ID   B9EI38_MOUSE            Unreviewed;       648 AA.
AC   B9EI38;
DT   24-MAR-2009, integrated into UniProtKB/TrEMBL.
DT   24-MAR-2009, sequence version 1.
DT   02-APR-2025, entry version 106.
DE   SubName: Full=RALBP1 associated Eps domain containing protein 2 {ECO:0000313|EMBL:AAI39013.1, ECO:0000313|Ensembl:ENSMUSP00000098661.2};
GN   Name=Reps2 {ECO:0000313|EMBL:AAI39013.1,
GN   ECO:0000313|Ensembl:ENSMUSP00000098661.2,
GN   ECO:0000313|MGI:MGI:2663511};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090 {ECO:0000313|EMBL:AAI39013.1};
RN   [1] {ECO:0000313|EMBL:AAI39013.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testicle {ECO:0000313|EMBL:AAI39013.1};
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RA   Gerhard D.S., Wagner L., Feingold E.A., Shenmen C.M., Grouse L.H.,
RA   Schuler G., Klein S.L., Old S., Rasooly R., Good P., Guyer M., Peck A.M.,
RA   Derge J.G., Lipman D., Collins F.S., Jang W., Sherry S., Feolo M.,
RA   Misquitta L., Lee E., Rotmistrovsky K., Greenhut S.F., Schaefer C.F.,
RA   Buetow K., Bonner T.I., Haussler D., Kent J., Kiekhaus M., Furey T.,
RA   Brent M., Prange C., Schreiber K., Shapiro N., Bhat N.K., Hopkins R.F.,
RA   Hsie F., Driscoll T., Soares M.B., Casavant T.L., Scheetz T.E.,
RA   Brown-stein M.J., Usdin T.B., Toshiyuki S., Carninci P., Piao Y.,
RA   Dudekula D.B., Ko M.S., Kawakami K., Suzuki Y., Sugano S., Gruber C.E.,
RA   Smith M.R., Simmons B., Moore T., Waterman R., Johnson S.L., Ruan Y.,
RA   Wei C.L., Mathavan S., Gunaratne P.H., Wu J., Garcia A.M., Hulyk S.W.,
RA   Fuh E., Yuan Y., Sneed A., Kowis C., Hodgson A., Muzny D.M., McPherson J.,
RA   Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S.,
RA   Sanchez A., Whiting M., Madari A., Young A.C., Wetherby K.D., Granite S.J.,
RA   Kwong P.N., Brinkley C.P., Pearson R.L., Bouffard G.G., Blakesly R.W.,
RA   Green E.D., Dickson M.C., Rodriguez A.C., Grimwood J., Schmutz J.,
RA   Myers R.M., Butterfield Y.S., Griffith M., Griffith O.L., Krzywinski M.I.,
RA   Liao N., Morin R., Morrin R., Palmquist D., Petrescu A.S., Skalska U.,
RA   Smailus D.E., Stott J.M., Schnerch A., Schein J.E., Jones S.J., Holt R.A.,
RA   Baross A., Marra M.A., Clifton S., Makowski K.A., Bosak S., Malek J.;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [2] {ECO:0007829|PubMed:17242355}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA   Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT   "Large-scale phosphorylation analysis of mouse liver.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN   [3] {ECO:0000313|Ensembl:ENSMUSP00000098661.2, ECO:0000313|Proteomes:UP000000589}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000098661.2,
RC   ECO:0000313|Proteomes:UP000000589};
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RG   Mouse Genome Sequencing Consortium;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [4] {ECO:0007829|PubMed:21183079}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
RN   [5] {ECO:0000313|Ensembl:ENSMUSP00000098661.2}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000098661.2};
RX   PubMed=21750661;
RA   Church D.M., Schneider V.A., Graves T., Auger K., Cunningham F., Bouk N.,
RA   Chen H.C., Agarwala R., McLaren W.M., Ritchie G.R., Albracht D.,
RA   Kremitzki M., Rock S., Kotkiewicz H., Kremitzki C., Wollam A., Trani L.,
RA   Fulton L., Fulton R., Matthews L., Whitehead S., Chow W., Torrance J.,
RA   Dunn M., Harden G., Threadgold G., Wood J., Collins J., Heath P.,
RA   Griffiths G., Pelan S., Grafham D., Eichler E.E., Weinstock G.,
RA   Mardis E.R., Wilson R.K., Howe K., Flicek P., Hubbard T.;
RT   "Modernizing reference genome assemblies.";
RL   PLoS Biol. 9:e1001091-e1001091(2011).
RN   [6] {ECO:0000313|Ensembl:ENSMUSP00000098661.2}
RP   IDENTIFICATION.
RC   STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000098661.2};
RG   Ensembl;
RL   Submitted (DEC-2024) to UniProtKB.
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DR   EMBL; BC139012; AAI39013.1; -; mRNA.
DR   RefSeq; NP_839987.2; NM_178256.5.
