ID ERA_HELP2 Reviewed; 301 AA. AC B6JL99; DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot. DT 16-DEC-2008, sequence version 1. DT 29-MAY-2024, entry version 74. DE RecName: Full=GTPase Era {ECO:0000255|HAMAP-Rule:MF_00367}; GN Name=era {ECO:0000255|HAMAP-Rule:MF_00367}; OrderedLocusNames=HPP12_0523; OS Helicobacter pylori (strain P12). OC Bacteria; Campylobacterota; Epsilonproteobacteria; Campylobacterales; OC Helicobacteraceae; Helicobacter. OX NCBI_TaxID=570508; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=P12; RA Fischer W., Windhager L., Karnholz A., Zeiller M., Zimmer R., Haas R.; RT "The complete genome sequence of Helicobacter pylori strain P12."; RL Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: An essential GTPase that binds both GDP and GTP, with rapid CC nucleotide exchange. Plays a role in 16S rRNA processing and 30S CC ribosomal subunit biogenesis and possibly also in cell cycle regulation CC and energy metabolism. {ECO:0000255|HAMAP-Rule:MF_00367}. CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00367}. CC -!- SUBCELLULAR LOCATION: Cytoplasm. Cell inner membrane CC {ECO:0000255|HAMAP-Rule:MF_00367}; Peripheral membrane protein CC {ECO:0000255|HAMAP-Rule:MF_00367}. CC -!- SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like CC GTPase superfamily. Era GTPase family. {ECO:0000255|HAMAP- CC Rule:MF_00367, ECO:0000255|PROSITE-ProRule:PRU01050}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP001217; ACJ07677.1; -; Genomic_DNA. DR AlphaFoldDB; B6JL99; -. DR SMR; B6JL99; -. DR KEGG; hpp:HPP12_0523; -. DR HOGENOM; CLU_038009_1_0_7; -. DR Proteomes; UP000008198; Chromosome. DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter. DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell. DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule. DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule. DR GO; GO:0043024; F:ribosomal small subunit binding; IEA:TreeGrafter. DR GO; GO:0070181; F:small ribosomal subunit rRNA binding; IEA:UniProtKB-UniRule. DR GO; GO:0000028; P:ribosomal small subunit assembly; IEA:TreeGrafter. DR CDD; cd04163; Era; 1. DR CDD; cd22534; KH-II_Era; 1. DR Gene3D; 3.30.300.20; -; 1. DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1. DR HAMAP; MF_00367; GTPase_Era; 1. DR InterPro; IPR030388; G_ERA_dom. DR InterPro; IPR006073; GTP-bd. DR InterPro; IPR005662; GTPase_Era. DR InterPro; IPR015946; KH_dom-like_a/b. DR InterPro; IPR004044; KH_dom_type_2. DR InterPro; IPR009019; KH_sf_prok-type. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR005225; Small_GTP-bd_dom. DR NCBIfam; TIGR00436; era; 1. DR NCBIfam; TIGR00231; small_GTP; 1. DR PANTHER; PTHR42698; GTPASE ERA; 1. DR PANTHER; PTHR42698:SF2; GTPASE ERA-LIKE, CHLOROPLASTIC; 1. DR Pfam; PF07650; KH_2; 1. DR Pfam; PF01926; MMR_HSR1; 1. DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1. DR SUPFAM; SSF54814; Prokaryotic type KH domain (KH-domain type II); 1. DR PROSITE; PS51713; G_ERA; 1. DR PROSITE; PS50823; KH_TYPE_2; 1. PE 3: Inferred from homology; KW Cell inner membrane; Cell membrane; Cytoplasm; GTP-binding; Membrane; KW Nucleotide-binding; Ribosome biogenesis; RNA-binding; rRNA-binding. FT CHAIN 1..301 FT /note="GTPase Era" FT /id="PRO_1000121331" FT DOMAIN 4..173 FT /note="Era-type G" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050" FT DOMAIN 204..280 FT /note="KH type-2" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00367" FT REGION 12..19 FT /note="G1" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050" FT REGION 38..42 FT /note="G2" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050" FT REGION 64..67 FT /note="G3" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050" FT REGION 122..125 FT /note="G4" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050" FT REGION 152..154 FT /note="G5" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050" FT BINDING 12..19 FT /ligand="GTP" FT /ligand_id="ChEBI:CHEBI:37565" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00367" FT BINDING 64..68 FT /ligand="GTP" FT /ligand_id="ChEBI:CHEBI:37565" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00367" FT BINDING 122..125 FT /ligand="GTP" FT /ligand_id="ChEBI:CHEBI:37565" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00367" SQ SEQUENCE 301 AA; 34422 MW; 92994DE2DA728BB5 CRC64; MKTKAGFVAL IGKPNAGKST LLNTLLNAHL ALVSHKANAT RKLMKCIVPF KDKEGYESQI IFLDTPGLHH QEKLLNQCML SQALKAMGDA ELCVFLASVH DDLKGYEEFL SLCQKPHILA LSKIDTATHK QVLQKLQEYQ QYDSQFLALV PLSAKKSQNL NALLECISKH LNPSAWLFEK DLMSDEKMRD IYKEIIRESL FDFLSDEIPY ESDVMIDKFI EEERIDKVYA RIIVEKESQK KIVIGKNGVN IKRIGTSARL KMQEVGEKKV FLNLQVIAQK SWSKEEKSLQ KLGYTHQRNR D //