ID CDK20_DANRE Reviewed; 344 AA. AC A8WIP6; Q5PRE2; DT 20-APR-2010, integrated into UniProtKB/Swiss-Prot. DT 15-JAN-2008, sequence version 1. DT 08-FEB-2011, entry version 31. DE RecName: Full=Cyclin-dependent kinase 20; DE EC=2.7.11.22; DE AltName: Full=Cell cycle-related kinase; DE AltName: Full=Cell division protein kinase 20; GN Name=cdk20; Synonyms=ccrk; ORFNames=si:dkeyp-77f7.1, zgc:101530; OS Danio rerio (Zebrafish) (Brachydanio rerio). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; OC Cyprinidae; Danio. OX NCBI_TaxID=7955; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Tuebingen; RG The Danio rerio sequencing project at the Sanger Institute; RL Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases. RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Brain; RG NIH - Zebrafish Gene Collection (ZGC) project; RL Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases. RN [3] RP DISRUPTION PHENOTYPE, AND FUNCTION. RX PubMed=20159594; DOI=10.1016/j.devcel.2009.12.014; RA Ko H.W., Norman R.X., Tran J., Fuller K.P., Fukuda M., RA Eggenschwiler J.T.; RT "Broad-minded links cell cycle-related kinase to cilia assembly and RT hedgehog signal transduction."; RL Dev. Cell 18:237-247(2010). CC -!- FUNCTION: Involved in cell growth. Activates cdk2, a kinase CC involved in the control of the cell cycle, by phosphorylating CC residue 'Thr-160' (By similarity). Required for high-level Shh CC responses in the developing neural tube. Together with bromi, CC controls the structure of the primary cilium by coordinating CC assembly of the ciliary membrane and axoneme, allowing gli2 to be CC properly activated in response to SHH signaling. CC -!- CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. CC -!- SUBUNIT: Monomer (By similarity). Interacts with BROMI (By CC similarity). CC -!- SUBCELLULAR LOCATION: Nucleus (By similarity). Cytoplasm (By CC similarity). Cell projection, cilium (By similarity). CC -!- DISRUPTION PHENOTYPE: Fish lacking cdk20 are viable during CC embryogenesis and early larval stages, but exhibit curvature of CC the body axis and curled cilia in distal kidney tubules. CC -!- SIMILARITY: Belongs to the protein kinase superfamily. CMGC CC Ser/Thr protein kinase family. CDC2/CDKX subfamily. CC -!- SIMILARITY: Contains 1 protein kinase domain. CC -!- SEQUENCE CAUTION: CC Sequence=AAH86697.1; Type=Frameshift; Positions=84; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; BX571687; CAP19557.1; -; Genomic_DNA. DR EMBL; BC086697; AAH86697.1; ALT_FRAME; mRNA. DR IPI; IPI00484737; -. DR RefSeq; NP_001008655.1; NM_001008655.1. DR UniGene; Dr.37814; -. DR ProteinModelPortal; A8WIP6; -. DR SMR; A8WIP6; 1-288. DR Ensembl; ENSDART00000021607; ENSDARP00000006661; ENSDARG00000003867. DR GeneID; 494112; -. DR KEGG; dre:494112; -. DR CTD; 494112; -. DR ZFIN; ZDB-GENE-041212-84; zgc:101530. DR eggNOG; fiNOG07196; -. DR HOGENOM; HBG755340; -. DR InParanoid; Q5PRE2; -. DR OrthoDB; EOG90CM34; -. DR PhylomeDB; Q5PRE2; -. DR Bgee; A8WIP6; -. DR GO; GO:0005929; C:cilium; IEA:UniProtKB-SubCell. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0004693; F:cyclin-dependent protein kinase activity; IEA:EC. DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW. DR GO; GO:0060271; P:cilium morphogenesis; IGI:ZFIN. DR GO; GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW. DR GO; GO:0006468; P:protein phosphorylation; IEA:InterPro. DR InterPro; IPR011009; Kinase-like_dom. DR InterPro; IPR000719; Prot_kinase_cat_dom. DR InterPro; IPR017441; Protein_kinase_ATP_BS. DR InterPro; IPR017442; Se/Thr_prot_kinase-like_dom. DR InterPro; IPR008271; Ser/Thr_prot_kinase_AS. DR InterPro; IPR002290; Ser/Thr_prot_kinase_dom. DR Pfam; PF00069; Pkinase; 1. DR SMART; SM00220; S_TKc; 1. DR SUPFAM; SSF56112; Kinase_like; 1. DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1. DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1. DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1. PE 2: Evidence at transcript level; KW ATP-binding; Cell cycle; Cell division; Cell projection; Cilium; KW Cytoplasm; Developmental protein; Kinase; Nucleotide-binding; Nucleus; KW Serine/threonine-protein kinase; Transferase. FT CHAIN 1 344 Cyclin-dependent kinase 20. FT /FTId=PRO_0000393616. FT DOMAIN 4 288 Protein kinase. FT NP_BIND 10 18 ATP (By similarity). FT ACT_SITE 127 127 Proton acceptor (By similarity). FT BINDING 33 33 ATP (By similarity). SQ SEQUENCE 344 AA; 39025 MW; D4C820FAF9C7FB62 CRC64; MDQYSILGRI GEGAHGIVFK AKHIETGETV ALKKVALRRL EDGIPNQALR EIKALQEIED NQYVVKLKDV FPHGTGFVLV FEYMLSDLSE VIRNSQRPLT ASQVKSYMMM LLKGVAFCHE NSIMHRDLKP ANLLISSTGH LKIADFGLAR LFSNEGDRLY SHQVATRWYR APELLYGARK YDEGVDLWAV GCIFGELLNN SPLFPGENDI EQLCCVLRVL GTPNQKVWPE ITELPDYNKI TFKENPPIPL EEIVPDTSPQ AVDLLKKFLV YPSKQRISAR QALLHPYFFT DPLPAHHSEL PIPQRGGKHS RQRMQPPHEF TVDRPLHESV VDPSLIQKHA MSCS //