DR   SMR; B9EI38; -.
DR   ProteomicsDB; 332961; -.
DR   Antibodypedia; 434; 143 antibodies from 27 providers.
DR   Ensembl; ENSMUST00000101102.2; ENSMUSP00000098661.2; ENSMUSG00000040855.16.
DR   GeneID; 194590; -.
DR   UCSC; uc009uue.2; mouse.
DR   AGR; MGI:2663511; -.
DR   CTD; 9185; -.
DR   MGI; MGI:2663511; Reps2.
DR   VEuPathDB; HostDB:ENSMUSG00000040855; -.
DR   GeneTree; ENSGT00940000158080; -.
DR   HOGENOM; CLU_014864_1_0_1; -.
DR   OMA; EEVWIIS; -.
DR   OrthoDB; 10045710at2759; -.
DR   TreeFam; TF316546; -.
DR   BioGRID-ORCS; 194590; 3 hits in 75 CRISPR screens.
DR   ChiTaRS; Reps2; mouse.
DR   Proteomes; UP000000589; Chromosome X.
DR   Bgee; ENSMUSG00000040855; Expressed in medial dorsal nucleus of thalamus and 180 other cell types or tissues.
DR   ExpressionAtlas; B9EI38; baseline and differential.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   CDD; cd00052; EH; 1.
DR   FunFam; 1.10.238.10:FF:000039; RalBP1-associated Eps domain-containing protein 2 isoform 1; 1.
DR   Gene3D; 1.10.238.10; EF-hand; 2.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   InterPro; IPR002048; EF_hand_dom.
DR   InterPro; IPR000261; EH_dom.
DR   PANTHER; PTHR11216; EH DOMAIN; 1.
DR   PANTHER; PTHR11216:SF64; RALBP1-ASSOCIATED EPS DOMAIN-CONTAINING PROTEIN 2; 1.
DR   Pfam; PF12763; EF-hand_4; 1.
DR   SMART; SM00027; EH; 1.
DR   SUPFAM; SSF47473; EF-hand; 2.
DR   PROSITE; PS00018; EF_HAND_1; 1.
DR   PROSITE; PS50222; EF_HAND_2; 1.
DR   PROSITE; PS50031; EH; 2.
PE   1: Evidence at protein level;
KW   Calcium {ECO:0000256|ARBA:ARBA00022837};
KW   Coiled coil {ECO:0000256|SAM:Coils};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Proteomics identification {ECO:0007829|PeptideAtlas:B9EI38,
KW   ECO:0007829|ProteomicsDB:B9EI38};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000589}.
FT   DOMAIN          21..122
FT                   /note="EH"
FT                   /evidence="ECO:0000259|PROSITE:PS50031"
FT   DOMAIN          269..354
FT                   /note="EH"
FT                   /evidence="ECO:0000259|PROSITE:PS50031"
FT   DOMAIN          302..337
FT                   /note="EF-hand"
FT                   /evidence="ECO:0000259|PROSITE:PS50222"
FT   REGION          161..234
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          403..479
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          493..569
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          600..641
FT                   /evidence="ECO:0000256|SAM:Coils"
FT   COMPBIAS        161..170
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        222..231
FT                   /note="Low complexity"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        500..511
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        525..540
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   648 AA;  70612 MW;  D2232FFA8FAB3817 CRC64;
     MEVAAGGGCG AGPPLLLSDS EQQCYSELFA RCAGAASGGP GPGPPEATRV APGTATAAAG
     PVADLFRASQ LPPETLHQIT ELCGAKRVGY FGPTQFYVAL KLIAAAQAGL PVRTESIKCE
     LPLPRFVMSK NDGEIRFGNP AELHGPKVQI PYLTTEKNSF KRMDNEDKQQ ETQSPTMSPL
     ASPPSSPPHY QRVSLSHGYS KLRSGTEQMH PAPYERQPIG QPEGPSSEGP GAKPFRRQAS
     LIRSFSVERE PQENNSNYPD EPWRITEEQR EYYVNQFRSL QPDPSSFISG SVAKNFFTKS
     KLSIPELSYI WELSDADCDG ALTLSEFCAA FHLIVARKNG YPLPEGLPPT LQPEYLQAAF
     PKSKWECAIF DSYSESMPAN QQSCDLNRME KTSVKDVADF PVPTQDVTTA DDKQALKSTV
     NESLPKDVSE DTATSKDYNS LKARPRSRSY SSTSIEEAMK RGEDPPTPPP RPQKTHSRAS
     SLDLNKVFLP SAPAANSGLL PPPPALPPRP CPTQSEPVSE ADLHSQLNRA PSQAAESSPT
     KMDAPHAQPP SKPIRRKFRP ENQTTESQEP AAAVGGAVSA AMVKPHPTVQ KQSSKQKKAI
     QTAIRKNKEA NAVLARLNSE LQQQLKEVHQ ERIALENQLE QLRPVTVL
//