ID NCBP2_SALSA Reviewed; 155 AA. AC C0H859; B5DGH3; DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot. DT 05-MAY-2009, sequence version 1. DT 25-MAY-2022, entry version 40. DE RecName: Full=Nuclear cap-binding protein subunit 2; DE AltName: Full=20 kDa nuclear cap-binding protein; DE AltName: Full=NCBP 20 kDa subunit; DE Short=CBP20; GN Name=ncbp2; Synonyms=cbp20; OS Salmo salar (Atlantic salmon). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Actinopterygii; Neopterygii; Teleostei; Protacanthopterygii; Salmoniformes; OC Salmonidae; Salmoninae; Salmo. OX NCBI_TaxID=8030; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RC TISSUE=White muscle; RA Andreassen R., Hoyheim B.; RT "Characterization of full-length sequenced inserts (FLIcs) from a salmo RT salar white muscle specific cDNA library."; RL Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases. RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Brain; RX PubMed=20433749; DOI=10.1186/1471-2164-11-279; RA Leong J.S., Jantzen S.G., von Schalburg K.R., Cooper G.A., Messmer A.M., RA Liao N.Y., Munro S., Moore R., Holt R.A., Jones S.J., Davidson W.S., RA Koop B.F.; RT "Salmo salar and Esox lucius full-length cDNA sequences reveal changes in RT evolutionary pressures on a post-tetraploidization genome."; RL BMC Genomics 11:279-279(2010). CC -!- FUNCTION: Component of the cap-binding complex (CBC), which binds co- CC transcriptionally to the 5' cap of pre-mRNAs and is involved in various CC processes such as pre-mRNA splicing, translation regulation, nonsense- CC mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs CC (miRNAs) and mRNA export. The CBC complex is involved in mRNA export CC from the nucleus, leading to the recruitment of the mRNA export CC machinery to the 5' end of mRNA and to mRNA export in a 5' to 3' CC direction through the nuclear pore. The CBC complex is also involved in CC mediating U snRNA and intronless mRNAs export from the nucleus. The CBC CC complex is essential for a pioneer round of mRNA translation, before CC steady state translation when the CBC complex is replaced by CC cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA CC translation mediated by the CBC complex plays a central role in CC nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs CC bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex CC enhances NMD in mRNAs containing at least one exon-junction complex CC (EJC), promoting the interaction between upf1 and upf2. The CBC complex CC is also involved in 'failsafe' NMD, which is independent of the EJC CC complex, while it does not participate in Staufen-mediated mRNA decay CC (SMD). During cell proliferation, the CBC complex is also involved in CC microRNAs (miRNAs) biogenesis via its interaction with srrt/ars2, CC thereby being required for miRNA-mediated RNA interference. The CBC CC complex also acts as a negative regulator of parn, thereby acting as an CC inhibitor of mRNA deadenylation. In the CBC complex, ncbp2/cbp20 CC recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires CC ncbp1/cbp80 to stabilize the movement of its N-terminal loop and lock CC the CBC into a high affinity cap-binding state with the cap structure. CC The conventional cap-binding complex with NCBP2 binds both small CC nuclear RNA (snRNA) and messenger (mRNA) and is involved in their CC export from the nucleus (By similarity). CC {ECO:0000250|UniProtKB:P52298}. CC -!- SUBUNIT: Component of the nuclear cap-binding complex (CBC), a CC heterodimer composed of ncbp1/cbp80 and ncbp2/cbp20 that interacts with CC m7GpppG-capped RNA. {ECO:0000250|UniProtKB:P52298}. CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P52298}. Cytoplasm CC {ECO:0000250|UniProtKB:P52298}. CC -!- SIMILARITY: Belongs to the RRM NCBP2 family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; BT043732; ACH70847.1; -; mRNA. DR EMBL; BT058515; ACN10228.1; -; mRNA. DR EMBL; BT060223; ACN12583.1; -; mRNA. DR RefSeq; XP_013979037.1; XM_014123562.1. DR AlphaFoldDB; C0H859; -. DR SMR; C0H859; -. DR STRING; 8030.ENSSSAP00000035933; -. DR GeneID; 100196301; -. DR CTD; 22916; -. DR Proteomes; UP000087266; Chromosome ssa10. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0005846; C:nuclear cap binding complex; IEA:InterPro. DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0003729; F:mRNA binding; ISS:UniProtKB. DR GO; GO:0000340; F:RNA 7-methylguanosine cap binding; ISS:UniProtKB. DR GO; GO:0017069; F:snRNA binding; ISS:UniProtKB. DR GO; GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW. DR GO; GO:0045292; P:mRNA cis splicing, via spliceosome; ISS:UniProtKB. DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW. DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IEA:UniProtKB-KW. DR GO; GO:0046833; P:positive regulation of RNA export from nucleus; ISS:UniProtKB. DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW. DR GO; GO:0008380; P:RNA splicing; ISS:UniProtKB. DR GO; GO:0006408; P:snRNA export from nucleus; ISS:UniProtKB. DR CDD; cd12240; RRM_NCBP2; 1. DR Gene3D; 3.30.70.330; -; 1. DR InterPro; IPR027157; NCBP2. DR InterPro; IPR034148; NCBP2_RRM. DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf. DR InterPro; IPR035979; RBD_domain_sf. DR InterPro; IPR000504; RRM_dom. DR PANTHER; PTHR18847; PTHR18847; 1. DR Pfam; PF00076; RRM_1; 1. DR SMART; SM00360; RRM; 1. DR SUPFAM; SSF54928; SSF54928; 1. DR PROSITE; PS50102; RRM; 1. PE 2: Evidence at transcript level; KW Cytoplasm; mRNA processing; mRNA splicing; mRNA transport; KW Nonsense-mediated mRNA decay; Nucleus; Reference proteome; RNA-binding; KW RNA-mediated gene silencing; Translation regulation; Transport. FT CHAIN 1..155 FT /note="Nuclear cap-binding protein subunit 2" FT /id="PRO_0000385253" FT DOMAIN 39..117 FT /note="RRM" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176" FT REGION 111..115 FT /note="mRNA cap binding" FT /evidence="ECO:0000250" FT REGION 122..155 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 122..126 FT /note="mRNA cap binding" FT /evidence="ECO:0000250" FT REGION 132..133 FT /note="mRNA cap binding" FT /evidence="ECO:0000250" FT COMPBIAS 129..143 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT BINDING 19 FT /note="mRNA cap" FT /evidence="ECO:0000250" FT BINDING 42 FT /note="mRNA cap" FT /evidence="ECO:0000250" FT CONFLICT 3 FT /note="S -> I (in Ref. 1; ACH70847)" FT /evidence="ECO:0000305" SQ SEQUENCE 155 AA; 17940 MW; 37E447A35C516D7B CRC64; MSSKLNALFS DSYVDVSQYR DQHFKGNRYE QEKLLKQANT LYVGNLSFYT TEEQVYELFS KSGDVKRIII GLDKVKKTAC GFCFVEYYTR TDAENAMRFV NGTRLDDRII RTDWDAGFKE GRQYGRGKSG GQVRDEYRQD YDPARGGYGK VVSRP //