ID   ISPF_ECOSE              Reviewed;         159 AA.
AC   B6I6D6;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   16-DEC-2008, sequence version 1.
DT   08-MAR-2011, entry version 22.
DE   RecName: Full=2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase;
DE            Short=MECDP-synthase;
DE            Short=MECPS;
DE            EC=4.6.1.12;
GN   Name=ispF; OrderedLocusNames=ECSE_2998;
OS   Escherichia coli (strain SE11).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=409438;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SE11;
RX   PubMed=18931093; DOI=10.1093/dnares/dsn026;
RA   Oshima K., Toh H., Ogura Y., Sasamoto H., Morita H., Park S.-H.,
RA   Ooka T., Iyoda S., Taylor T.D., Hayashi T., Itoh K., Hattori M.;
RT   "Complete genome sequence and comparative analysis of the wild-type
RT   commensal Escherichia coli strain SE11 isolated from a healthy
RT   adult.";
RL   DNA Res. 15:375-386(2008).
CC   -!- FUNCTION: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-
CC       phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate
CC       (MECDP) and CMP (By similarity).
CC   -!- CATALYTIC ACTIVITY: 2-phospho-4-(cytidine 5'-diphospho)-2-C-
CC       methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate
CC       + CMP.
CC   -!- COFACTOR: Binds 1 divalent metal cation per subunit (By
CC       similarity).
CC   -!- PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate
CC       biosynthesis via DXP pathway; isopentenyl diphosphate from 1-
CC       deoxy-D-xylulose 5-phosphate: step 4/6.
CC   -!- SUBUNIT: Homotrimer (By similarity).
CC   -!- SIMILARITY: Belongs to the ispF family.
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DR   EMBL; AP009240; BAG78522.1; -; Genomic_DNA.
DR   RefSeq; YP_002294273.1; NC_011415.1.
DR   ProteinModelPortal; B6I6D6; -.
DR   SMR; B6I6D6; 1-156.
DR   EnsemblBacteria; EBESCT00000108466; EBESCP00000099227; EBESCG00000109185.
DR   GeneID; 7001383; -.
DR   GenomeReviews; AP009240_GR; ECSE_2998.
DR   KEGG; ecy:ECSE_2998; -.
DR   GeneTree; EBGT00050000010504; -.
DR   HOGENOM; HBG630070; -.
DR   OMA; HKFGGEG; -.
DR   ProtClustDB; PRK00084; -.
DR   BioCyc; ECOL409438:ECSE_2998-MONOMER; -.
DR   GO; GO:0008685; F:2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity; IEA:EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016114; P:terpenoid biosynthetic process; IEA:InterPro.
DR   HAMAP; MF_00107; IspF; 1; -.
DR   InterPro; IPR003526; MECDP_synthase.
DR   InterPro; IPR020555; MECDP_synthase_CS.
DR   Pfam; PF02542; YgbB; 1.
DR   TIGRFAMs; TIGR00151; ispF; 1.
DR   PROSITE; PS01350; ISPF; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Isoprene biosynthesis; Lyase; Metal-binding.
FT   CHAIN         1    159       2-C-methyl-D-erythritol 2,4-
FT                                cyclodiphosphate synthase.
FT                                /FTId=PRO_1000094261.
FT   METAL         8      8       Divalent metal cation (By similarity).
FT   METAL        10     10       Divalent metal cation (By similarity).
FT   METAL        42     42       Divalent metal cation (By similarity).
FT   SITE         34     34       Transition state stabilizer (By
FT                                similarity).
FT   SITE        133    133       Transition state stabilizer (By
FT                                similarity).
SQ   SEQUENCE   159 AA;  16898 MW;  9FC5563623A62939 CRC64;
     MRIGHGFDVH AFGGEGPIII GGVRIPYEKG LLAHSDGDVA LHALTDALLG AAALGDIGKL
     FPDTDPAFKG ADSRELLREA WRRIQAKGYT LGNVDVTIIA QAPKMLPHIP QMRVFIAEDL
     GCHMDDVNVK ATTTEKLGFT GRGEGIACEA VALLIKATK
//