ID TRUD_HAEIE Reviewed; 339 AA. AC A5UE25; DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot. DT 10-JUL-2007, sequence version 1. DT 26-FEB-2020, entry version 72. DE RecName: Full=tRNA pseudouridine synthase D {ECO:0000255|HAMAP-Rule:MF_01082}; DE EC=5.4.99.27 {ECO:0000255|HAMAP-Rule:MF_01082}; DE AltName: Full=tRNA pseudouridine(13) synthase {ECO:0000255|HAMAP-Rule:MF_01082}; DE AltName: Full=tRNA pseudouridylate synthase D {ECO:0000255|HAMAP-Rule:MF_01082}; DE AltName: Full=tRNA-uridine isomerase D {ECO:0000255|HAMAP-Rule:MF_01082}; GN Name=truD {ECO:0000255|HAMAP-Rule:MF_01082}; GN OrderedLocusNames=CGSHiEE_08635; OS Haemophilus influenzae (strain PittEE). OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; OC Pasteurellaceae; Haemophilus. OX NCBI_TaxID=374930; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=PittEE; RX PubMed=17550610; DOI=10.1186/gb-2007-8-6-r103; RA Hogg J.S., Hu F.Z., Janto B., Boissy R., Hayes J., Keefe R., Post J.C., RA Ehrlich G.D.; RT "Characterization and modeling of the Haemophilus influenzae core and RT supragenomes based on the complete genomic sequences of Rd and 12 clinical RT nontypeable strains."; RL Genome Biol. 8:R103.1-R103.18(2007). CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-13 in CC transfer RNAs. {ECO:0000255|HAMAP-Rule:MF_01082}. CC -!- CATALYTIC ACTIVITY: CC Reaction=uridine(13) in tRNA = pseudouridine(13) in tRNA; CC Xref=Rhea:RHEA:42540, Rhea:RHEA-COMP:10105, Rhea:RHEA-COMP:10106, CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.27; CC Evidence={ECO:0000255|HAMAP-Rule:MF_01082}; CC -!- SIMILARITY: Belongs to the pseudouridine synthase TruD family. CC {ECO:0000255|HAMAP-Rule:MF_01082}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP000671; ABQ99026.1; -; Genomic_DNA. DR RefSeq; WP_012054625.1; NC_009566.1. DR SMR; A5UE25; -. DR EnsemblBacteria; ABQ99026; ABQ99026; CGSHiEE_08635. DR KEGG; hip:CGSHiEE_08635; -. DR HOGENOM; CLU_005281_4_0_6; -. DR KO; K06176; -. DR OMA; LWLWVEK; -. DR BioCyc; HINF374930:G1G8O-1674-MONOMER; -. DR GO; GO:0003723; F:RNA binding; IEA:InterPro. DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC. DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule. DR Gene3D; 3.30.2350.20; -; 1. DR HAMAP; MF_01082; TruD; 1. DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf. DR InterPro; IPR001656; PsdUridine_synth_TruD. DR InterPro; IPR020119; PsdUridine_synth_TruD_CS. DR InterPro; IPR011760; PsdUridine_synth_TruD_insert. DR InterPro; IPR042214; TruD_catalytic. DR Pfam; PF01142; TruD; 2. DR SUPFAM; SSF55120; SSF55120; 1. DR TIGRFAMs; TIGR00094; tRNA_TruD_broad; 1. DR PROSITE; PS50984; TRUD; 1. DR PROSITE; PS01268; UPF0024; 1. PE 3: Inferred from homology; KW Isomerase; tRNA processing. FT CHAIN 1..339 FT /note="tRNA pseudouridine synthase D" FT /id="PRO_1000084742" FT DOMAIN 155..311 FT /note="TRUD" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01082" FT ACT_SITE 80 FT /note="Nucleophile" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01082" SQ SEQUENCE 339 AA; 38503 MW; 9C5393F3F01CA3A2 CRC64; MLEQLPYLAL KTPPKTTALL KAECADFIVK EHLGYEMSGD GEFVALYVRK TDCNTLFVGE KLAKFAGVSE RNMGYAGLKD RRAVTEQWFC LQMPGMETPD FSQFELDGVE ILTVTRHNRK IRTGSLEGNY FDILLRGAEE SDELKVRLDF VANFGFPNYF TEQRFGREGH NLTQALRWAQ GEIKVKDRKK RSFYLSAARS EIFNLVVAAR IAKGATNQVL PNDIVQLAGS HSWFKADEKE DLNALQVRLE NQDILLTAPL IGEDILAASD IENEIVNQHS VFDPLMKQER MKAARRPLLM KAKGFSWAFE LEGLRLKFYL PAGSYATALV RELVNYTEE //