ID B4DNG5_HUMAN Unreviewed; 175 AA. AC B4DNG5; DT 23-SEP-2008, integrated into UniProtKB/TrEMBL. DT 23-SEP-2008, sequence version 1. DT 14-DEC-2022, entry version 49. DE RecName: Full=3'-phosphate/5'-hydroxy nucleic acid ligase {ECO:0000256|ARBA:ARBA00012726}; DE EC=6.5.1.8 {ECO:0000256|ARBA:ARBA00012726}; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; OC Homo. OX NCBI_TaxID=9606 {ECO:0000313|EMBL:BAG60227.1}; RN [1] {ECO:0000313|EMBL:BAG60227.1} RP NUCLEOTIDE SEQUENCE. RC TISSUE=Whole embryo {ECO:0000313|EMBL:BAG60227.1}; RA Wakamatsu A., Yamamoto J., Kimura K., Ishii S., Watanabe K., Sugiyama A., RA Murakawa K., Kaida T., Tsuchiya K., Fukuzumi Y., Kumagai A., Oishi Y., RA Yamamoto S., Ono Y., Komori Y., Yamazaki M., Kisu Y., Nishikawa T., RA Sugano S., Nomura N., Isogai T.; RT "NEDO human cDNA sequencing project focused on splicing variants."; RL Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + a 5'-end CC dephospho-ribonucleoside-RNA + GTP + H2O = a ribonucleotidyl- CC ribonucleotide-RNA + diphosphate + GMP + H(+); Xref=Rhea:RHEA:68080, CC Rhea:RHEA-COMP:10464, Rhea:RHEA-COMP:13936, Rhea:RHEA-COMP:17355, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, CC ChEBI:CHEBI:37565, ChEBI:CHEBI:58115, ChEBI:CHEBI:83064, CC ChEBI:CHEBI:138284, ChEBI:CHEBI:173118; EC=6.5.1.8; CC Evidence={ECO:0000256|ARBA:ARBA00029282}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho- CC ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA + CC diphosphate + GMP; Xref=Rhea:RHEA:68076, Rhea:RHEA-COMP:10463, CC Rhea:RHEA-COMP:13936, Rhea:RHEA-COMP:17355, ChEBI:CHEBI:33019, CC ChEBI:CHEBI:37565, ChEBI:CHEBI:58115, ChEBI:CHEBI:83062, CC ChEBI:CHEBI:138284, ChEBI:CHEBI:173118; EC=6.5.1.8; CC Evidence={ECO:0000256|ARBA:ARBA00029311}; CC -!- COFACTOR: CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; CC Evidence={ECO:0000256|PIRSR:PIRSR601233-3}; CC Note=Binds 2 manganese ions per subunit. CC {ECO:0000256|PIRSR:PIRSR601233-3}; CC -!- SIMILARITY: Belongs to the RtcB family. CC {ECO:0000256|ARBA:ARBA00008071}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AK297908; BAG60227.1; -; mRNA. DR AlphaFoldDB; B4DNG5; -. DR MaxQB; B4DNG5; -. DR PeptideAtlas; B4DNG5; -. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0008452; F:RNA ligase activity; IEA:InterPro. DR GO; GO:0006396; P:RNA processing; IEA:InterPro. DR Gene3D; 3.90.1860.10; -; 1. DR InterPro; IPR001233; RtcB. DR InterPro; IPR036025; RtcB-like_sf. DR PANTHER; PTHR11118; UNCHARACTERIZED; 1. DR Pfam; PF01139; RtcB; 1. DR SUPFAM; SSF103365; Hypothetical protein PH1602; 1. DR PROSITE; PS01288; UPF0027; 1. PE 2: Evidence at transcript level; KW GTP-binding {ECO:0000256|ARBA:ARBA00023134}; KW Ligase {ECO:0000256|ARBA:ARBA00022598}; KW Manganese {ECO:0000256|ARBA:ARBA00023211, ECO:0000256|PIRSR:PIRSR601233-3}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, KW ECO:0000256|PIRSR:PIRSR601233-3}; KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741}; KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}. FT BINDING 119 FT /ligand="Mn(2+)" FT /ligand_id="ChEBI:CHEBI:29035" FT /ligand_label="1" FT /evidence="ECO:0000256|PIRSR:PIRSR601233-3" SQ SEQUENCE 175 AA; 19093 MW; 79D107FDF06000B2 CRC64; MSRSYNDELQ FLEKINKNCW RIKKGFVPNM QVEGVFYVND ALEKLMFEEL RNACRGGGVG GFLPAMKQIG NVAALPGIVH RSIGLPDVHS GYGFAIGNMA AFDMNDPEAV VSPGGVGFDI NCGVRLLRTN LDESDVQPVK EQLAQAMFDH IPVGVGSKGV MERAVHCPEQ NLDVI //