ID A6JW63_RAT Unreviewed; 163 AA. AC A6JW63; DT 28-JUN-2023, integrated into UniProtKB/TrEMBL. DT 28-JUN-2023, sequence version 1. DT 02-OCT-2024, entry version 7. DE RecName: Full=2-acylglycerol O-acyltransferase {ECO:0000256|ARBA:ARBA00039151}; DE EC=2.3.1.22 {ECO:0000256|ARBA:ARBA00039151}; GN Name=Mogat1_predicted {ECO:0000313|EMBL:EDL75472.1}; GN ORFNames=rCG_23914 {ECO:0000313|EMBL:EDL75472.1}; OS Rattus norvegicus (Rat). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Rattus. OX NCBI_TaxID=10116 {ECO:0000313|EMBL:EDL75472.1, ECO:0000313|Proteomes:UP000234681}; RN [1] {ECO:0000313|Proteomes:UP000234681} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=BN, Sprague-Dawley {ECO:0000313|Proteomes:UP000234681}; RA Mural R.J., Li P.W., Adams M.D., Amanatides P.G., Baden-Tillson H., RA Barnstead M., Chin S.H., Dew I., Evans C.A., Ferriera S., Flanigan M., RA Fosler C., Glodek A., Gu Z., Holt R.A., Jennings D., Kraft C.L., Lu F., RA Nguyen T., Nusskern D.R., Pfannkoch C.M., Sitter C., Sutton G.G., RA Venter J.C., Wang Z., Woodage T., Zheng X.H., Zhong F.; RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=a 1-acylglycerol + an acyl-CoA = a 1,2-diacylglycerol + CoA; CC Xref=Rhea:RHEA:39943, ChEBI:CHEBI:35759, ChEBI:CHEBI:49172, CC ChEBI:CHEBI:57287, ChEBI:CHEBI:58342; CC Evidence={ECO:0000256|ARBA:ARBA00043696}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:39944; CC Evidence={ECO:0000256|ARBA:ARBA00043696}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 1-acylglycerol + an acyl-CoA = a 1,3-diacylglycerol + CoA; CC Xref=Rhea:RHEA:77571, ChEBI:CHEBI:35759, ChEBI:CHEBI:47777, CC ChEBI:CHEBI:57287, ChEBI:CHEBI:58342; CC Evidence={ECO:0000256|ARBA:ARBA00043685}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:77572; CC Evidence={ECO:0000256|ARBA:ARBA00043685}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 2-acylglycerol + an acyl-CoA = a 1,2-diacyl-sn-glycerol + CC CoA; Xref=Rhea:RHEA:32947, ChEBI:CHEBI:17389, ChEBI:CHEBI:17815, CC ChEBI:CHEBI:57287, ChEBI:CHEBI:58342; CC Evidence={ECO:0000256|ARBA:ARBA00043656}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:32948; CC Evidence={ECO:0000256|ARBA:ARBA00043656}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 2-acylglycerol + an acyl-CoA = a 1,2-diacylglycerol + CoA; CC Xref=Rhea:RHEA:16741, ChEBI:CHEBI:17389, ChEBI:CHEBI:49172, CC ChEBI:CHEBI:57287, ChEBI:CHEBI:58342; EC=2.3.1.22; CC Evidence={ECO:0000256|ARBA:ARBA00043704}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16742; CC Evidence={ECO:0000256|ARBA:ARBA00043704}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 2-acylglycerol + an acyl-CoA = a 2,3-diacyl-sn-glycerol + CC CoA; Xref=Rhea:RHEA:38467, ChEBI:CHEBI:17389, ChEBI:CHEBI:57287, CC ChEBI:CHEBI:58342, ChEBI:CHEBI:75524; CC Evidence={ECO:0000256|ARBA:ARBA00043663}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38468; CC Evidence={ECO:0000256|ARBA:ARBA00043663}; CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis. CC {ECO:0000256|ARBA:ARBA00004771}. CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}. CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane CC {ECO:0000256|ARBA:ARBA00004477}; Multi-pass membrane protein CC {ECO:0000256|ARBA:ARBA00004477}. Membrane CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein CC {ECO:0000256|ARBA:ARBA00004141}. CC -!- SIMILARITY: Belongs to the diacylglycerol acyltransferase family. CC {ECO:0000256|ARBA:ARBA00005420}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CH474004; EDL75472.1; -; Genomic_DNA. DR Proteomes; UP000234681; Chromosome 9. DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell. DR GO; GO:0008374; F:O-acyltransferase activity; IEA:InterPro. DR GO; GO:0044255; P:cellular lipid metabolic process; IEA:UniProt. DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW. DR InterPro; IPR007130; DAGAT. DR PANTHER; PTHR12317:SF26; 2-ACYLGLYCEROL O-ACYLTRANSFERASE 1; 1. DR PANTHER; PTHR12317; DIACYLGLYCEROL O-ACYLTRANSFERASE; 1. DR Pfam; PF03982; DAGAT; 1. PE 3: Inferred from homology; KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315, KW ECO:0000313|EMBL:EDL75472.1}; KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824}; KW Glycerol metabolism {ECO:0000256|ARBA:ARBA00022798}; KW Membrane {ECO:0000256|ARBA:ARBA00023136}; KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:EDL75472.1}; KW Transmembrane {ECO:0000256|ARBA:ARBA00022692}; KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989}. SQ SEQUENCE 163 AA; 18449 MW; A1179EA4A9B48103 CRC64; MSKEGGGNIS VIVIGGAKEL LESFPGRYSL CLLQRKGFVK IALTHGAHLV PVFSFGENEL YSQVDNPKGS WLRTAQDKVY NLTGLALPLF YARGIFQNSF GLMPYRKLIY TVVGRPIPVQ KTLHPTPEQI DELHQTYMEE LRKLFNEHKG KYGIPEHETL VFK //