ID IF2_BACCQ Reviewed; 686 AA. AC B9IVA1; DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot. DT 24-MAR-2009, sequence version 1. DT 03-SEP-2014, entry version 42. DE RecName: Full=Translation initiation factor IF-2; GN Name=infB; OrderedLocusNames=BCQ_3597; OS Bacillus cereus (strain Q1). OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; OC Bacillus cereus group. OX NCBI_TaxID=361100; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Q1; RX PubMed=19060151; DOI=10.1128/JB.01629-08; RA Xiong Z., Jiang Y., Qi D., Lu H., Yang F., Yang J., Chen L., Sun L., RA Xu X., Xue Y., Zhu Y., Jin Q.; RT "Complete genome sequence of the extremophilic Bacillus cereus strain RT Q1 with industrial applications."; RL J. Bacteriol. 191:1120-1121(2009). CC -!- FUNCTION: One of the essential components for the initiation of CC protein synthesis. Protects formylmethionyl-tRNA from spontaneous CC hydrolysis and promotes its binding to the 30S ribosomal subunits. CC Also involved in the hydrolysis of GTP during the formation of the CC 70S ribosomal complex (By similarity). CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity). CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase CC superfamily. Classic translation factor GTPase family. IF-2 CC subfamily. CC -!- SIMILARITY: Contains 1 tr-type G (guanine nucleotide-binding) CC domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP000227; ACM14025.1; -; Genomic_DNA. DR RefSeq; YP_002531314.1; NC_011969.1. DR STRING; 361100.BCQ_3597; -. DR EnsemblBacteria; ACM14025; ACM14025; BCQ_3597. DR GeneID; 7372445; -. DR KEGG; bcq:BCQ_3597; -. DR PATRIC; 18914916; VBIBacCer120424_3626. DR eggNOG; COG0532; -. DR HOGENOM; HOG000076906; -. DR KO; K02519; -. DR OMA; IYKAIDD; -. DR OrthoDB; EOG67HJSV; -. DR BioCyc; BCER361100:GJ7M-3585-MONOMER; -. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW. DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-HAMAP. DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-HAMAP. DR Gene3D; 3.40.50.10050; -; 1. DR Gene3D; 3.40.50.300; -; 1. DR HAMAP; MF_00100_B; IF_2_B; 1. DR InterPro; IPR000795; EF_GTP-bd_dom. DR InterPro; IPR006847; IF2_N. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR005225; Small_GTP-bd_dom. DR InterPro; IPR000178; TF_IF2_bacterial-like. DR InterPro; IPR015760; TIF_IF2. DR InterPro; IPR023115; TIF_IF2_dom3. DR InterPro; IPR009000; Transl_B-barrel. DR InterPro; IPR004161; Transl_elong_EFTu/EF1A_2. DR PANTHER; PTHR23115:SF41; PTHR23115:SF41; 1. DR Pfam; PF00009; GTP_EFTU; 1. DR Pfam; PF03144; GTP_EFTU_D2; 1. DR Pfam; PF11987; IF-2; 1. DR Pfam; PF04760; IF2_N; 2. DR SUPFAM; SSF50447; SSF50447; 2. DR SUPFAM; SSF52156; SSF52156; 1. DR SUPFAM; SSF52540; SSF52540; 1. DR TIGRFAMs; TIGR00487; IF-2; 1. DR TIGRFAMs; TIGR00231; small_GTP; 1. DR PROSITE; PS51722; G_TR_2; 1. DR PROSITE; PS01176; IF2; 1. PE 3: Inferred from homology; KW Complete proteome; Cytoplasm; GTP-binding; Initiation factor; KW Nucleotide-binding; Protein biosynthesis. FT CHAIN 1 686 Translation initiation factor IF-2. FT /FTId=PRO_1000118749. FT DOMAIN 188 357 tr-type G. FT NP_BIND 197 204 GTP (By similarity). FT NP_BIND 243 247 GTP (By similarity). FT NP_BIND 297 300 GTP (By similarity). FT REGION 197 204 G1 (By similarity). FT REGION 222 226 G2 (By similarity). FT REGION 243 246 G3 (By similarity). FT REGION 297 300 G4 (By similarity). FT REGION 333 335 G5 (By similarity). SQ SEQUENCE 686 AA; 75726 MW; E981BBB3FA1A9A00 CRC64; MSKIRVHEYA KKHNISSKDL MTKLKEMNIE VSNHMTMLDD EVVNKLDNEY QAEKPSVADE FEVEEKVVRS KKNSNKKKKK GKGNEDKRQE NFAGRQQTQT VETPDKITFS GSLTVGDLAK KLSKEPSEII KKLFMLGIMA TINQDLDKDT IELIANDYGI EVEEEVIVSE TEFETFIDEQ DDEENLKERP AVVTIMGHVD HGKTTLLDSI RNSKVTAGEA GGITQHIGAY QVEVNDKKIT FLDTPGHAAF TTMRARGAQV TDITILVVAA DDGVMPQTVE AINHAKAAGV PIIVAVNKMD KPAANPDRVM QELTEYELVP EAWGGDTIFV PISAIQGEGI DNLLEMILLV SEVEEYKANP NRYATGTVIE AQLDKGKGTI ATLLVQNGTL RVGDPIVVGT TFGRVRAMVS DIGRRVKVAG PSTPVEITGL NEVPQAGDRF MAFADEKKAR QIGESRAQEA LLAQRGEKSK LSLEDLFQQI QEGDVKEINL IVKADVQGSV EAMAASLRKI DVEGVKVKII HTGVGAITES DIILASASNA IVIGFNVRPD VNAKRTAELE NVDIRLHRII YKVIEEIEAA MQGMLDPEFE EKVIGQAEVR QTFKVTKVGT IAGCYVTDGK ITRDSGVRII RDGVVIYEGQ LDTLKRFKDD VKEVAQNYEC GITIEKYNDL KEGDIIEAYI MEEVKR //