ID GUAAA_META3 Reviewed; 188 AA. AC A6UVC9; DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot. DT 21-AUG-2007, sequence version 1. DT 24-JAN-2024, entry version 82. DE RecName: Full=GMP synthase [glutamine-hydrolyzing] subunit A {ECO:0000255|HAMAP-Rule:MF_01510}; DE EC=6.3.5.2 {ECO:0000255|HAMAP-Rule:MF_01510}; DE AltName: Full=Glutamine amidotransferase {ECO:0000255|HAMAP-Rule:MF_01510}; GN Name=guaAA {ECO:0000255|HAMAP-Rule:MF_01510}; OrderedLocusNames=Maeo_0869; OS Methanococcus aeolicus (strain ATCC BAA-1280 / DSM 17508 / OCM 812 / OS Nankai-3). OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales; OC Methanococcaceae; Methanococcus. OX NCBI_TaxID=419665; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC BAA-1280 / DSM 17508 / OCM 812 / Nankai-3; RG US DOE Joint Genome Institute; RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., RA Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., RA Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., RA Sieprawska-Lupa M., Whitman W.B., Richardson P.; RT "Complete sequence of Methanococcus aeolicus Nankai-3."; RL Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Catalyzes the synthesis of GMP from XMP. {ECO:0000255|HAMAP- CC Rule:MF_01510}. CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + H2O + L-glutamine + XMP = AMP + diphosphate + GMP + 2 CC H(+) + L-glutamate; Xref=Rhea:RHEA:11680, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:33019, ChEBI:CHEBI:57464, ChEBI:CHEBI:58115, CC ChEBI:CHEBI:58359, ChEBI:CHEBI:456215; EC=6.3.5.2; CC Evidence={ECO:0000255|HAMAP-Rule:MF_01510}; CC -!- PATHWAY: Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln CC route): step 1/1. {ECO:0000255|HAMAP-Rule:MF_01510}. CC -!- SUBUNIT: Heterodimer composed of a glutamine amidotransferase subunit CC (A) and a GMP-binding subunit (B). {ECO:0000255|HAMAP-Rule:MF_01510}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP000743; ABR56451.1; -; Genomic_DNA. DR RefSeq; WP_011973583.1; NC_009635.1. DR AlphaFoldDB; A6UVC9; -. DR SMR; A6UVC9; -. DR STRING; 419665.Maeo_0869; -. DR GeneID; 75305958; -. DR KEGG; mae:Maeo_0869; -. DR eggNOG; arCOG00087; Archaea. DR HOGENOM; CLU_014340_1_4_2; -. DR OMA; GPDMDRI; -. DR OrthoDB; 10772at2157; -. DR UniPathway; UPA00189; UER00296. DR Proteomes; UP000001106; Chromosome. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0003922; F:GMP synthase (glutamine-hydrolyzing) activity; IEA:UniProtKB-UniRule. DR GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW. DR CDD; cd01742; GATase1_GMP_Synthase; 1. DR Gene3D; 3.40.50.880; -; 1. DR HAMAP; MF_01510; GMP_synthase_A; 1. DR InterPro; IPR029062; Class_I_gatase-like. DR InterPro; IPR017926; GATASE. DR InterPro; IPR004739; GMP_synth_GATase. DR InterPro; IPR023686; GMP_synthase_A. DR NCBIfam; TIGR00888; guaA_Nterm; 1. DR PANTHER; PTHR11922:SF2; GMP SYNTHASE [GLUTAMINE-HYDROLYZING]; 1. DR PANTHER; PTHR11922; GMP SYNTHASE-RELATED; 1. DR Pfam; PF00117; GATase; 1. DR PRINTS; PR00097; ANTSNTHASEII. DR PRINTS; PR00096; GATASE. DR SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1. DR PROSITE; PS51273; GATASE_TYPE_1; 1. PE 3: Inferred from homology; KW ATP-binding; Glutamine amidotransferase; GMP biosynthesis; Ligase; KW Nucleotide-binding; Purine biosynthesis. FT CHAIN 1..188 FT /note="GMP synthase [glutamine-hydrolyzing] subunit A" FT /id="PRO_0000316093" FT DOMAIN 1..188 FT /note="Glutamine amidotransferase type-1" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01510" FT ACT_SITE 76 FT /note="Nucleophile" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01510" FT ACT_SITE 163 FT /evidence="ECO:0000255|HAMAP-Rule:MF_01510" FT ACT_SITE 165 FT /evidence="ECO:0000255|HAMAP-Rule:MF_01510" SQ SEQUENCE 188 AA; 20877 MW; 2540A7E6E98378CC CRC64; MIVILNNGGQ YVHRIHRSLR YLKIPSKIIP NSTPLAEIEE NKEIKGIILS GGPDIEKASN CLDIALNSKL PILGICLGHQ IIAKAYGGEI GRAESEEYAH SKIFVKEEND LFKNVPKEFT AWASHKDEVV GAPLNFEILA YSNICEVEAM KHKEKPIYGV QFHPEVSHTE NGAEILKNFC KVCGLLGE //