ID B6F0W9_9CARY Unreviewed; 268 AA. AC B6F0W9; DT 16-DEC-2008, integrated into UniProtKB/TrEMBL. DT 16-DEC-2008, sequence version 1. DT 14-DEC-2022, entry version 31. DE RecName: Full=stizolobate synthase {ECO:0000256|ARBA:ARBA00013224}; DE EC=1.13.11.29 {ECO:0000256|ARBA:ARBA00013224}; GN Name=dod {ECO:0000313|EMBL:BAG80687.1}; OS Bougainvillea glabra. OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC Caryophyllales; Nyctaginaceae; Bougainvillea. OX NCBI_TaxID=3541 {ECO:0000313|EMBL:BAG80687.1}; RN [1] {ECO:0000313|EMBL:BAG80687.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=19366710; DOI=10.1093/pcp/pcp053; RA Sasaki N., Abe Y., Goda Y., Adachi T., Kasahara K., Ozeki Y.; RT "Detection of DOPA 4,5-dioxygenase (DOD) activity using recombinant protein RT prepared from Escherichia coli cells harboring cDNA encoding DOD from RT Mirabilis jalapa."; RL Plant Cell Physiol. 50:1012-1016(2009). CC -!- CATALYTIC ACTIVITY: CC Reaction=L-dopa + O2 = 4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6- CC semialdehyde + H(+); Xref=Rhea:RHEA:21220, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:15379, ChEBI:CHEBI:57504, ChEBI:CHEBI:57639; CC EC=1.13.11.29; Evidence={ECO:0000256|ARBA:ARBA00000466}; CC -!- COFACTOR: CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; CC Evidence={ECO:0000256|ARBA:ARBA00001947}; CC -!- PATHWAY: Pigment biosynthesis; betalain biosynthesis. CC {ECO:0000256|ARBA:ARBA00005034}. CC -!- SIMILARITY: Belongs to the DODA-type extradiol aromatic ring-opening CC dioxygenase family. {ECO:0000256|ARBA:ARBA00007581}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AB435373; BAG80687.1; -; mRNA. DR AlphaFoldDB; B6F0W9; -. DR UniPathway; UPA00278; -. DR GO; GO:0008198; F:ferrous iron binding; IEA:InterPro. DR GO; GO:0050297; F:stizolobate synthase activity; IEA:UniProtKB-EC. DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro. DR GO; GO:0006725; P:cellular aromatic compound metabolic process; IEA:InterPro. DR CDD; cd07363; 45_DOPA_Dioxygenase; 1. DR InterPro; IPR014436; Extradiol_dOase_DODA. DR InterPro; IPR004183; Xdiol_dOase_suB. DR Pfam; PF02900; LigB; 1. DR PIRSF; PIRSF006157; Doxgns_DODA; 1. PE 2: Evidence at transcript level; KW Dioxygenase {ECO:0000313|EMBL:BAG80687.1}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723}; KW Oxidoreductase {ECO:0000313|EMBL:BAG80687.1}; KW Zinc {ECO:0000256|ARBA:ARBA00022833}. FT DOMAIN 10..261 FT /note="LigB" FT /evidence="ECO:0000259|Pfam:PF02900" SQ SEQUENCE 268 AA; 30042 MW; 1FFB19053AB3EF02 CRC64; MGGEKKMKGT YYIAHGDPIM YINKSIKLRH FLEEWKENVV MEKPICILVI SAHWDTDVPT VNLVEHCDTI HDFDDYPDPL YQIKYPAPGA PKLAMKVQEL LKGGGFKCEV DTKRGLDHAA WFPLMLMYPE ADIPICELSV QTNKDGTHHY NLGKALSPLL NDDVLIIGSG GAVHPSDDTP HCPNGVAPWA LQFDNWLEDA LLSGRYEDVK EFKKMAPNWE ISHPGQEHLY PLHVALGAAG NNVKTELIHQ TWAANGVFGY SSYKFTST //