ID A7L5W2_9HYPH Unreviewed; 523 AA. AC A7L5W2; DT 11-SEP-2007, integrated into UniProtKB/TrEMBL. DT 11-SEP-2007, sequence version 1. DT 22-FEB-2023, entry version 55. DE RecName: Full=DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00012418}; DE EC=2.7.7.6 {ECO:0000256|ARBA:ARBA00012418}; DE Flags: Fragment; GN Name=rpoB {ECO:0000313|EMBL:ABS52576.1}; OS Bartonella tamiae. OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; OC Bartonellaceae; Bartonella. OX NCBI_TaxID=373638 {ECO:0000313|EMBL:ABS52576.1}; RN [1] {ECO:0000313|EMBL:ABS52576.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Th339 {ECO:0000313|EMBL:ABS52576.1}; RX PubMed=18077632; DOI=10.1128/JCM.02120-07; RA Kosoy M., Morway C., Sheff K.W., Bai Y., Colborn J., Chalcraft L., RA Dowell S.F., Peruski L.F., Maloney S.A., Baggett H., Sutthirattana S., RA Sidhirat A., Maruyama S., Kabeya H., Chomel B.B., Kasten R., Popov V., RA Robinson J., Kruglov A., Petersen L.R.; RT "Bartonella tamiae sp. nov., a newly recognized pathogen isolated from RT three human patients from Thailand."; RL J. Clin. Microbiol. 46:772-775(2008). CC -!- CATALYTIC ACTIVITY: CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA- CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550}; CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family. CC {ECO:0000256|RuleBase:RU000434}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; EF672730; ABS52576.1; -; Genomic_DNA. DR AlphaFoldDB; A7L5W2; -. DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW. DR GO; GO:0003677; F:DNA binding; IEA:InterPro. DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC. DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro. DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro. DR CDD; cd00653; RNA_pol_B_RPB2; 1. DR Gene3D; 2.40.50.100; -; 1. DR Gene3D; 2.40.50.150; -; 1. DR Gene3D; 3.90.1100.10; -; 1. DR Gene3D; 2.30.150.10; DNA-directed RNA polymerase, beta subunit, external 1 domain; 1. DR Gene3D; 3.90.1110.10; RNA polymerase Rpb2, domain 2; 1. DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf. DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1. DR InterPro; IPR015712; DNA-dir_RNA_pol_su2. DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom. DR InterPro; IPR010243; RNA_pol_bsu_bac. DR InterPro; IPR007642; RNA_pol_Rpb2_2. DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf. DR InterPro; IPR007645; RNA_pol_Rpb2_3. DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold. DR PANTHER; PTHR20856; DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2; 1. DR PANTHER; PTHR20856:SF20; DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA; 1. DR Pfam; PF04561; RNA_pol_Rpb2_2; 1. DR Pfam; PF04565; RNA_pol_Rpb2_3; 1. DR Pfam; PF10385; RNA_pol_Rpb2_45; 1. DR Pfam; PF00562; RNA_pol_Rpb2_6; 1. DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1. DR TIGRFAMs; TIGR02013; rpoB; 1. PE 3: Inferred from homology; KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478}; KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695}; KW Transcription {ECO:0000256|ARBA:ARBA00023163}; KW Transferase {ECO:0000256|ARBA:ARBA00022679}. FT DOMAIN 7..68 FT /note="RNA polymerase Rpb2" FT /evidence="ECO:0000259|Pfam:PF04561" FT DOMAIN 127..195 FT /note="RNA polymerase Rpb2" FT /evidence="ECO:0000259|Pfam:PF04565" FT DOMAIN 205..270 FT /note="DNA-directed RNA polymerase beta subunit external 1" FT /evidence="ECO:0000259|Pfam:PF10385" FT DOMAIN 332..522 FT /note="DNA-directed RNA polymerase subunit 2 hybrid- FT binding" FT /evidence="ECO:0000259|Pfam:PF00562" FT NON_TER 1 FT /evidence="ECO:0000313|EMBL:ABS52576.1" FT NON_TER 523 FT /evidence="ECO:0000313|EMBL:ABS52576.1" SQ SEQUENCE 523 AA; 57962 MW; 30B9E9C49AD3582E CRC64; SLFFDPERYD LSAVGRVKMN MRMDLDCPDT VRILRKEDIL AVVKMLVELR DGRGEIDDID NLGNRRVRSV GELMENQYRV GLLRMERAIK ERMSSIEIDT VMPQDLINAK PAAAAVREFF GSSQLSQFMD QTNPLSEITH KRRLSALGPG GLTRERAGFE VRDVHPTHYG RICPIETPEG PNIGLINSLA TFARVNKYGF IESPYRKIVD GKVTTDVVYL SAMEESKHYV AQANSSLDAQ ARFTEEFVVC RHAGEVLMAP RDHVDLMDVS PKQLVSVAAA LIPFLENDDA NRALMGSNMQ RQAVPLVRAE APFVGTGMES VVARDSGAAI GARRAGIVDQ VDATRIVIHA TEDLDPTKSG VDIYRLQKFQ RSNQSTCINQ RPLVRVGDRI EKGDIIADGP STDLGDLALG RNVLVAFMPW NGYNYEDSIL LSERIVADDV FTSIHIEEFE VAARDTKLGP EEITRDIPNV SEEALKNLDE AGIVYIGAEV QPGDILVGKI TPKGESPMTP EEKLLRAIFG KKL //