ID NUDC_SALHS Reviewed; 257 AA. AC B4TCT5; DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot. DT 23-SEP-2008, sequence version 1. DT 11-NOV-2015, entry version 48. DE RecName: Full=NADH pyrophosphatase {ECO:0000255|HAMAP-Rule:MF_00297}; DE EC=3.6.1.22 {ECO:0000255|HAMAP-Rule:MF_00297}; GN Name=nudC {ECO:0000255|HAMAP-Rule:MF_00297}; GN OrderedLocusNames=SeHA_C4497; OS Salmonella heidelberg (strain SL476). OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Salmonella. OX NCBI_TaxID=454169; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=SL476; RX PubMed=21602358; DOI=10.1128/JB.00297-11; RA Fricke W.F., Mammel M.K., McDermott P.F., Tartera C., White D.G., RA Leclerc J.E., Ravel J., Cebula T.A.; RT "Comparative genomics of 28 Salmonella enterica isolates: evidence for RT CRISPR-mediated adaptive sublineage evolution."; RL J. Bacteriol. 193:3556-3568(2011). CC -!- CATALYTIC ACTIVITY: NAD(+) + H(2)O = AMP + NMN. CC {ECO:0000255|HAMAP-Rule:MF_00297}. CC -!- COFACTOR: CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; CC Evidence={ECO:0000255|HAMAP-Rule:MF_00297}; CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; CC Evidence={ECO:0000255|HAMAP-Rule:MF_00297}; CC Note=Divalent metal cations. Mg(2+) or Mn(2+). {ECO:0000255|HAMAP- CC Rule:MF_00297}; CC -!- COFACTOR: CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; CC Evidence={ECO:0000255|HAMAP-Rule:MF_00297}; CC Note=Binds 1 zinc ion per subunit. {ECO:0000255|HAMAP- CC Rule:MF_00297}; CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00297}. CC -!- SIMILARITY: Belongs to the Nudix hydrolase family. NudC subfamily. CC {ECO:0000255|HAMAP-Rule:MF_00297}. CC -!- SIMILARITY: Contains 1 nudix hydrolase domain. {ECO:0000255|HAMAP- CC Rule:MF_00297}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP001120; ACF66801.1; -; Genomic_DNA. DR RefSeq; WP_000373960.1; NC_011083.1. DR ProteinModelPortal; B4TCT5; -. DR EnsemblBacteria; ACF66801; ACF66801; SeHA_C4497. DR KEGG; seh:SeHA_C4497; -. DR PATRIC; 18516431; VBISalEnt43179_4260. DR HOGENOM; HOG000247937; -. DR KO; K03426; -. DR OMA; DHGWWIV; -. DR OrthoDB; EOG6S26JD; -. DR BioCyc; SENT454169:GHYG-4466-MONOMER; -. DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-HAMAP. DR GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-HAMAP. DR GO; GO:0000210; F:NAD+ diphosphatase activity; IEA:UniProtKB-HAMAP. DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-HAMAP. DR Gene3D; 3.90.79.10; -; 1. DR HAMAP; MF_00297; Nudix_NudC; 1. DR InterPro; IPR022925; NADH_pyroPase_NudC. DR InterPro; IPR020084; NUDIX_hydrolase_CS. DR InterPro; IPR000086; NUDIX_hydrolase_dom. DR InterPro; IPR015797; NUDIX_hydrolase_dom-like. DR InterPro; IPR015376; Znr_NADH_PPase. DR Pfam; PF00293; NUDIX; 1. DR Pfam; PF09297; zf-NADH-PPase; 1. DR SUPFAM; SSF55811; SSF55811; 2. DR PROSITE; PS51462; NUDIX; 1. DR PROSITE; PS00893; NUDIX_BOX; 1. PE 3: Inferred from homology; KW Hydrolase; Magnesium; Manganese; Metal-binding; NAD; Zinc. FT CHAIN 1 257 NADH pyrophosphatase. FT /FTId=PRO_1000115250. FT DOMAIN 125 248 Nudix hydrolase. {ECO:0000255|HAMAP- FT Rule:MF_00297}. FT MOTIF 159 180 Nudix box. FT METAL 98 98 Zinc. {ECO:0000255|HAMAP-Rule:MF_00297}. FT METAL 101 101 Zinc. {ECO:0000255|HAMAP-Rule:MF_00297}. FT METAL 116 116 Zinc. {ECO:0000255|HAMAP-Rule:MF_00297}. FT METAL 119 119 Zinc. {ECO:0000255|HAMAP-Rule:MF_00297}. FT METAL 174 174 Divalent metal cation. FT {ECO:0000255|HAMAP-Rule:MF_00297}. FT METAL 178 178 Divalent metal cation. FT {ECO:0000255|HAMAP-Rule:MF_00297}. SQ SEQUENCE 257 AA; 29635 MW; D8F0B689D0F4555C CRC64; MDRIIEKLES GWWIVSHEQK LWLPYGELPH GLAANFDLVG QRALRIGEWQ GEPVWLVLQH RRHDMGSVRQ VIDQDAGLFQ LAGRGVQLAE FYRSHKFCGY CGHPMHPSKT EWAMLCSHCR ERYYPQIAPC IIVAIRREDS ILLARHVRHR NGVHTVLAGF VEVGETLEQA VAREVMEESG IKVKNLRYVT SQPWPFPQSL MTAFMAEYDS GEIVIDPKEL LEANWYRYDD LPLLPPPGTV ARRLIEDTVA MCRAEYD //