ID ISPF_ECOSE Reviewed; 159 AA. AC B6I6D6; DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot. DT 16-DEC-2008, sequence version 1. DT 14-APR-2009, entry version 5. DE RecName: Full=2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; DE Short=MECPS; DE Short=MECDP-synthase; DE EC=4.6.1.12; GN Name=ispF; OrderedLocusNames=ECSE_2998; OS Escherichia coli (strain SE11). OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. OX NCBI_TaxID=409438; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=18931093; DOI=10.1093/dnares/dsn026; RA Oshima K., Toh H., Ogura Y., Sasamoto H., Morita H., Park S.-H., RA Ooka T., Iyoda S., Taylor T.D., Hayashi T., Itoh K., Hattori M.; RT "Complete genome sequence and comparative analysis of the wild-type RT commensal Escherichia coli strain SE11 isolated from a healthy RT adult."; RL DNA Res. 15:375-386(2008). CC -!- FUNCTION: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- CC phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate CC (MECDP) and CMP (By similarity). CC -!- CATALYTIC ACTIVITY: 2-phospho-4-(cytidine 5'-diphospho)-2-C- CC methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate CC + CMP. CC -!- COFACTOR: Binds 1 divalent metal cation per subunit (By CC similarity). CC -!- PATHWAY: Isoprenoid biosynthesis; isopentenyl-PP biosynthesis via CC DXP pathway; isopentenyl-PP from 1-deoxy-D-xylulose 5-phosphate: CC step 4/6. CC -!- SUBUNIT: Homotrimer (By similarity). CC -!- SIMILARITY: Belongs to the ispF family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AP009240; BAG78522.1; -; Genomic_DNA. DR RefSeq; YP_002294273.1; -. DR GeneID; 7001383; -. DR GenomeReviews; AP009240_GR; ECSE_2998. DR GO; GO:0008685; F:2-C-methyl-D-erythritol 2,4-cyclodiphosphat...; IEA:InterPro. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0016114; P:terpenoid biosynthetic process; IEA:InterPro. DR HAMAP; MF_00107; -; 1. DR InterPro; IPR003526; MECDP_synthase_core. DR Gene3D; G3DSA:3.30.1330.50; MECDP_synthase_core; 1. DR Pfam; PF02542; YgbB; 1. DR TIGRFAMs; TIGR00151; ispF; 1. DR PROSITE; PS01350; ISPF; 1. PE 3: Inferred from homology; KW Complete proteome; Isoprene biosynthesis; Lyase; Metal-binding. FT CHAIN 1 159 2-C-methyl-D-erythritol 2,4- FT cyclodiphosphate synthase. FT /FTId=PRO_1000094261. FT METAL 8 8 Divalent metal cation (By similarity). FT METAL 10 10 Divalent metal cation (By similarity). FT METAL 42 42 Divalent metal cation (By similarity). FT SITE 34 34 Transition state stabilizer (By FT similarity). FT SITE 133 133 Transition state stabilizer (By FT similarity). SQ SEQUENCE 159 AA; 16898 MW; 9FC5563623A62939 CRC64; MRIGHGFDVH AFGGEGPIII GGVRIPYEKG LLAHSDGDVA LHALTDALLG AAALGDIGKL FPDTDPAFKG ADSRELLREA WRRIQAKGYT LGNVDVTIIA QAPKMLPHIP QMRVFIAEDL GCHMDDVNVK ATTTEKLGFT GRGEGIACEA VALLIKATK //