ID KDUI_ECO5E Reviewed; 278 AA. AC B5Z4G1; DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot. DT 25-NOV-2008, sequence version 1. DT 03-OCT-2012, entry version 33. DE RecName: Full=4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase; DE EC=5.3.1.17; DE AltName: Full=5-keto-4-deoxyuronate isomerase; DE AltName: Full=DKI isomerase; GN Name=kduI; OrderedLocusNames=ECH74115_4112; OS Escherichia coli O157:H7 (strain EC4115 / EHEC). OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. OX NCBI_TaxID=444450; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=EC4115 / EHEC; RA Eppinger M., Sebastian Y., Ravel J.; RT "Complete genome sequence of Escherichia coli O157:H7 str. EC4115."; RL Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Catalyzes the isomerization of 5-dehydro-4-deoxy-D- CC glucuronate to 3-deoxy-D-glycero-2,5-hexodiulosonate (By CC similarity). CC -!- CATALYTIC ACTIVITY: 5-dehydro-4-deoxy-D-glucuronate = 3-deoxy-D- CC glycero-2,5-hexodiulosonate. CC -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity). CC -!- PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy- CC D-gluconate from pectin: step 4/5. CC -!- SUBUNIT: Homohexamer (By similarity). CC -!- SIMILARITY: Belongs to the KduI family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP001164; ACI38163.1; -; Genomic_DNA. DR RefSeq; YP_002272307.1; NC_011353.1. DR ProteinModelPortal; B5Z4G1; -. DR SMR; B5Z4G1; 1-277. DR STRING; B5Z4G1; -. DR EnsemblBacteria; EBESCT00000030932; EBESCP00000029635; EBESCG00000029982. DR GeneID; 6971923; -. DR GenomeReviews; CP001164_GR; ECH74115_4112. DR KEGG; ecf:ECH74115_4112; -. DR PATRIC; 18368837; VBIEscCol74651_4046. DR eggNOG; COG3717; -. DR HOGENOM; HOG000124379; -. DR KO; K01815; -. DR OMA; AKFYLVS; -. DR ProtClustDB; PRK00924; -. DR UniPathway; UPA00545; UER00826. DR GO; GO:0008697; F:4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase activity; IEA:EC. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0045490; P:pectin catabolic process; IEA:InterPro. DR HAMAP; MF_00687; KduI; 1; -. DR InterPro; IPR007045; KduI. DR InterPro; IPR021120; KduI/IolB_isomerase. DR InterPro; IPR011051; RmlC_Cupin. DR Pfam; PF04962; KduI; 1. DR PIRSF; PIRSF006625; KduI; 1. DR SUPFAM; SSF51182; RmlC_like_cupin; 1. PE 3: Inferred from homology; KW Complete proteome; Isomerase; Metal-binding; Zinc. FT CHAIN 1 278 4-deoxy-L-threo-5-hexosulose-uronate FT ketol-isomerase. FT /FTId=PRO_1000131879. FT METAL 196 196 Zinc (By similarity). FT METAL 198 198 Zinc (By similarity). FT METAL 203 203 Zinc (By similarity). FT METAL 245 245 Zinc (By similarity). SQ SEQUENCE 278 AA; 31090 MW; B8E4098A31BEB6A2 CRC64; MDVRQSIHSA HAKTLETQGL RNEFLVEKVF VADEYTMVYS HIDRIIIGGI MPVTKTVSVG GEVGKQLGVS YFLERRELGV INIGGAGTIT VDGQCYEIGH RDALYVGKGA KEVVFASIDT GTPAKFYYNC APAHTTYPTK KVTPDEVSPV TLGDNLTSNR RTINKYFVPD VLETCQLSMG LTELAPGNLW NTMPCHTHER RMEVYFYFNM DDDACVFHMM GQPQETRHIV MHNEQAVISP SWSIHSGVGT KAYTFIWGMV GENQVFDDMD HVAVKDLR //