ID   B1VCC4_ECOLX            Unreviewed;       406 AA.
AC   B1VCC4;
DT   20-MAY-2008, integrated into UniProtKB/TrEMBL.
DT   20-MAY-2008, sequence version 1.
DT   22-NOV-2017, entry version 70.
DE   RecName: Full=Arginine deiminase {ECO:0000256|HAMAP-Rule:MF_00242};
DE            Short=ADI {ECO:0000256|HAMAP-Rule:MF_00242};
DE            EC=3.5.3.6 {ECO:0000256|HAMAP-Rule:MF_00242};
DE   AltName: Full=Arginine dihydrolase {ECO:0000256|HAMAP-Rule:MF_00242};
DE            Short=AD {ECO:0000256|HAMAP-Rule:MF_00242};
GN   Name=ArcA {ECO:0000313|EMBL:CAP07746.1};
GN   Synonyms=arcA {ECO:0000256|HAMAP-Rule:MF_00242,
GN   ECO:0000313|EMBL:AKJ21526.1};
GN   ORFNames=AM270_23690 {ECO:0000313|EMBL:KQJ37053.1}, BE932_01655
GN   {ECO:0000313|EMBL:AQV71254.1}, BE957_01645
GN   {ECO:0000313|EMBL:AQV17959.1}, BHS81_29890
GN   {ECO:0000313|EMBL:ORJ63243.1}, BZL31_24880
GN   {ECO:0000313|EMBL:OUK47148.1}, CI718_01760
GN   {ECO:0000313|EMBL:OYK47312.1}, ETN48_p0050
GN   {ECO:0000313|EMBL:CBX35976.1}, IPF_255 {ECO:0000313|EMBL:CAP07746.1},
GN   pCTXM15_EC8_00035 {ECO:0000313|EMBL:AKJ21526.1};
OS   Escherichia coli.
OG   Plasmid pCA14 {ECO:0000313|EMBL:AKD26516.1},
OG   Plasmid pCA28 {ECO:0000313|EMBL:AKD26643.1},
OG   Plasmid pCTXM15 {ECO:0000313|EMBL:AKJ21526.1},
OG   Plasmid pEC224_1 {ECO:0000313|EMBL:AQV17959.1},
OG   Plasmid pECAZ146_1 {ECO:0000313|EMBL:AQV71254.1},
OG   Plasmid pETN48 {ECO:0000313|EMBL:CBX35976.1},
OG   Plasmid pIP1206 {ECO:0000313|EMBL:CAP07746.1},
OG   Plasmid pec224_1 {ECO:0000313|Proteomes:UP000189671}, and
OG   Plasmid pecaz146_1 {ECO:0000313|Proteomes:UP000189626}.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562 {ECO:0000313|EMBL:CAP07746.1};
RN   [1] {ECO:0000313|EMBL:CAP07746.1}
RP   NUCLEOTIDE SEQUENCE.
RC   PLASMID=pIP1206 {ECO:0000313|EMBL:CAP07746.1};
RA   Perichon B., Bogaerts P., Lambert T., Frangeul L., Courvalin P.,
RA   Galimand M.;
RT   "Sequence of conjugative plasmid pIP1206 mediating resistance to
RT   aminoglycosides by 16S rRNA methylation and to hydrophilic
RT   fluoroquinolones.";
RL   Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:CBX35976.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=O102-ST405 {ECO:0000313|EMBL:CBX35976.1};
RC   PLASMID=pETN48 {ECO:0000313|EMBL:CBX35976.1};
RA   Billard-Pomares T., Tenaillon O., Le Nagard H., Rouy Z.,
RA   Cruvellier S., Medigue C., Arlet G., Denamur E., Branger C.;
RT   "Complete nucleotide sequence of plasmid pTN48 encoding the CTX-M-14
RT   extended spectrum beta-lactamase from an Escherichia coli O102-ST405
RT   strain.";
RL   Submitted (OCT-2010) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0000313|EMBL:CBX35976.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=O102-ST405 {ECO:0000313|EMBL:CBX35976.1};
RC   PLASMID=pETN48 {ECO:0000313|EMBL:CBX35976.1};
RA   Genoscope - CEA;
RL   Submitted (OCT-2010) to the EMBL/GenBank/DDBJ databases.
RN   [4] {ECO:0000313|EMBL:AKD26643.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=CA28 {ECO:0000313|EMBL:AKD26643.1};
RC   PLASMID=pCA28 {ECO:0000313|EMBL:AKD26643.1};
RA   Li J., Doi Y.;
RT   "CTX-M-encoding plasmid pCA28 from community associated ESBL-producing
RT   E.coli.";
RL   Submitted (AUG-2014) to the EMBL/GenBank/DDBJ databases.
