line CC with an IC(50) of 2.6 uM and L1210 mouse leukemia cell line with an CC IC(50)=3.2 uM respectively. {ECO:0000269|Ref.1}. CC -!- SIMILARITY: Belongs to the multicopper oxidase family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR GO; GO:0005615; C:extracellular space; IDA:UniProtKB. DR GO; GO:0052716; F:hydroquinone:oxygen oxidoreductase activity; IDA:UniProtKB. DR GO; GO:0046274; P:lignin catabolic process; IDA:UniProtKB. PE 1: Evidence at protein level; KW Direct protein sequencing; Lignin degradation; Oxidoreductase; Secreted. FT CHAIN 1..>8 FT /note="Laccase" FT /id="PRO_0000422126" FT NON_TER 8 FT /evidence="ECO:0000303|Ref.1" SQ SEQUENCE 8 AA; 799 MW; 87CB1AB2D768705D CRC64; AIGPVDEV //