ID AMPA_LEGPC Reviewed; 483 AA. AC A5IAU5; DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot. DT 26-JUN-2007, sequence version 1. DT 01-MAY-2013, entry version 44. DE RecName: Full=Probable cytosol aminopeptidase; DE EC=3.4.11.1; DE AltName: Full=Leucine aminopeptidase; DE Short=LAP; DE EC=3.4.11.10; DE AltName: Full=Leucyl aminopeptidase; GN Name=pepA; OrderedLocusNames=LPC_0510; OS Legionella pneumophila (strain Corby). OC Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; OC Legionellaceae; Legionella. OX NCBI_TaxID=400673; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Corby; RA Gloeckner G., Albert-Weissenberger C., Weinmann E., Jacobi S., RA Schunder E., Steinert M., Buchrieser C., Hacker J., Heuner K.; RT "Identification and characterization of a new conjugation/ type IVA RT secretion system (trb/tra) of L. pneumophila Corby localized on a RT mobile genomic island."; RL Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Presumably involved in the processing and regular CC turnover of intracellular proteins. Catalyzes the removal of CC unsubstituted N-terminal amino acids from various peptides (By CC similarity). CC -!- CATALYTIC ACTIVITY: Release of an N-terminal amino acid, Xaa-|- CC Yaa-, in which Xaa is preferably Leu, but may be other amino acids CC including Pro although not Arg or Lys, and Yaa may be Pro. Amino CC acid amides and methyl esters are also readily hydrolyzed, but CC rates on arylamides are exceedingly low. CC -!- CATALYTIC ACTIVITY: Release of an N-terminal amino acid, CC preferentially leucine, but not glutamic or aspartic acids. CC -!- COFACTOR: Binds 2 manganese ions per subunit (By similarity). CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity). CC -!- SIMILARITY: Belongs to the peptidase M17 family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP000675; ABQ54495.1; -; Genomic_DNA. DR RefSeq; YP_001249841.1; NC_009494.2. DR ProteinModelPortal; A5IAU5; -. DR STRING; 400673.LPC_0510; -. DR MEROPS; M17.003; -. DR EnsemblBacteria; ABQ54495; ABQ54495; LPC_0510. DR GeneID; 5182305; -. DR KEGG; lpc:LPC_0510; -. DR PATRIC; 22307385; VBILegPne45588_0530. DR eggNOG; COG0260; -. DR HOGENOM; HOG000243132; -. DR KO; K01255; -. DR OMA; MPANICT; -. DR ProtClustDB; CLSK833523; -. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0004177; F:aminopeptidase activity; IEA:HAMAP. DR GO; GO:0030145; F:manganese ion binding; IEA:HAMAP. DR GO; GO:0008235; F:metalloexopeptidase activity; IEA:HAMAP. DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW. DR HAMAP; MF_00181; Cytosol_peptidase_M17; 1; -. DR InterPro; IPR011356; Leucine_aapep/pepB. DR InterPro; IPR000819; Peptidase_M17_C. DR InterPro; IPR023042; Peptidase_M17_leu_NH2_pept. DR InterPro; IPR008283; Peptidase_M17_N. DR PANTHER; PTHR11963:SF3; PTHR11963:SF3; 1. DR Pfam; PF00883; Peptidase_M17; 1. DR Pfam; PF02789; Peptidase_M17_N; 1. DR PRINTS; PR00481; LAMNOPPTDASE. DR PROSITE; PS00631; CYTOSOL_AP; 1. PE 3: Inferred from homology; KW Aminopeptidase; Complete proteome; Cytoplasm; Hydrolase; Manganese; KW Metal-binding; Protease. FT CHAIN 1 483 Probable cytosol aminopeptidase. FT /FTId=PRO_1000203833. FT ACT_SITE 264 264 Potential. FT ACT_SITE 338 338 Potential. FT METAL 252 252 Manganese 2 (By similarity). FT METAL 257 257 Manganese 1 (By similarity). FT METAL 257 257 Manganese 2 (By similarity). FT METAL 275 275 Manganese 2 (By similarity). FT METAL 334 334 Manganese 1 (By similarity). FT METAL 336 336 Manganese 1 (By similarity). FT METAL 336 336 Manganese 2 (By similarity). SQ SEQUENCE 483 AA; 52613 MW; 695CC2F46D38ACE6 CRC64; MNYGLTHTPS LTMSECLVLG VFSDLALPDF ATAIDNEQKG LIKKLCQRVP EPGNTVWQTD VEGHSLLIIQ CGKKEEFSAN SLQKRVGEIT EALIKQRFSS ATVCLPRLNQ ESAEWQLEQM IVQIDNLRYQ LLDFKTKHAK SHKLESVIFH LPGATEKSLE MAKAIVTGVE FCRDLANMPA NICTPTYLGE QAISLSKQFD QISCQVMGPE EIKEMGMGAL LAVAQGSDQP PRLIDIHYHG NKNSTPVILV GKGITFDSGG LSIKPANAMD EMKYDMSGAA SVLGVIKACA LLKLPINLIG IIASAENLIS GSAVKSGDIV TTMSGQTVEI INTDAEGRLV LADALTYAER YNPDFVIDIA TLTGAIIVAL GNIATGYMTR DEQLAKSIER AANESQDKVW RMPLDEAYQD ALESPLADMI NAGFDRSAGS ITAACFLSRF TEKYRWAHLD IAGTAWISGK KRNATGRPVP LLIQLLRHVA NSR //