ID CDK20_DANRE Reviewed; 344 AA. AC A8WIP6; Q5PRE2; DT 20-APR-2010, integrated into UniProtKB/Swiss-Prot. DT 15-JAN-2008, sequence version 1. DT 24-JAN-2024, entry version 104. DE RecName: Full=Cyclin-dependent kinase 20; DE EC=2.7.11.22; DE AltName: Full=Cell cycle-related kinase; DE AltName: Full=Cell division protein kinase 20; GN Name=cdk20; Synonyms=ccrk; ORFNames=si:dkeyp-77f7.1, zgc:101530; OS Danio rerio (Zebrafish) (Brachydanio rerio). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; OC Danionidae; Danioninae; Danio. OX NCBI_TaxID=7955; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Tuebingen; RX PubMed=23594743; DOI=10.1038/nature12111; RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J., RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G., RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P., RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.; RT "The zebrafish reference genome sequence and its relationship to the human RT genome."; RL Nature 496:498-503(2013). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Brain; RG NIH - Zebrafish Gene Collection (ZGC) project; RL Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases. RN [3] RP DISRUPTION PHENOTYPE, AND FUNCTION. RX PubMed=20159594; DOI=10.1016/j.devcel.2009.12.014; RA Ko H.W., Norman R.X., Tran J., Fuller K.P., Fukuda M., Eggenschwiler J.T.; RT "Broad-minded links cell cycle-related kinase to cilia assembly and RT hedgehog signal transduction."; RL Dev. Cell 18:237-247(2010). CC -!- FUNCTION: Involved in cell growth. Activates cdk2, a kinase involved in CC the control of the cell cycle, by phosphorylating residue 'Thr-160' (By CC similarity). Required for high-level Shh responses in the developing CC neural tube. Together with tbc1d32, controls the structure of the CC primary cilium by coordinating assembly of the ciliary membrane and CC axoneme, allowing gli2 to be properly activated in response to SHH CC signaling. {ECO:0000250, ECO:0000269|PubMed:20159594}. CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl- CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA- CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.22; CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L- CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; CC EC=2.7.11.22; CC -!- SUBUNIT: Monomer. Interacts with tbc1d32. {ECO:0000250}. CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}. CC Cell projection, cilium {ECO:0000250}. CC -!- DISRUPTION PHENOTYPE: Fish lacking cdk20 are viable during CC embryogenesis and early larval stages, but exhibit curvature of the CC body axis and curled cilia in distal kidney tubules. CC {ECO:0000269|PubMed:20159594}. CC -!- SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr CC protein kinase family. CDC2/CDKX subfamily. {ECO:0000305}. CC -!- SEQUENCE CAUTION: CC Sequence=AAH86697.1; Type=Frameshift; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; BX571687; CAP19557.1; -; Genomic_DNA. DR EMBL; BC086697; AAH86697.1; ALT_FRAME; mRNA. DR RefSeq; NP_001008655.1; NM_001008655.1. DR RefSeq; XP_005166746.1; XM_005166689.3. DR AlphaFoldDB; A8WIP6; -. DR SMR; A8WIP6; -. DR STRING; 7955.ENSDARP00000113146; -. DR PaxDb; 7955-ENSDARP00000113146; -. DR GeneID; 494112; -. DR KEGG; dre:494112; -. DR CTD; 23552; -. DR ZFIN; ZDB-GENE-041212-84; cdk20. DR eggNOG; KOG0659; Eukaryota. DR HOGENOM; CLU_000288_181_1_1; -. DR InParanoid; A8WIP6; -. DR OMA; KITFPYH; -. DR OrthoDB; 244018at2759; -. DR PhylomeDB; A8WIP6; -. DR TreeFam; TF327240; -. DR PRO; PR:A8WIP6; -. DR Proteomes; UP000000437; Chromosome 8. DR Bgee; ENSDARG00000003867; Expressed in testis and 15 other cell types or tissues. DR ExpressionAtlas; A8WIP6; baseline. DR GO; GO:0005929; C:cilium; IEA:UniProtKB-SubCell. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0005634; C:nucleus; IBA:GO_Central. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0004693; F:cyclin-dependent protein serine/threonine kinase activity; IEA:UniProtKB-EC. DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA. DR GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central. DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW. DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW. DR GO; GO:0060271; P:cilium assembly; IMP:ZFIN. DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW. DR CDD; cd07832; STKc_CCRK; 1. DR Gene3D; 1.10.510.10; Transferase(Phosphotransferase) domain 1; 1. DR InterPro; IPR048002; CDK20-like_STKc. DR InterPro; IPR011009; Kinase-like_dom_sf. DR InterPro; IPR000719; Prot_kinase_dom. DR InterPro; IPR017441; Protein_kinase_ATP_BS. DR InterPro; IPR008271; Ser/Thr_kinase_AS. DR PANTHER; PTHR24056; CELL DIVISION PROTEIN KINASE; 1. DR PANTHER; PTHR24056:SF171; CYCLIN-DEPENDENT KINASE 20; 1. DR Pfam; PF00069; Pkinase; 1. DR SMART; SM00220; S_TKc; 1. DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1. DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1. DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1. DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1. PE 2: Evidence at transcript level; KW ATP-binding; Cell cycle; Cell division; Cell projection; Cilium; Cytoplasm; KW Developmental protein; Kinase; Nucleotide-binding; Nucleus; KW Reference proteome; Serine/threonine-protein kinase; Transferase. FT CHAIN 1..344 FT /note="Cyclin-dependent kinase 20" FT /id="PRO_0000393616" FT DOMAIN 4..288 FT /note="Protein kinase" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159" FT ACT_SITE 127 FT /note="Proton acceptor" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159, FT ECO:0000255|PROSITE-ProRule:PRU10027" FT BINDING 10..18 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159" FT BINDING 33 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159" SQ SEQUENCE 344 AA; 39025 MW; D4C820FAF9C7FB62 CRC64; MDQYSILGRI GEGAHGIVFK AKHIETGETV ALKKVALRRL EDGIPNQALR EIKALQEIED NQYVVKLKDV FPHGTGFVLV FEYMLSDLSE VIRNSQRPLT ASQVKSYMMM LLKGVAFCHE NSIMHRDLKP ANLLISSTGH LKIADFGLAR LFSNEGDRLY SHQVATRWYR APELLYGARK YDEGVDLWAV GCIFGELLNN SPLFPGENDI EQLCCVLRVL GTPNQKVWPE ITELPDYNKI TFKENPPIPL EEIVPDTSPQ AVDLLKKFLV YPSKQRISAR QALLHPYFFT DPLPAHHSEL PIPQRGGKHS RQRMQPPHEF TVDRPLHESV VDPSLIQKHA MSCS //