ID RHPA_HELP8 Reviewed; 492 AA. AC B9XXL6; DT 03-SEP-2014, integrated into UniProtKB/Swiss-Prot. DT 14-APR-2009, sequence version 1. DT 29-OCT-2014, entry version 25. DE RecName: Full=DEAD-box ATP-dependent RNA helicase RhpA; DE EC=3.6.4.13; GN Name=rhpA; ORFNames=HPB128_21g22; OS Helicobacter pylori (strain B128). OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales; OC Helicobacteraceae; Helicobacter. OX NCBI_TaxID=544406; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=B128; RX PubMed=19123947; DOI=10.1186/1471-2164-10-3; RA McClain M.S., Shaffer C.L., Israel D.A., Peek R.M. Jr., Cover T.L.; RT "Genome sequence analysis of Helicobacter pylori strains associated RT with gastric ulceration and gastric cancer."; RL BMC Genomics 10:3-3(2009). RN [2] RP FUNCTION AS AN ATPASE, INTERACTION WITH RNJ, SUBUNIT, SUBCELLULAR RP LOCATION, AND DISRUPTION PHENOTYPE. RC STRAIN=B128; RX PubMed=23093592; DOI=10.1093/nar/gks945; RA Redko Y., Aubert S., Stachowicz A., Lenormand P., Namane A., RA Darfeuille F., Thibonnier M., De Reuse H.; RT "A minimal bacterial RNase J-based degradosome is associated with RT translating ribosomes."; RL Nucleic Acids Res. 41:288-301(2013). CC -!- FUNCTION: DEAD-box RNA helicase probably involved in RNA CC degradation. Unwinds dsRNA in both 5'- and 3'-directions (By CC similarity). Background RNA-dependent ATPase activity is CC stimulated about 5-fold by RNaseJ (rnj). Stimulates the dsRNase CC activity of RNase J. {ECO:0000250, ECO:0000269|PubMed:23093592}. CC -!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate. CC -!- SUBUNIT: Homodimer (By similarity). Interacts with RNase J (rnj), CC might be a member of a minimal RNA degradosome complex. CC {ECO:0000250, ECO:0000269|PubMed:23093592}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:23093592}. CC Note=The RNaseJ-RhpA complex co-localizes with 70S ribosomes and CC polysomes; remains associated with ribosomes in the absence of CC RNase J. CC -!- DISRUPTION PHENOTYPE: Not essential, it can be deleted. RNase J CC remains associated with the ribosomes and polysomes. CC {ECO:0000269|PubMed:23093592}. CC -!- SIMILARITY: Belongs to the DEAD box helicase family. CC {ECO:0000305}. CC -!- SIMILARITY: Contains 1 helicase ATP-binding domain. CC {ECO:0000255|PROSITE-ProRule:PRU00541}. CC -!- SIMILARITY: Contains 1 helicase C-terminal domain. CC {ECO:0000255|PROSITE-ProRule:PRU00542}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; ABSY01000001; EEC25260.1; -; Genomic_DNA. DR ProteinModelPortal; B9XXL6; -. DR EnsemblBacteria; EEC25260; EEC25260; HPB128_21g22. DR PATRIC; 27522829; VBIHelPyl107473_0023. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW. DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW. DR Gene3D; 3.40.50.300; -; 2. DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom. DR InterPro; IPR014001; Helicase_ATP-bd. DR InterPro; IPR001650; Helicase_C. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif. DR Pfam; PF00270; DEAD; 1. DR Pfam; PF00271; Helicase_C; 1. DR SMART; SM00487; DEXDc; 1. DR SMART; SM00490; HELICc; 1. DR SUPFAM; SSF52540; SSF52540; 1. DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1. DR PROSITE; PS51194; HELICASE_CTER; 1. DR PROSITE; PS51195; Q_MOTIF; 1. PE 1: Evidence at protein level; KW ATP-binding; Cytoplasm; Helicase; Hydrolase; Nucleotide-binding; KW RNA-binding. FT CHAIN 1 492 DEAD-box ATP-dependent RNA helicase RhpA. FT /FTId=PRO_0000430106. FT DOMAIN 51 220 Helicase ATP-binding. FT {ECO:0000255|PROSITE-ProRule:PRU00541}. FT DOMAIN 231 393 Helicase C-terminal. FT {ECO:0000255|PROSITE-ProRule:PRU00542}. FT NP_BIND 64 71 ATP. {ECO:0000255|PROSITE- FT ProRule:PRU00541}. FT MOTIF 20 48 Q motif. FT MOTIF 168 171 DEAD box. SQ SEQUENCE 492 AA; 55845 MW; 0EA636F0110D3183 CRC64; MELNQPPLPT EIDDDAYHKP SFNDLGLKES VLKSVYEAGF TSPSPIQEKA IPAVLQGRDV IAQAQTGTGK TAAFALPIIN NLKNNHTIEA LVITPTRELA MQISDEIFKL GKHTRTKTVC VYGGQSVKKQ CEFIKKNPQV MIATPGRLLD HLKNERIHKF VPKVVVLDES DEMLDMGFLD DIEEIFDYLP SEAQILLFSA TMPEPIKRLA DKILENPIKI HIAPSNITNT DITQRFYVIN EHERAEAIMR LLDTQAPKKS IVFTRTKKEA DELHQFLASK NYKSTALHGD MDQRDRRASI MAFKKNDADV LVATDVASRG LDISGVSHVF NYHLPLNTES YIHRIGRTGR AGKKGMAITL VTPLEYKELL RMQKEIDSEI ELFEIPTINE NQIIKTLHDA KVSEGIISLY EQLTEIFEPS QLVLKLLSLQ FETSKIGLNQ QEIDAIQNPK EKTPKPSHKK TPQHERARSF KKGQHRDRHP KTNHHSKKPK RR //