ID TREPH_PLEPU Reviewed; 739 AA. AC A6YRN9; DT 08-MAR-2011, integrated into UniProtKB/Swiss-Prot. DT 21-AUG-2007, sequence version 1. DT 02-DEC-2020, entry version 19. DE RecName: Full=Trehalose phosphorylase {ECO:0000312|EMBL:ABR88135.1}; DE EC=2.4.1.231; DE AltName: Full=Trehalose synthase {ECO:0000250|UniProtKB:O75003}; DE Short=TSase {ECO:0000250|UniProtKB:O75003}; DE Flags: Precursor; GN Name=TP {ECO:0000250|UniProtKB:Q9UV63}; OS Pleurotus pulmonarius (Indian oyster mushroom). OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Agaricomycetidae; Agaricales; Pleurotaceae; Pleurotus. OX NCBI_TaxID=28995; RN [1] {ECO:0000312|EMBL:ABR88135.1} RP NUCLEOTIDE SEQUENCE [MRNA]. RA Tian Z.D., Xie C.H., Liu J.; RT "Isolation and functional characterization of trehalose phosphorylase gene RT in Pleurotus pulmonarius (Fr.)."; RL Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Reversibly catalyzes the synthesis and degradation of CC trehalose from glucose and alpha-D-glucose 1-phosphate. The equilibrium CC lies in the direction of trehalose synthesis (By similarity). CC {ECO:0000250|UniProtKB:O75003, ECO:0000250|UniProtKB:Q9UV63}. CC -!- CATALYTIC ACTIVITY: CC Reaction=alpha,alpha-trehalose + phosphate = alpha-D-glucose + alpha-D- CC glucose 1-phosphate; Xref=Rhea:RHEA:16257, ChEBI:CHEBI:16551, CC ChEBI:CHEBI:17925, ChEBI:CHEBI:43474, ChEBI:CHEBI:58601; CC EC=2.4.1.231; Evidence={ECO:0000250|UniProtKB:Q9UV63}; CC -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:O75003}. CC -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family. CC Glycosyltransferase 4 subfamily. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; EF645805; ABR88135.1; -; mRNA. DR CAZy; GT4; Glycosyltransferase Family 4. DR GO; GO:0033832; F:alpha,alpha-trehalose phosphorylase (configuration-retaining) activity; ISS:UniProtKB. DR GO; GO:0051156; P:glucose 6-phosphate metabolic process; ISS:UniProtKB. DR GO; GO:0006006; P:glucose metabolic process; ISS:UniProtKB. DR GO; GO:0005991; P:trehalose metabolic process; ISS:UniProtKB. DR InterPro; IPR001296; Glyco_trans_1. DR Pfam; PF00534; Glycos_transf_1; 1. PE 2: Evidence at transcript level; KW Carbohydrate metabolism; Glucose metabolism; Glycosyltransferase; KW Transferase. FT PROPEP 1..26 FT /evidence="ECO:0000250|UniProtKB:O75003" FT /id="PRO_0000405295" FT CHAIN 27..739 FT /note="Trehalose phosphorylase" FT /evidence="ECO:0000250|UniProtKB:O75003" FT /id="PRO_0000405296" SQ SEQUENCE 739 AA; 82028 MW; 6A8444D92D48EA95 CRC64; MSTPHHQFES KSSTAIRRRL SSSVSSKQRP NIMTTTFASL TPMWAGVAGT LVNNNTQYEI AVTVHDGVYS TDFASVIIPV TPGDTVKNSK DIEAQVLNLI RKFSAEHLCK FLGAGITLAL LKECPNLCTR LWLDMDIVPI VFNIKPFHTD SVTRPNIKHR ISSTTGSYVP SGSETPTVYV EASHLDDPSH LSPNAAQKLP IPRTLDEQSD SAARKCLMYF GPNNNPRLSI GARNQVTVDA GGKIHLIDDL EEYRETVGAG TWNAVIKLAD ELREKKVKIG FFSSTPQGGG VALMRHALIR FLTALDVDVA WYVPNPSPQV FRTTKNNHNI LQGVAAPDLR LTQEAKDAFD AWILKNGLRW TAEGGPLAPG GVDVVFIDDP QMPGLIPLIK KVRPEVPIVY RSHIEIRSDL VHVAGSPQEE VWKYLWNNIQ LADLFISHPV SKFVPSDVPT EKLALLGAAT DWLDGLNKDL DPWDSQFYMG EFRSPCAKEK MHELNWPARD YIVQVARFDP SKGIPNVVDS YYKFRNLLRT RSPDMDESEH PQLLICGHGA VDDPDASIIY DQIMALVNSD PYKEYAHDIV VMRLPPSDEL LNAMMANSRI ALQLSTREGF EVKVSEALHT GKPVIACRTG GIPLQIQHGK SGYLTTPGDN DAVAGHLYDL YTDEALYRKM SDFARTHVSD EVGTVGNAAA WLYLAVMYSR GEKIKPNGAW INDLLREETG EPYKEGETKL PRTKLDMQG //