ID B5MGR3_PENCH Unreviewed; 352 AA. AC B5MGR3; DT 04-NOV-2008, integrated into UniProtKB/TrEMBL. DT 04-NOV-2008, sequence version 1. DT 24-JUL-2024, entry version 50. DE RecName: Full=Arabinogalactan endo-beta-1,4-galactanase {ECO:0000256|ARBA:ARBA00012556, ECO:0000256|RuleBase:RU361192}; DE EC=3.2.1.89 {ECO:0000256|ARBA:ARBA00012556, ECO:0000256|RuleBase:RU361192}; GN Name=gal1 {ECO:0000313|EMBL:BAG71683.1}; GN Synonyms=pc {ECO:0000313|EMBL:BAG71683.1}; OS Penicillium chrysogenum (Penicillium notatum). OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes; OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium; OC Penicillium chrysogenum species complex. OX NCBI_TaxID=5076 {ECO:0000313|EMBL:BAG71683.1}; RN [1] {ECO:0000313|EMBL:BAG71683.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=31B {ECO:0000313|EMBL:BAG71683.1}; RA Sakamoto T., Nishimura Y., Makino Y., Sunagawa Y., Harada N.; RT "Biochemical characterization of a GH53 endo-beta-1,4-galactanase and a RT GH35 exo-beta-1,4-galactanase from Penicillium chrysogenum."; RL Appl. Microbiol. Biotechnol. 9:2895-2906(2013). CC -!- CATALYTIC ACTIVITY: CC Reaction=The enzyme specifically hydrolyzes (1->4)-beta-D-galactosidic CC linkages in type I arabinogalactans.; EC=3.2.1.89; CC Evidence={ECO:0000256|ARBA:ARBA00001695, CC ECO:0000256|RuleBase:RU361192}; CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 53 family. CC {ECO:0000256|ARBA:ARBA00010687, ECO:0000256|RuleBase:RU361192}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AB461445; BAG71683.1; -; mRNA. DR AlphaFoldDB; B5MGR3; -. DR SMR; B5MGR3; -. DR CAZy; GH53; Glycoside Hydrolase Family 53. DR BRENDA; 3.2.1.89; 4606. DR GO; GO:0031218; F:arabinogalactan endo-1,4-beta-galactosidase activity; IEA:UniProtKB-EC. DR GO; GO:0015926; F:glucosidase activity; IEA:InterPro. DR GO; GO:0045490; P:pectin catabolic process; IEA:TreeGrafter. DR Gene3D; 3.20.20.80; Glycosidases; 1. DR InterPro; IPR011683; Glyco_hydro_53. DR InterPro; IPR017853; Glycoside_hydrolase_SF. DR PANTHER; PTHR34983; ARABINOGALACTAN ENDO-BETA-1,4-GALACTANASE A; 1. DR PANTHER; PTHR34983:SF1; ARABINOGALACTAN ENDO-BETA-1,4-GALACTANASE A; 1. DR Pfam; PF07745; Glyco_hydro_53; 1. DR SUPFAM; SSF51445; (Trans)glycosidases; 1. PE 2: Evidence at transcript level; KW Glycosidase {ECO:0000256|RuleBase:RU361192, ECO:0000313|EMBL:BAG71683.1}; KW Hydrolase {ECO:0000256|RuleBase:RU361192, ECO:0000313|EMBL:BAG71683.1}; KW Signal {ECO:0000256|RuleBase:RU361192}. FT SIGNAL 1..18 FT /evidence="ECO:0000256|RuleBase:RU361192" FT CHAIN 19..352 FT /note="Arabinogalactan endo-beta-1,4-galactanase" FT /evidence="ECO:0000256|RuleBase:RU361192" FT /id="PRO_5005123489" SQ SEQUENCE 352 AA; 38368 MW; 7F6F582417836FD9 CRC64; MLPHLLLAAA QLFASSSAAL TYNGADISSL LVEEGKGVSY KNLAGTPEKL ETILSASGVN SVRQRIWVNP SDGSYDLDYN VKLAKRVQAQ GMGTYLDLHY SDTWADPKSQ TTPSGWSTTD IGILAGQVYD YTLDVCNTFA ANKIDVDIVS IGNEIRNGLL WPLGGTSNYN NIARLLHSAA WGVKDSKLAT TPKIMIHLDN GWDSGAQSYF YDQVLAPGSG LVSTDFDYIG VSYYPFYNAD ATLAALKTSL TNLHSKYKKE TLVVETNWPF SCPNPEYAFP TDLKDIPFSV EGQQTFLQRL AKAVEEVGGL GIYYWEPAWV DNAGLGSSCD DNLFFAWSND QARASLDTLG GL //