ID CCBE1_DANRE Reviewed; 401 AA. AC A8WGB1; DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot. DT 05-MAY-2009, sequence version 2. DT 16-JUN-2009, entry version 13. DE RecName: Full=Collagen and calcium-binding EGF domain-containing protein 1; DE AltName: Full=Full of fluid protein; DE Flags: Precursor; GN Name=ccbe1; Synonyms=fof; OS Danio rerio (Zebrafish) (Brachydanio rerio). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; OC Cyprinidae; Danio. OX NCBI_TaxID=7955; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RG NIH - Zebrafish Gene Collection (ZGC) project; RL Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases. RN [2] RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF RP ASP-162. RX PubMed=19287381; DOI=10.1038/ng.321; RA Hogan B.M., Bos F.L., Bussmann J., Witte M., Chi N.C., Duckers H.J., RA Schulte-Merker S.; RT "ccbe1 is required for embryonic lymphangiogenesis and venous RT sprouting."; RL Nat. Genet. 41:396-398(2009). CC -!- FUNCTION: Required for lymphangioblast budding and angiogenic CC sprouting from venous endothelium during embryogenesis. Necessary CC for lymphangiogenesis, but is probably not part of either the CC vegfc-vegfr3 signaling or sox18-prox1 transcriptional pathways. CC -!- SUBCELLULAR LOCATION: Secreted (Potential). CC -!- TISSUE SPECIFICITY: Not expressed in blood or lymphatic CC endothelial cells, correlating spatially and temporally with the CC migration routes of endothelial cells that bud from the PCV, CC migrate in association with somite boundaries and seed the CC horizontal myoseptum region from where lymphatic precursors later CC migrate. CC -!- DEVELOPMENTAL STAGE: Restricted expression during development with CC expression in the pronephros and ventral mesenchyme at 24 hours CC post fertilization (hpf). By 36 hpf, expression is detected in CC discrete zones along each somitic boundary, between the PCV and CC the horizontal myoseptum, as well as along the horizontal CC myoseptum itself. At 48 hpf, expression is restricted along the CC horizontal myoseptum. CC -!- SIMILARITY: Belongs to the CCBE1 family. CC -!- SIMILARITY: Contains 2 collagen-like domains. CC -!- SIMILARITY: Contains 1 EGF-like domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; BC154643; AAI54644.1; ALT_INIT; mRNA. DR IPI; IPI00896862; -. DR RefSeq; XP_001923108.1; -. DR UniGene; Dr.110996; -. DR Ensembl; ENSDARG00000060022; Danio rerio. DR GeneID; 555629; -. DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell. DR GO; GO:0005509; F:calcium ion binding; IEA:UniProtKB-KW. DR GO; GO:0001946; P:lymphangiogenesis; IMP:UniProtKB. DR GO; GO:0002040; P:sprouting angiogenesis; IMP:UniProtKB. DR GO; GO:0048845; P:venous blood vessel morphogenesis; IMP:UniProtKB. DR InterPro; IPR008160; Collagen. DR InterPro; IPR006210; EGF-like. DR InterPro; IPR013032; EGF-like_reg_CS. DR InterPro; IPR000152; EGF-type_Asp/Asn_hydroxyl_CS. DR InterPro; IPR000742; EGF_3. DR InterPro; IPR001881; EGF_Ca_bd. DR InterPro; IPR013091; EGF_Ca_bd_2. DR InterPro; IPR018097; EGF_Ca_bd_CS. DR Pfam; PF01391; Collagen; 2. DR Pfam; PF07645; EGF_CA; 1. DR SMART; SM00181; EGF; 1. DR SMART; SM00179; EGF_CA; 1. DR PROSITE; PS00010; ASX_HYDROXYL; 1. DR PROSITE; PS00022; EGF_1; FALSE_NEG. DR PROSITE; PS01186; EGF_2; 1. DR PROSITE; PS50026; EGF_3; 1. DR PROSITE; PS01187; EGF_CA; 1. PE 1: Evidence at protein level; KW Angiogenesis; Calcium; Collagen; Developmental protein; KW Disulfide bond; EGF-like domain; Glycoprotein; Repeat; Secreted; KW Signal. FT SIGNAL 1 22 Potential. FT CHAIN 23 401 Collagen and calcium-binding EGF domain- FT containing protein 1. FT /FTId=PRO_0000370636. FT DOMAIN 126 167 EGF-like; calcium-binding (Potential). FT DOMAIN 234 276 Collagen-like 1. FT DOMAIN 286 319 Collagen-like 2. FT COMPBIAS 236 319 Gly-rich. FT CARBOHYD 134 134 N-linked (GlcNAc...) (Potential). FT DISULFID 130 142 By similarity. FT DISULFID 138 151 By similarity. FT DISULFID 153 166 By similarity. FT MUTAGEN 162 162 D->E: In fof(hu3613); lacks the thoracic FT duct and the previously unidentified FT intersegmental (ISLVs) and dorsal FT longitudinal lymphatic vessels (DLLVs) FT but retains a seemingly normal blood FT vasculature. Mutants show edema, FT initiating in the lower intestine and FT around the eyes from 6 dpf. At 36 dpf, FT surviving mutants show severe edema. SQ SEQUENCE 401 AA; 43849 MW; 27F2BB0FF261DCBC CRC64; MIYPGRGASL SVAVALVLFS SGAPWTFREE KEDVDREVCS ESKIATTKYP CVKSTGEVTT CYRKKCCEGF KFVLGQCIPE DYDVCAGAPC EQQCTDHFGR VVCTCYDGYR YDRERHRNRE KPYCLDIDEC ANNNETVCSQ MCVNTPGSYR CDCHSGFYLE DDGKTCTKGE RAPLFEKSDN VMKEGTCSAT CEDFHQMKMT VLQLKQKMSL LSSNTEINKQ MTNEKMMMTT NSFLPGPPGP PGPAGTPGAK GSSGSPGQMG PPGLPGPRGD MGPIGPSPDL SHIKQGRRGP VGPPGAPGRD GMKGERGFPG PSGPPGPPGS FDFLLLMMAD IRNDIAELQS KVFSRPLHSS FEDFPSAPDS WRDTPENLDF GSGEDYKSQS PPKSSRKRKL PRNLKNPDWP V //