RN   [5] {ECO:0000313|EMBL:AKD26516.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=CA14 {ECO:0000313|EMBL:AKD26516.1}, and CA28
RC   {ECO:0000313|EMBL:AKD26643.1};
RC   PLASMID=pCA14 {ECO:0000313|EMBL:AKD26516.1}, and pCA28
RC   {ECO:0000313|EMBL:AKD26643.1};
RX   PubMed=25753630; DOI=10.1128/AAC.04772-14;
RA   Li J.J., Spychala C.N., Hu F., Sheng J.F., Doi Y.;
RT   "Complete nucleotide sequences of bla(CTX-M)-harboring IncF plasmids
RT   from community-associated Escherichia coli strains in the United
RT   States.";
RL   Antimicrob. Agents Chemother. 59:3002-3007(2015).
RN   [6] {ECO:0000313|EMBL:AKJ21526.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=WCHEC13-8 {ECO:0000313|EMBL:AKJ21526.1};
RC   PLASMID=pCTXM15 {ECO:0000313|EMBL:AKJ21526.1};
RA   Zong Z.;
RL   Submitted (FEB-2015) to the EMBL/GenBank/DDBJ databases.
RN   [7] {ECO:0000313|EMBL:AKD26516.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=CA14 {ECO:0000313|EMBL:AKD26516.1};
RC   PLASMID=pCA14 {ECO:0000313|EMBL:AKD26516.1};
RA   Li J.-J., Doi Y.;
RT   "CTX-M-encoding plasmid pCA14 from community associated ESBL-producing
RT   E.coli.";
RL   Submitted (APR-2015) to the EMBL/GenBank/DDBJ databases.
RN   [8] {ECO:0000313|EMBL:KQJ37053.1, ECO:0000313|Proteomes:UP000051846}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=304 {ECO:0000313|EMBL:KQJ37053.1,
RC   ECO:0000313|Proteomes:UP000051846};
RA   Kudirkiene E., Olsen J.E.;
RT   "Characterization of Escherichia coli strains isolated from cattle and
RT   human in Tanzania.";
RL   Submitted (AUG-2015) to the EMBL/GenBank/DDBJ databases.
RN   [9] {ECO:0000313|EMBL:AQV17959.1, ECO:0000313|Proteomes:UP000189626, ECO:0000313|Proteomes:UP000189671}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ecol_224 {ECO:0000313|EMBL:AQV17959.1,
RC   ECO:0000313|Proteomes:UP000189671}, and Ecol_AZ146
RC   {ECO:0000313|EMBL:AQV71254.1, ECO:0000313|Proteomes:UP000189626};
RC   PLASMID=pEC224_1 {ECO:0000313|EMBL:AQV17959.1}, pECAZ146_1
RC   {ECO:0000313|EMBL:AQV71254.1}, Plasmid pec224_1
RC   {ECO:0000313|Proteomes:UP000189671}, and Plasmid pecaz146_1
RC   {ECO:0000313|Proteomes:UP000189626};
RA   Song W.-J., Kurnit D.M.;
RL   Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
RN   [10] {ECO:0000313|EMBL:OUK47148.1, ECO:0000313|Proteomes:UP000194908}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SJRP/Ec42664 {ECO:0000313|EMBL:OUK47148.1};
RA   Casella T., Cerdeira L., Lincopan N., Nogueira M.C.;
RT   "Draft genome sequence of Escherichia coli strain SJRP/Ec42664.";
RL   Submitted (JAN-2017) to the EMBL/GenBank/DDBJ databases.
RN   [11] {ECO:0000313|EMBL:ORJ63243.1, ECO:0000313|Proteomes:UP000193168}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KCJK1866 {ECO:0000313|EMBL:ORJ63243.1,
RC   ECO:0000313|Proteomes:UP000193168};
RA   Afonso C.L., Miller P.J., Scott M.A., Spackman E., Goraichik I.,
RA   Dimitrov K.M., Suarez D.L., Swayne D.E.;
RL   Submitted (MAR-2017) to the EMBL/GenBank/DDBJ databases.
RN   [12] {ECO:0000313|EMBL:OYK47312.1, ECO:0000313|Proteomes:UP000215519}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ECPUTH02 {ECO:0000313|EMBL:OYK47312.1,
RC   ECO:0000313|Proteomes:UP000215519};
RA   de Groot N.N.;
RL   Submitted (AUG-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: L-arginine + H(2)O = L-citrulline + NH(3).
CC       {ECO:0000256|HAMAP-Rule:MF_00242, ECO:0000256|SAAS:SAAS00945390}.
CC   -!- PATHWAY: Amino-acid degradation; L-arginine degradation via ADI
CC       pathway; carbamoyl phosphate from L-arginine: step 1/2.
CC       {ECO:0000256|HAMAP-Rule:MF_00242, ECO:0000256|SAAS:SAAS00945388}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00242,
CC       ECO:0000256|SAAS:SAAS00945386}.
CC   -!- SIMILARITY: Belongs to the arginine deiminase family.
CC       {ECO:0000256|HAMAP-Rule:MF_00242, ECO:0000256|SAAS:SAAS00945383}.
