ID B3DWU2_METI4 Unreviewed; 689 AA. AC B3DWU2; DT 22-JUL-2008, integrated into UniProtKB/TrEMBL. DT 22-JUL-2008, sequence version 1. DT 10-FEB-2009, entry version 7. DE SubName: Full=NAD-dependent DNA ligase; GN Name=lig; OrderedLocusNames=Minf_1701; OS Methylacidiphilum infernorum (isolate V4) (Methylokorus infernorum OS (strain V4)). OC Bacteria; Verrucomicrobia; unclassified Verrucomicrobia; OC Methylacidiphilum. OX NCBI_TaxID=481448; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=18593465; DOI=10.1186/1745-6150-3-26; RA Hou S., Makarova K.S., Saw J.H., Senin P., Ly B.V., Zhou Z., Ren Y., RA Wang J., Galperin M.Y., Omelchenko M.V., Wolf Y.I., Yutin N., RA Koonin E.V., Stott M.B., Mountain B.W., Crowe M.A., Smirnova A.V., RA Dunfield P.F., Feng L., Wang L., Alam M.; RT "Complete genome sequence of the extremely acidophilic methanotroph RT isolate V4, Methylacidiphilum infernorum, a representative of the RT bacterial phylum Verrucomicrobia."; RL Biol. Direct 3:26-26(2008). CC -!- FUNCTION: This protein catalyzes the formation of phosphodiester CC linkages between 5'-phosphoryl and 3'-hydroxyl groups in double- CC stranded DNA using NAD as a coenzyme and as the energy source for CC the reaction. It is essential for DNA replication and repair of CC damaged DNA. CC -!- CATALYTIC ACTIVITY: NAD(+) + (deoxyribonucleotide)(n) + CC (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + CC (deoxyribonucleotide)(n+m). CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP000975; ACD83755.1; -; Genomic_DNA. DR RefSeq; YP_001940353.1; -. DR GeneID; 6352646; -. DR GenomeReviews; CP000975_GR; Minf_1701. DR KEGG; min:Minf_1701; -. DR GO; GO:0005622; C:intracellular; IEA:InterPro. DR GO; GO:0003911; F:DNA ligase (NAD+) activity; IEA:InterPro. DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro. DR GO; GO:0006281; P:DNA repair; IEA:InterPro. DR GO; GO:0006260; P:DNA replication; IEA:InterPro. DR InterPro; IPR001357; BRCT. DR InterPro; IPR004150; DNA_ligase_OB. DR InterPro; IPR001679; DNAligase. DR InterPro; IPR013839; DNAligase_adenylation. DR InterPro; IPR013840; DNAligase_N. DR InterPro; IPR003583; HHH1_bd. DR InterPro; IPR012340; NA-bd_OB-fold. DR InterPro; IPR004149; Znf_DNAligase_C4. DR Gene3D; G3DSA:2.40.50.140; OB_NA_bd_sub; 1. DR Pfam; PF00533; BRCT; 1. DR Pfam; PF01653; DNA_ligase_aden; 1. DR Pfam; PF03120; DNA_ligase_OB; 1. DR Pfam; PF03119; DNA_ligase_ZBD; 1. DR PIRSF; PIRSF001604; LigA; 1. DR SMART; SM00292; BRCT; 1. DR SMART; SM00278; HhH1; 3. DR SMART; SM00532; LIGANc; 1. DR TIGRFAMs; TIGR00575; dnlj; 1. DR PROSITE; PS50172; BRCT; 1. DR PROSITE; PS01055; DNA_LIGASE_N1; 1. DR PROSITE; PS01056; DNA_LIGASE_N2; 1. PE 4: Predicted; KW Complete proteome; DNA damage; DNA repair; DNA replication; Ligase; KW NAD. SQ SEQUENCE 689 AA; 77418 MW; A067A22CB46CB6DE CRC64; MIESYLAFIF LNKVNKKKAP SLDSLGYEEI KKRHDELVKL IRKYDYAYYV EAHPLVSDQE YDNLYHELET LEKLHPELIT PDSPTQRIGE TPLSGFSQVT HEIPMLSLEN TYSKEELFAF LERIKRALPG KKISFTVEPK IDGVSISAVY KNGLFSLGAT RGNGTVGDDI TQNLKTIRSL PLRLETPDPP EYLEVRGEAY MSPKDFERLN AQREKEGKVL FANPRNATAG SLKQLDPRVV AERPLAVVFY GAGKLVGMKC KTQEEWLNFL KKIGLPIPIV FWVCSNENEV YEAIGKLNEC RNQLPYPTDG AAVKVNEWEY YSLLGYTAKA PRWAFAYKYG AERAKTRLNN VIFQVGRSGT ITPVAEMDPV FLSGTTVSRA TLHNFDQVKR LDVKIGDVVY LEKAGEVIPE VVGVDLNQRR GTEKEIVPPE FCPSCGEKLS WEGIFLRCEN ENCPAQLKER ILHFAQRNAM DIQGLGESLV DQLVDKGIVK DVADIYDLDE ETLVNLDRMG KKSAQNLLKA IEESKKKDLS RLIFGLGIPH IGQKASEDLA RYFGTMDKLS HATEEELLNL PFIGEIMARS IVNYFRKEAN RRRLEKLRKK GLNFVSTLSQ ASSGTLSGKT FVITGTLSEP RESIAQKIIS KGGRVSNSLS RKTSYLVVGS DPGSKLQEAK KLGIPLINEQ ELLEMLHGG //