ID B3KUV5_HUMAN Unreviewed; 315 AA. AC B3KUV5; DT 02-SEP-2008, integrated into UniProtKB/TrEMBL. DT 02-SEP-2008, sequence version 1. DT 02-OCT-2024, entry version 70. DE RecName: Full=ubiquitinyl hydrolase 1 {ECO:0000256|ARBA:ARBA00012759}; DE EC=3.4.19.12 {ECO:0000256|ARBA:ARBA00012759}; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; OC Homo. OX NCBI_TaxID=9606 {ECO:0000313|EMBL:BAG53567.1}; RN [1] {ECO:0000313|EMBL:BAG53567.1} RP NUCLEOTIDE SEQUENCE. RC TISSUE=Thymus {ECO:0000313|EMBL:BAG53567.1}; RX PubMed=14702039; DOI=10.1038/ng1285; RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., RA Tanase T., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., RA Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., RA Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., RA Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., RA Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., RA Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., RA Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., RA Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., RA Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., RA Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., RA Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., RA Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., RA Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., RA Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., RA Sugano S.; RT "Complete sequencing and characterization of 21,243 full-length human RT cDNAs."; RL Nat. Genet. 36:40-45(2004). CC -!- CATALYTIC ACTIVITY: CC Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide CC and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76- CC residue protein attached to proteins as an intracellular targeting CC signal).; EC=3.4.19.12; Evidence={ECO:0000256|ARBA:ARBA00000707}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AK098029; BAG53567.1; -; mRNA. DR RefSeq; NP_060140.2; NM_017670.2. DR AlphaFoldDB; B3KUV5; -. DR MEROPS; C65.001; -. DR PeptideAtlas; B3KUV5; -. DR DNASU; 55611; -. DR GeneID; 55611; -. DR KEGG; hsa:55611; -. DR CTD; 55611; -. DR DisGeNET; 55611; -. DR PharmGKB; PA134988141; -. DR OrthoDB; 148019at2759; -. DR BioGRID-ORCS; 55611; 53 hits in 1170 CRISPR screens. DR GO; GO:0005634; C:nucleus; IEA:TreeGrafter. DR GO; GO:0004843; F:cysteine-type deubiquitinase activity; IEA:UniProtKB-EC. DR GO; GO:0043130; F:ubiquitin binding; IEA:TreeGrafter. DR GO; GO:2000780; P:negative regulation of double-strand break repair; IEA:TreeGrafter. DR GO; GO:0071108; P:protein K48-linked deubiquitination; IEA:TreeGrafter. DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW. DR Gene3D; 3.30.200.60; Peptidase C65 Otubain, subdomain 1; 1. DR Gene3D; 1.20.1300.20; Peptidase C65 Otubain, subdomain 2; 1. DR InterPro; IPR003323; OTU_dom. DR InterPro; IPR038765; Papain-like_cys_pep_sf. DR InterPro; IPR019400; Peptidase_C65_otubain. DR InterPro; IPR042468; Peptidase_C65_otubain_sub1. DR InterPro; IPR042467; Peptidase_C65_otubain_sub2. DR PANTHER; PTHR12931:SF19; UBIQUITIN THIOESTERASE OTUB1; 1. DR PANTHER; PTHR12931; UBIQUITIN THIOLESTERASE PROTEIN OTUB; 1. DR Pfam; PF10275; Peptidase_C65; 1. DR SUPFAM; SSF54001; Cysteine proteinases; 1. DR PROSITE; PS50802; OTU; 1. PE 2: Evidence at transcript level; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801}; KW Protease {ECO:0000256|ARBA:ARBA00022670}; KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786}. FT DOMAIN 203..315 FT /note="OTU" FT /evidence="ECO:0000259|PROSITE:PS50802" SQ SEQUENCE 315 AA; 35313 MW; 1967EB8F1B28868E CRC64; MMKPSWLSRT EFSKRLLCRT LWCQSGWSSR SYTRSMLKMT TSINRRSRTS TKSTRTSARP GLTATVSIGL SDSPTWRHCW MTARSCSGEK GGHWAPRQVG VYLLPGRVGC VSSRVSPSFP GDGLDSGLAR RGSAVSALAS GLVEEPMLGP PFHPTPRFKA VSAKSKEDLV SQGFTEFTIE DFHNTFMDLI EQVEKQTSVA DLLASFNDQS TSDYLVVYLR LLTSGYLQRE SKFFEHFIEG GRTVKEFCQQ EVEPMCKESD HIHIIALAQV LSVSIQVEYM DRGEGGTTNP HIFPEGSEPK VYLLYRPGHY DILYK //