ID B7NWJ5_9INFA Unreviewed; 705 AA. AC B7NWJ5; DT 10-FEB-2009, integrated into UniProtKB/TrEMBL. DT 10-FEB-2009, sequence version 1. DT 24-JUL-2024, entry version 55. DE RecName: Full=RNA-directed RNA polymerase catalytic subunit {ECO:0000256|ARBA:ARBA00020035, ECO:0000256|RuleBase:RU004330}; DE EC=2.7.7.48 {ECO:0000256|ARBA:ARBA00012494, ECO:0000256|RuleBase:RU004330}; DE Flags: Fragment; GN Name=PB1 {ECO:0000313|EMBL:ACJ26173.1}; OS Influenza A virus (A/common kestrel/Hong Kong/2372/2007(H5N1)). OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; OC Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus; OC Alphainfluenzavirus influenzae; Influenza A virus. OX NCBI_TaxID=573957 {ECO:0000313|EMBL:ACJ26173.1}; RN [1] {ECO:0000313|EMBL:ACJ26173.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=A/common kestrel/Hong Kong/2372/2007 RC {ECO:0000313|EMBL:ACJ26173.1}; RX PubMed=19239752; DOI=10.3201/eid1503.081190; RA Smith G.J., Vijaykrishna D., Ellis T.M., Dyrting K.C., Leung Y.H., Bahl J., RA Wong C.W., Kai H., Chow M.K., Duan L., Chan A.S., Zhang L.J., Chen H., RA Luk G.S., Peiris J.S., Guan Y.; RT "Characterization of avian influenza viruses A (H5N1) from wild birds, Hong RT Kong, 2004-2008."; RL Emerg. Infect. Dis. 15:402-407(2009). CC -!- FUNCTION: RNA-dependent RNA polymerase which is responsible for CC replication and transcription of virus RNA segments. The transcription CC of viral mRNAs occurs by a unique mechanism called cap-snatching. 5' CC methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides CC by PA. In turn, these short capped RNAs are used as primers by PB1 for CC transcription of viral mRNAs. During virus replication, PB1 initiates CC RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn CC serves as a template for the production of more vRNAs. CC {ECO:0000256|ARBA:ARBA00002148}. CC -!- CATALYTIC ACTIVITY: CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA- CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; CC EC=2.7.7.48; Evidence={ECO:0000256|RuleBase:RU004330}; CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. Host CC cytoplasm {ECO:0000256|ARBA:ARBA00004192}. Host nucleus CC {ECO:0000256|ARBA:ARBA00004147}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CY036123; ACJ26173.1; -; Viral_cRNA. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW. DR GO; GO:0003723; F:RNA binding; IEA:InterPro. DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW. DR GO; GO:0039523; P:symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity; IEA:UniProtKB-KW. DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro. DR GO; GO:0019083; P:viral transcription; IEA:UniProtKB-KW. DR Gene3D; 6.10.140.720; -; 1. DR InterPro; IPR007099; RNA-dir_pol_NSvirus. DR InterPro; IPR001407; RNA_pol_PB1_influenza. DR Pfam; PF00602; Flu_PB1; 1. DR PROSITE; PS50525; RDRP_SSRNA_NEG_SEG; 1. PE 4: Predicted; KW Eukaryotic host gene expression shutoff by virus KW {ECO:0000256|ARBA:ARBA00023247}; KW Eukaryotic host transcription shutoff by virus KW {ECO:0000256|ARBA:ARBA00022731}; KW Host cytoplasm {ECO:0000256|ARBA:ARBA00023200}; KW Host gene expression shutoff by virus {ECO:0000256|ARBA:ARBA00022995}; KW Host nucleus {ECO:0000256|ARBA:ARBA00022562}; KW Host-virus interaction {ECO:0000256|ARBA:ARBA00022581}; KW Inhibition of host RNA polymerase II by virus KW {ECO:0000256|ARBA:ARBA00023103}; KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741}; KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695}; KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553}; KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484, KW ECO:0000256|RuleBase:RU004330}; KW Transferase {ECO:0000256|ARBA:ARBA00022484, ECO:0000256|RuleBase:RU004330}; KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953}; KW Viral transcription {ECO:0000256|ARBA:ARBA00023314}. FT DOMAIN 274..471 FT /note="RdRp catalytic" FT /evidence="ECO:0000259|PROSITE:PS50525" FT REGION 38..70 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 38..53 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT NON_TER 1 FT /evidence="ECO:0000313|EMBL:ACJ26173.1" FT NON_TER 705 FT /evidence="ECO:0000313|EMBL:ACJ26173.1" SQ SEQUENCE 705 AA; 80367 MW; B38E84904A130BB1 CRC64; PVQNAISTTF PYTGDPPYSH GTGTGYTMDT VNRTHQYSEK GKWTTNTETG APQLNPIDGP LPEDNEPSGY AQTDCVLEAM AFLEESHPGI FENSCLETME IVQQTRVDKL TQGRQTYDWT LNRNQPAATA LANTIEVFRS NGLTANESGR LIDFLKDVIE SMDKEEMEIT THFQRKRRVR DNMTKKMVTQ RTIGKKKQRL NKRSYLIRAL TLNTMTKDAE RGKLKRRAIA TPGMQIRGFV YFVETLARSI CEKLEQSGLP VGGNEKKAKL ANVVRKMMTN SQDTELSFTI TGDNTKWNEN QNPRMFLAMI TYITRNQPEW FRNVLSIAPI MFSNKMARLG KGYMFESKSM KLRTQIPAEM LANIDLKYFN ELTKKKIEKI RPLLIDGTAS LSPGMMMGMF NMLSTVLGVS ILNLGQKRYT KTTYWWDGLQ SSDDFALIVN APNHEGIQAG VDRFYRTCKL VGINMSKKKS YINRTGTFEF TSFFYRYGFV ANFSMELPSF GVSGINESAD MSIGVTVIKN NMINNDLGPA TAQMALQLFI KDYRYTYRCH RGDTQIQTRR SFELKKLWEQ TRSKAGLLVS DGGPNLYNIR NLHIPEVCLK WELMDEDYQG RLCNPLNPFV SHKEIESVNN AIVMPAHGPA KSMEYDAVAT THSWIPKRNR SILNTSQRGI LEDEQMYQKC CNLFEKFFPS SSYRRPVGIS SMVES //