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DR   EMBL; CP009231; AKD26516.1; -; Genomic_DNA.
DR   EMBL; CP009232; AKD26643.1; -; Genomic_DNA.
DR   EMBL; KP789020; AKJ21526.1; -; Genomic_DNA.
DR   EMBL; CP018947; AQV17959.1; -; Genomic_DNA.
DR   EMBL; CP018990; AQV71254.1; -; Genomic_DNA.
DR   EMBL; AM886293; CAP07746.1; -; Genomic_DNA.
DR   EMBL; FQ482074; CBX35976.1; -; Genomic_DNA.
DR   EMBL; LIVI01000045; KQJ37053.1; -; Genomic_DNA.
DR   EMBL; NAHY01000791; ORJ63243.1; -; Genomic_DNA.
DR   EMBL; MUAB01000077; OUK47148.1; -; Genomic_DNA.
DR   EMBL; NQDM01000011; OYK47312.1; -; Genomic_DNA.
DR   RefSeq; WP_000410951.1; NZ_NQDM01000011.1.
DR   RefSeq; YP_001816560.1; NC_010558.1.
DR   RefSeq; YP_003937657.1; NC_014615.1.
DR   EnsemblBacteria; KLX81364; KLX81364; SK83_04739.
DR   EnsemblBacteria; KQJ37053; KQJ37053; AM270_23690.
DR   EnsemblBacteria; KUU48094; KUU48094; AWF22_25035.
DR   EnsemblBacteria; ODH26479; ODH26479; A6803_24370.
DR   GeneID; 6276097; -.
DR   GeneID; 9845996; -.
DR   PATRIC; fig|562.10494.peg.1666; -.
DR   UniPathway; UPA00254; UER00364.
DR   Proteomes; UP000051846; Unassembled WGS sequence.
DR   Proteomes; UP000189626; Plasmid pecaz146_1.
DR   Proteomes; UP000189671; Plasmid pec224_1.
DR   Proteomes; UP000193168; Unassembled WGS sequence.
DR   Proteomes; UP000194908; Unassembled WGS sequence.
DR   Proteomes; UP000215519; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016990; F:arginine deiminase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019547; P:arginine catabolic process to ornithine; IEA:UniProtKB-UniPathway.
DR   HAMAP; MF_00242; Arg_deiminase; 1.
DR   InterPro; IPR003876; Arg_deiminase.
DR   InterPro; IPR033199; DDAH/AD.
DR   PANTHER; PTHR12737; PTHR12737; 1.
DR   PANTHER; PTHR12737:SF22; PTHR12737:SF22; 1.
DR   PIRSF; PIRSF006356; Arg_deiminase; 1.
DR   PRINTS; PR01466; ARGDEIMINASE.
DR   TIGRFAMs; TIGR01078; arcA; 1.
PE   3: Inferred from homology;
KW   Arginine metabolism {ECO:0000256|HAMAP-Rule:MF_00242,
KW   ECO:0000256|SAAS:SAAS00945389};
KW   Complete proteome {ECO:0000313|Proteomes:UP000051846,
KW   ECO:0000313|Proteomes:UP000189626, ECO:0000313|Proteomes:UP000189671,
KW   ECO:0000313|Proteomes:UP000193168};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00242,
KW   ECO:0000256|SAAS:SAAS00945391};
KW   Hydrolase {ECO:0000256|HAMAP-Rule:MF_00242,
KW   ECO:0000256|SAAS:SAAS00945385, ECO:0000313|EMBL:AKD26516.1};
KW   Plasmid {ECO:0000313|EMBL:CAP07746.1}.
FT   ACT_SITE    396    396       Amidino-cysteine intermediate.
FT                                {ECO:0000256|HAMAP-Rule:MF_00242,
FT                                ECO:0000256|PIRSR:PIRSR006356-1}.
SQ   SEQUENCE   406 AA;  45773 MW;  351D3EAF743554BD CRC64;
     MEKHYVGSEI GQLRSVMLHR PNLSLKRLTP SNCQELLFDD VLSVERAGEE HDIFANTLRD
     QGVEVLLLTD LLTQTLDIKE AKTWLLETQI SDYRLGPTFA GDVRSWLADM PHRELARRLS
     GGLTYGEIPA AINNMVVDTH TSNDFIMKPL PNHLFTRDTS CWIYNGVSIN PMAKPARQRE
     TNNLRAIYRW HPAFADGDFI KYFGDENIYY DHATLEGGDV LVIGRGAVLI GMSERTTPQG
     VEFLANSLFK HRQAERVIAV ELPKHRSCMH LDTVMTHIDV DTFSVYPEVV RKDAQCWTLT
     SNGRDGLQRT QETDLLHAIE KALGIDQVRL ITTGGDAFEA EREQWNDANN VLTIRPGVVI
     GYERNVWTNE KYDKAGITVL PIPGDELGRG RGGARCMSCP LERDGI
//