ID S22AD_RAT Reviewed; 551 AA. AC B2GV36; DT 12-OCT-2022, integrated into UniProtKB/Swiss-Prot. DT 10-JUN-2008, sequence version 1. DT 27-NOV-2024, entry version 115. DE RecName: Full=Solute carrier family 22 member 13 {ECO:0000250|UniProtKB:Q9Y226}; DE AltName: Full=Organic anion transporter 10 {ECO:0000303|PubMed:35144162}; DE Short=OAT10 {ECO:0000303|PubMed:35144162}; DE AltName: Full=Organic cation transporter-like 3 {ECO:0000250|UniProtKB:Q9Y226}; DE Short=ORCTL-3 {ECO:0000250|UniProtKB:Q9Y226}; GN Name=Slc22a13 {ECO:0000312|RGD:1306840}; OS Rattus norvegicus (Rat). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Rattus. OX NCBI_TaxID=10116 {ECO:0000312|EMBL:AAI66511.1}; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Kidney; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA project: RT the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Brown Norway; RX PubMed=15057822; DOI=10.1038/nature02426; RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., RA Mockrin S., Collins F.S.; RT "Genome sequence of the Brown Norway rat yields insights into mammalian RT evolution."; RL Nature 428:493-521(2004). RN [3] RP FUNCTION, AND TRANSPORTER ACTIVITY. RX PubMed=35144162; DOI=10.1016/j.dmpk.2021.100443; RA Shinoda Y., Yamashiro T., Hosooka A., Yasujima T., Yuasa H.; RT "Functional characterization of human organic anion transporter 10 RT (OAT10/SLC22A13) as an orotate transporter."; RL Drug Metab. Pharmacokinet. 43:100443-100443(2022). CC -!- FUNCTION: Anion antiporter that mediates the transport of nicotinate CC (PubMed:35144162). Possibly involved in nicotinate intestinal CC reabsorption (By similarity). {ECO:0000250|UniProtKB:Q9Y226, CC ECO:0000269|PubMed:35144162}. CC -!- CATALYTIC ACTIVITY: CC Reaction=nicotinate(in) + a carboxylate(out) = nicotinate(out) + a CC carboxylate(in); Xref=Rhea:RHEA:74371, ChEBI:CHEBI:29067, CC ChEBI:CHEBI:32544; Evidence={ECO:0000305|PubMed:35144162}; CC -!- SUBCELLULAR LOCATION: Apical cell membrane CC {ECO:0000250|UniProtKB:Q9Y226}; Multi-pass membrane protein CC {ECO:0000250|UniProtKB:Q9Y226}. CC -!- PTM: Glycosylated. {ECO:0000250|UniProtKB:Q9Y226}. CC -!- SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily. CC Organic cation transporter (TC 2.A.1.19) family. {ECO:0000305}. CC -!- CAUTION: In contrast with human ortholog, not able to transport urate CC and orotate (PubMed:35144162). {ECO:0000269|PubMed:35144162}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; BC166511; AAI66511.1; -; mRNA. DR RefSeq; NP_001119757.1; NM_001126285.1. DR RefSeq; XP_008764899.1; XM_008766677.2. DR AlphaFoldDB; B2GV36; -. DR SMR; B2GV36; -. DR STRING; 10116.ENSRNOP00000075628; -. DR PhosphoSitePlus; B2GV36; -. DR PaxDb; 10116-ENSRNOP00000048781; -. DR Ensembl; ENSRNOT00000078303.2; ENSRNOP00000075628.1; ENSRNOG00000056476.2. DR Ensembl; ENSRNOT00000099503.1; ENSRNOP00000076627.1; ENSRNOG00000056476.2. DR Ensembl; ENSRNOT00065006783; ENSRNOP00065004667; ENSRNOG00065004676. DR GeneID; 316062; -. DR KEGG; rno:316062; -. DR UCSC; RGD:1306840; rat. DR AGR; RGD:1306840; -. DR CTD; 9390; -. DR RGD; 1306840; Slc22a13. DR eggNOG; KOG0255; Eukaryota. DR GeneTree; ENSGT00940000154607; -. DR