ID B9HM36_POPTR Unreviewed; 212 AA. AC B9HM36; DT 24-MAR-2009, integrated into UniProtKB/TrEMBL. DT 24-MAR-2009, sequence version 1. DT 12-OCT-2022, entry version 82. DE RecName: Full=Glutathione S-transferase DHAR2 {ECO:0000256|Google:UnProtein}; GN ORFNames=POPTR_008G049300 {ECO:0000313|EMBL:PNT22804.1}; OS Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. OS trichocarpa). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Malpighiales; Salicaceae; Saliceae; Populus. OX NCBI_TaxID=3694 {ECO:0000313|EMBL:PNT22804.1, ECO:0000313|Proteomes:UP000006729}; RN [1] {ECO:0000313|EMBL:PNT22804.1, ECO:0000313|Proteomes:UP000006729} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Nisqually {ECO:0000313|Proteomes:UP000006729}; RX PubMed=16973872; DOI=10.1126/science.1128691; RA Tuskan G.A., Difazio S., Jansson S., Bohlmann J., Grigoriev I., RA Hellsten U., Putnam N., Ralph S., Rombauts S., Salamov A., Schein J., RA Sterck L., Aerts A., Bhalerao R.R., Bhalerao R.P., Blaudez D., Boerjan W., RA Brun A., Brunner A., Busov V., Campbell M., Carlson J., Chalot M., RA Chapman J., Chen G.L., Cooper D., Coutinho P.M., Couturier J., Covert S., RA Cronk Q., Cunningham R., Davis J., Degroeve S., Dejardin A., RA Depamphilis C., Detter J., Dirks B., Dubchak I., Duplessis S., Ehlting J., RA Ellis B., Gendler K., Goodstein D., Gribskov M., Grimwood J., Groover A., RA Gunter L., Hamberger B., Heinze B., Helariutta Y., Henrissat B., RA Holligan D., Holt R., Huang W., Islam-Faridi N., Jones S., RA Jones-Rhoades M., Jorgensen R., Joshi C., Kangasjarvi J., Karlsson J., RA Kelleher C., Kirkpatrick R., Kirst M., Kohler A., Kalluri U., Larimer F., RA Leebens-Mack J., Leple J.C., Locascio P., Lou Y., Lucas S., Martin F., RA Montanini B., Napoli C., Nelson D.R., Nelson C., Nieminen K., Nilsson O., RA Pereda V., Peter G., Philippe R., Pilate G., Poliakov A., Razumovskaya J., RA Richardson P., Rinaldi C., Ritland K., Rouze P., Ryaboy D., Schmutz J., RA Schrader J., Segerman B., Shin H., Siddiqui A., Sterky F., Terry A., RA Tsai C.J., Uberbacher E., Unneberg P., Vahala J., Wall K., Wessler S., RA Yang G., Yin T., Douglas C., Marra M., Sandberg G., Van de Peer Y., RA Rokhsar D.; RT "The genome of black cottonwood, Populus trichocarpa (Torr. & Gray)."; RL Science 313:1596-1604(2006). RN [2] {ECO:0007829|PDB:2N5F, ECO:0007829|PDB:5MYE} RP STRUCTURE BY NMR IN COMPLEX WITH GLUTATHIONE. RX PubMed=26699905; DOI=10.1042/BJ20151147; RA Lallement P.A., Roret T., Tsan P., Gualberto J.M., Girardet J.M., RA Didierjean C., Rouhier N., Hecker A.; RT "Insights into ascorbate regeneration in plants: investigating the redox RT and structural properties of dehydroascorbate reductases from Populus RT trichocarpa."; RL Biochem. J. 473:717-731(2016). CC -!- CATALYTIC ACTIVITY: CC Reaction=2 glutathione + L-dehydroascorbate = glutathione disulfide + CC L-ascorbate; Xref=Rhea:RHEA:24424, ChEBI:CHEBI:38290, CC ChEBI:CHEBI:57925, ChEBI:CHEBI:58297, ChEBI:CHEBI:58539; EC=1.8.5.1; CC Evidence={ECO:0000256|ARBA:ARBA00000509}; CC -!- SIMILARITY: Belongs to the GST superfamily. DHAR family. CC {ECO:0000256|ARBA:ARBA00024194}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CM009297; PNT22804.1; -; Genomic_DNA. DR RefSeq; XP_002311137.1; XM_002311101.2. DR PDB; 2N5F; NMR; -; A=1-212. DR PDB; 5MYE; NMR; -; A=1-212. DR PDB; 5N9U; NMR; -; A=1-212. DR PDBsum; 2N5F; -. DR PDBsum; 5MYE; -. DR PDBsum; 5N9U; -. DR BMRB; B9HM36; -. DR SMR; B9HM36; -. DR STRING; 3694.POPTR_0008s04920.1; -. DR EnsemblPlants; PNT22804; PNT22804; POPTR_008G049300v3. DR GeneID; 7469797; -. DR Gramene; PNT22804; PNT22804; POPTR_008G049300v3. DR KEGG; pop:7469797; -. DR eggNOG; KOG1422; Eukaryota. DR InParanoid; B9HM36; -. DR OMA; EEIHIAY; -. DR Proteomes; UP000006729; Chromosome 8. DR ExpressionAtlas; B9HM36; baseline. DR GO; GO:0045174; F:glutathione dehydrogenase (ascorbate) activity; IBA:GO_Central. DR GO; GO:0004364; F:glutathione transferase activity; IEA:UniProtKB-EC. DR GO; GO:0033355; P:ascorbate glutathione cycle; IEA:InterPro. DR GO; GO:0010731; P:protein glutathionylation; IBA:GO_Central. DR InterPro; IPR044627; DHAR1/2/3/4. DR InterPro; IPR010987; Glutathione-S-Trfase_C-like. DR InterPro; IPR036282; Glutathione-S-Trfase_C_sf. DR InterPro; IPR040079; Glutathione_S-Trfase. DR InterPro; IPR004045; Glutathione_S-Trfase_N. DR InterPro; IPR036249; Thioredoxin-like_sf. DR PANTHER; PTHR44420; PTHR44420; 1. DR Pfam; PF13417; GST_N_3; 1. DR SFLD; SFLDS00019; Glutathione_Transferase_(cytos; 1. DR SUPFAM; SSF47616; SSF47616; 1. DR SUPFAM; SSF52833; SSF52833; 1. DR PROSITE; PS50405; GST_CTER; 1. DR PROSITE; PS50404; GST_NTER; 1. PE 1: Evidence at protein level; KW 3D-structure {ECO:0007829|PDB:2N5F, ECO:0007829|PDB:5MYE}; KW Reference proteome {ECO:0000313|Proteomes:UP000006729}. FT DOMAIN 10..88 FT /note="GST N-terminal" FT /evidence="ECO:0000259|PROSITE:PS50404" FT DOMAIN 66..212 FT /note="GST C-terminal" FT /evidence="ECO:0000259|PROSITE:PS50405" SQ SEQUENCE 212 AA; 23533 MW; C8DF44B22C94D1AF CRC64; MALEICVKAA VGAPNILGDC PFCQRVLLSL EEKKIPYKSY LINLGDKPQW FLEISPEGKV PVVKIDDKWV ADSDVIVGIL EEKNPEPPLA TPPEFASVGS KIFPSFVKFL KSKDPNDGTE QALLEELKAL DGHLKVHGPF IAGEKITAVD LSLAPKLYHL EVALGHFKNW TIPDNLTHVL NYIKLLFSRE SFKKTRAAEE HVIAGWEPKV NA //