ID DEF2_PINSY Reviewed; 83 AA. AC A4L7R8; DT 23-MAR-2010, integrated into UniProtKB/Swiss-Prot. DT 01-MAY-2007, sequence version 1. DT 27-MAR-2024, entry version 29. DE RecName: Full=Defensin-2; DE Flags: Precursor; GN Name=Def2 {ECO:0000312|EMBL:ABO61349.1}; OS Pinus sylvestris (Scotch pine). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Pinus; OC Pinus subgen. Pinus. OX NCBI_TaxID=3349; RN [1] {ECO:0000312|EMBL:ABO61349.1} RP NUCLEOTIDE SEQUENCE [MRNA]. RC TISSUE=Root {ECO:0000312|EMBL:ABO61349.1}; RX PubMed=19253756; RA Koval'ova V.A., Hut R.T.; RT "Molecular cloning and characterization of Scotch pine Defensin-2."; RL Cyt. Genet. 42:55-60(2008). CC -!- FUNCTION: Plant defense peptide. Has antifungal activity. CC {ECO:0000250|UniProtKB:A4L7R7}. CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q43413}. CC -!- SIMILARITY: Belongs to the DEFL family. {ECO:0000255}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; EF455617; ABO61349.1; -; mRNA. DR PDB; 7LNS; NMR; -; A=34-83. DR PDBsum; 7LNS; -. DR AlphaFoldDB; A4L7R8; -. DR SMR; A4L7R8; -. DR GO; GO:0005576; C:extracellular region; ISS:UniProtKB. DR GO; GO:0050832; P:defense response to fungus; ISS:UniProtKB. DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW. DR CDD; cd00107; Knot1; 1. DR Gene3D; 3.30.30.10; Knottin, scorpion toxin-like; 1. DR InterPro; IPR008176; Defensin_plant. DR InterPro; IPR003614; Scorpion_toxin-like. DR InterPro; IPR036574; Scorpion_toxin-like_sf. DR PANTHER; PTHR33147; DEFENSIN-LIKE PROTEIN 1; 1. DR PANTHER; PTHR33147:SF165; DRO1 PROTEIN-RELATED; 1. DR Pfam; PF00304; Gamma-thionin; 1. DR PRINTS; PR00288; PUROTHIONIN. DR SMART; SM00505; Knot1; 1. DR SUPFAM; SSF57095; Scorpion toxin-like; 1. DR PROSITE; PS00940; GAMMA_THIONIN; 1. PE 1: Evidence at protein level; KW 3D-structure; Antimicrobial; Defensin; Disulfide bond; Fungicide; Secreted; KW Signal. FT SIGNAL 1..33 FT /evidence="ECO:0000255" FT CHAIN 34..83 FT /note="Defensin-2" FT /id="PRO_0000392922" FT DISULFID 36..82 FT /evidence="ECO:0000250|UniProtKB:P32026" FT DISULFID 47..67 FT /evidence="ECO:0000250|UniProtKB:P32026" FT DISULFID 53..76 FT /evidence="ECO:0000250|UniProtKB:P32026" FT DISULFID 57..78 FT /evidence="ECO:0000250|UniProtKB:P32026" FT STRAND 35..39 FT /evidence="ECO:0007829|PDB:7LNS" FT HELIX 50..60 FT /evidence="ECO:0007829|PDB:7LNS" FT STRAND 63..67 FT /evidence="ECO:0007829|PDB:7LNS" FT STRAND 71..74 FT /evidence="ECO:0007829|PDB:7LNS" FT STRAND 76..81 FT /evidence="ECO:0007829|PDB:7LNS" SQ SEQUENCE 83 AA; 8963 MW; CE44E1A01E3731B6 CRC64; MAGKGVGTPL SALFLLVLLV VTIGMMEVQV AEGRMCKTPS AKFKGYCVSS TNCKNVCRTE GFPTGSCDFH ITSRKCYCYK PCP //