ID   B2GKG9_KOCRD            Unreviewed;       313 AA.
AC   B2GKG9;
DT   10-JUN-2008, integrated into UniProtKB/TrEMBL.
DT   10-JUN-2008, sequence version 1.
DT   16-DEC-2008, entry version 6.
DE   SubName: Full=tRNA delta(2)-isopentenylpyrophosphate transferase;
DE            EC=2.5.1.8;
GN   Name=miaA; OrderedLocusNames=KRH_15810;
OS   Kocuria rhizophila (strain ATCC 9341 / DSM 348 / NBRC 12708 / DC2201)
OS   (Micrococcus luteus).
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Micrococcineae; Micrococcaceae; Kocuria.
OX   NCBI_TaxID=378753;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=18408034; DOI=10.1128/JB.01853-07;
RA   Takarada H., Sekine M., Kosugi H., Matsuo Y., Fujisawa T., Omata S.,
RA   Kishi E., Shimizu A., Tsukatani N., Tanikawa S., Fujita N.,
RA   Harayama S.;
RT   "Complete genome sequence of the soil actinomycete Kocuria
RT   rhizophila.";
RL   J. Bacteriol. 190:4139-4146(2008).
CC   -!- FUNCTION: Catalyzes the first step in the biosynthesis of 2-
CC       methylthio-N6-(delta(2)-isopentenyl)-adenosine (MS[2]I[6]A)
CC       adjacent to the anticodon of several tRNA species.
CC   -!- CATALYTIC ACTIVITY: Isopentenyl diphosphate + tRNA = diphosphate +
CC       tRNA containing 6-isopentenyladenosine.
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DR   EMBL; AP009152; BAG29928.1; -; Genomic_DNA.
DR   RefSeq; YP_001855434.1; -.
DR   GeneID; 6239346; -.
DR   GenomeReviews; AP009152_GR; KRH_15810.
DR   KEGG; krh:KRH_15810; -.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0004811; F:tRNA isopentenyltransferase activity; IEA:InterPro.
DR   GO; GO:0008033; P:tRNA processing; IEA:InterPro.
DR   InterPro; IPR002627; IPPT.
DR   PANTHER; PTHR11088; IPPT; 1.
DR   Pfam; PF01715; IPPT; 1.
DR   TIGRFAMs; TIGR00174; miaA; 1.
PE   4: Predicted;
KW   ATP-binding; Complete proteome; Nucleotide-binding;
KW   Nucleotidyltransferase; Transferase; tRNA processing.
SQ   SEQUENCE   313 AA;  34331 MW;  F39C134E5CE81678 CRC64;
     MSAPDAGRED TRIPLVAVVG PTGTGKSELA IALARELDGE VVNADALQLY RGMDVGTAKL
     TPEERQGVPH HLLDVLEIHE EASVAAFQRD ARRAVDEIRG RGRVPVLVGG SGLYVRAALD
     AIEFPGTDAT VRARREEQLR ERGRAALLRE LAAVDPGSAE RVKDDRRLVR ALEVHDLTGR
     PFTSFMPERR YVQPTVQIGL AMDREVLNRR LAHRVDLMLE RGWLEEVKAL EARGLRESPT
     AGRALGYPQL LAVLDGSATL DEAREDTVAA TRRFTKRQRT WFGADPRVHW LDAGDPNGID
     GLTAGAVRLV AAQ
//