HOGENOM; CLU_001265_33_3_1; -. DR InParanoid; B2GV36; -. DR OrthoDB; 2088942at2759; -. DR TreeFam; TF315847; -. DR PRO; PR:B2GV36; -. DR Proteomes; UP000002494; Chromosome 8. DR Bgee; ENSRNOG00000056476; Expressed in adult mammalian kidney and 5 other cell types or tissues. DR GO; GO:0016324; C:apical plasma membrane; ISO:RGD. DR GO; GO:0005783; C:endoplasmic reticulum; ISO:RGD. DR GO; GO:0005794; C:Golgi apparatus; ISO:RGD. DR GO; GO:0005886; C:plasma membrane; ISO:RGD. DR GO; GO:0090416; F:nicotinate transmembrane transporter activity; ISO:RGD. DR GO; GO:0015143; F:urate transmembrane transporter activity; ISO:RGD. DR GO; GO:0045922; P:negative regulation of fatty acid metabolic process; ISO:RGD. DR GO; GO:2001142; P:nicotinate transport; ISO:RGD. DR GO; GO:0002854; P:positive regulation of T cell mediated cytotoxicity directed against tumor cell target; ISO:RGD. DR GO; GO:0015747; P:urate transport; ISO:RGD. DR CDD; cd17374; MFS_OAT; 1. DR Gene3D; 1.20.1250.20; MFS general substrate transporter like domains; 1. DR InterPro; IPR020846; MFS_dom. DR InterPro; IPR005828; MFS_sugar_transport-like. DR InterPro; IPR036259; MFS_trans_sf. DR InterPro; IPR005829; Sugar_transporter_CS. DR PANTHER; PTHR24064; SOLUTE CARRIER FAMILY 22 MEMBER; 1. DR PANTHER; PTHR24064:SF198; SOLUTE CARRIER FAMILY 22 MEMBER 13; 1. DR Pfam; PF00083; Sugar_tr; 1. DR SUPFAM; SSF103473; MFS general substrate transporter; 1. DR PROSITE; PS50850; MFS; 1. DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 1. PE 2: Evidence at transcript level; KW Cell membrane; Membrane; Reference proteome; Transmembrane; KW Transmembrane helix. FT CHAIN 1..551 FT /note="Solute carrier family 22 member 13" FT /id="PRO_0000456464" FT TRANSMEM 23..43 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 143..163 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 168..188 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 196..216 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 227..247 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 252..272 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 333..353 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 355..375 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 394..412 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 417..439 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 453..473 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 479..499 FT /note="Helical" FT /evidence="ECO:0000255" FT REGION 515..537 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" SQ SEQUENCE 551 AA; 60691 MW; 0A0EDFF2BF2DA3CC CRC64; MAQFAQVMAE VGDFGRFQVR LTILMGIPNF LSAFFVFSQV FMVLDEAHYC SVSWVKNHTF NLSAAEQLAV SIPNDTAGRP ESCLMFRPPP DNASLEDILS HRFNETQACD SGWDYPENRP PSLKNEFDLV CERENLKRTS QSVFMAGLLV GALVFGPVCD WIGRRPSLLM QLLLSGIASM ATAFVSSFEL YMTLRFALAT ANAGFLLSSN VLLSEWVGTS RRTRAMVLAQ SNFALGLMAL AGLAYGVRNW RLLQIIGTTP IFLLVFYFWV LPESPRWLLS QGRTEEAKQL VQKAALVNGR PLASELLNQL VPEKTGPSGN ALDLFRHPQL RKVTLILIAV WFVDSLLYYG LGFQVGDFGL DIYLTQVIFG VVEVPARLSS IPMMEKLGRK WSQLCTLTLA GVMCVIIIFI PGDLPTVATA LAVVGKFASS AAFTISYVYT AELLPTIIRQ TGMGLVSIFS RVGGIITPLV MLLEQYHKAI PMLIFGSLPI GAGLLCALLP ETRGQTLKDT LQDLEQGQPP RSYKTAPQRK DVEASGRTSS ARVAVVRSSY F //