ID A8BFP4_GIAIC Unreviewed; 114 AA. AC A8BFP4; DT 13-NOV-2007, integrated into UniProtKB/TrEMBL. DT 13-NOV-2007, sequence version 1. DT 24-JUL-2024, entry version 75. DE RecName: Full=L-dopachrome isomerase {ECO:0000256|ARBA:ARBA00042730}; DE EC=5.3.2.1 {ECO:0000256|ARBA:ARBA00039086}; DE EC=5.3.3.12 {ECO:0000256|ARBA:ARBA00038932}; DE AltName: Full=L-dopachrome tautomerase {ECO:0000256|ARBA:ARBA00041631}; DE AltName: Full=Phenylpyruvate tautomerase {ECO:0000256|ARBA:ARBA00041912}; GN ORFNames=GL50803_0012091 {ECO:0000313|EMBL:KAE8303400.1}, GN GL50803_12091 {ECO:0000313|EMBL:EDO79637.1}; OS Giardia intestinalis (strain ATCC 50803 / WB clone C6) (Giardia lamblia). OC Eukaryota; Metamonada; Diplomonadida; Hexamitidae; Giardiinae; Giardia. OX NCBI_TaxID=184922 {ECO:0000313|EMBL:EDO79637.1}; RN [1] {ECO:0000313|EMBL:EDO79637.1, ECO:0000313|Proteomes:UP000001548} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 50803 / WB clone C6 {ECO:0000313|Proteomes:UP000001548}, RC and WB C6 {ECO:0000313|EMBL:EDO79637.1}; RX PubMed=17901334; DOI=10.1126/science.1143837; RA Morrison H.G., McArthur A.G., Gillin F.D., Aley S.B., Adam R.D., RA Olsen G.J., Best A.A., Cande W.Z., Chen F., Cipriano M.J., Davids B.J., RA Dawson S.C., Elmendorf H.G., Hehl A.B., Holder M.E., Huse S.M., Kim U.U., RA Lasek-Nesselquist E., Manning G., Nigam A., Nixon J.E., Palm D., RA Passamaneck N.E., Prabhu A., Reich C.I., Reiner D.S., Samuelson J., RA Svard S.G., Sogin M.L.; RT "Genomic minimalism in the early diverging intestinal parasite Giardia RT lamblia."; RL Science 317:1921-1926(2007). RN [2] {ECO:0007829|PDB:3T5S} RP X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS). RX PubMed=23709284; DOI=10.1007/s10969-013-9155-9; RA Buchko G.W., Abendroth J., Robinson H., Zhang Y., Hewitt S.N., RA Edwards T.E., Van Voorhis W.C., Myler P.J.; RT "Crystal structure of a macrophage migration inhibitory factor from Giardia RT lamblia."; RL J. Struct. Funct. Genomics 14:47-57(2013). RN [3] {ECO:0000313|EMBL:KAE8303400.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=WB C6 {ECO:0000313|EMBL:KAE8303400.1}; RA Xu F., Jex A., Svard S.G.; RT "New Giardia intestinalis WB genome in near-complete chromosomes."; RL Submitted (JUL-2019) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=3-phenylpyruvate = enol-phenylpyruvate; Xref=Rhea:RHEA:17097, CC ChEBI:CHEBI:16815, ChEBI:CHEBI:18005; EC=5.3.2.1; CC Evidence={ECO:0000256|ARBA:ARBA00036735}; CC -!- CATALYTIC ACTIVITY: CC Reaction=L-dopachrome = 5,6-dihydroxyindole-2-carboxylate; CC Xref=Rhea:RHEA:13041, ChEBI:CHEBI:16875, ChEBI:CHEBI:57509; CC EC=5.3.3.12; Evidence={ECO:0000256|ARBA:ARBA00036823}; CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}. CC -!- SIMILARITY: Belongs to the MIF family. {ECO:0000256|ARBA:ARBA00005851}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:EDO79637.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AACB02000015; EDO79637.1; -; Genomic_DNA. DR EMBL; AACB03000002; KAE8303400.1; -; Genomic_DNA. DR RefSeq; XP_001707311.1; XM_001707259.1. DR PDB; 3T5S; X-ray; 2.30 A; A=1-114. DR PDBsum; 3T5S; -. DR AlphaFoldDB; A8BFP4; -. DR SMR; A8BFP4; -. DR STRING; 184922.A8BFP4; -. DR EnsemblProtists; EDO79637; EDO79637; GL50803_12091. DR GeneID; 5700210; -. DR KEGG; gla:GL50803_0012091; -. DR VEuPathDB; GiardiaDB:GL50803_12091; -. DR HOGENOM; CLU_129906_4_1_1; -. DR InParanoid; A8BFP4; -. DR OMA; CELITNI; -. DR EvolutionaryTrace; A8BFP4; -. DR Proteomes; UP000001548; Chromosome 4. DR GO; GO:0005615; C:extracellular space; IBA:GO_Central. DR GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW. DR GO; GO:0050178; F:phenylpyruvate tautomerase activity; IBA:GO_Central. DR Gene3D; 3.30.429.10; Macrophage Migration Inhibitory Factor; 1. DR InterPro; IPR001398; Macrophage_inhib_fac. DR InterPro; IPR014347; Tautomerase/MIF_sf. DR PANTHER; PTHR11954; D-DOPACHROME DECARBOXYLASE; 1. DR PANTHER; PTHR11954:SF6; MACROPHAGE MIGRATION INHIBITORY FACTOR; 1. DR Pfam; PF01187; MIF; 1. DR SUPFAM; SSF55331; Tautomerase/MIF; 1. PE 1: Evidence at protein level; KW 3D-structure {ECO:0007829|PDB:3T5S}; KW Cytokine {ECO:0000256|ARBA:ARBA00022514}; KW Reference proteome {ECO:0000313|Proteomes:UP000001548}; KW Secreted {ECO:0000256|ARBA:ARBA00022525}. SQ SEQUENCE 114 AA; 12368 MW; C7BDC762C6B2E3FA CRC64; MPCAIVTTNA DFTKDQADAF CLDMGQVLAK ETGKPVSYCM AGVRKADMSF GTSTDLCCFV DFYCIGVISQ AKNPSISAAI TGCLTQHFKV KPERVYISFN EAKGHNWGFN GSTF //