ID MURB_DESRM Reviewed; 303 AA. AC A4J2B3; DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot. DT 01-MAY-2007, sequence version 1. DT 01-MAY-2013, entry version 51. DE RecName: Full=UDP-N-acetylenolpyruvoylglucosamine reductase; DE EC=1.3.1.98; DE AltName: Full=UDP-N-acetylmuramate dehydrogenase; GN Name=murB; OrderedLocusNames=Dred_0677; OS Desulfotomaculum reducens (strain MI-1). OC Bacteria; Firmicutes; Clostridia; Clostridiales; Peptococcaceae; OC Desulfotomaculum. OX NCBI_TaxID=349161; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=MI-1; RG US DOE Joint Genome Institute; RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., RA Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., RA Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., RA Tebo B.M., Richardson P.; RT "Complete sequence of Desulfotomaculum reducens MI-1."; RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Cell wall formation (By similarity). CC -!- CATALYTIC ACTIVITY: UDP-N-acetyl-alpha-D-muramate + NADP(+) = UDP- CC N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine + NADPH. CC -!- COFACTOR: FAD (By similarity). CC -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis. CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity). CC -!- SIMILARITY: Belongs to the MurB family. CC -!- SIMILARITY: Contains 1 FAD-binding PCMH-type domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP000612; ABO49216.1; -; Genomic_DNA. DR RefSeq; YP_001112041.1; NC_009253.1. DR ProteinModelPortal; A4J2B3; -. DR STRING; 349161.Dred_0677; -. DR EnsemblBacteria; ABO49216; ABO49216; Dred_0677. DR GeneID; 4956648; -. DR KEGG; drm:Dred_0677; -. DR PATRIC; 21727325; VBIDesRed82656_0750. DR eggNOG; COG0812; -. DR HOGENOM; HOG000284355; -. DR KO; K00075; -. DR OMA; SKKHAGF; -. DR BioCyc; DRED349161:GHP6-776-MONOMER; -. DR UniPathway; UPA00219; -. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro. DR GO; GO:0008762; F:UDP-N-acetylmuramate dehydrogenase activity; IEA:HAMAP. DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW. DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW. DR GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:HAMAP. DR GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW. DR Gene3D; 3.30.43.10; -; 1. DR Gene3D; 3.30.465.10; -; 1. DR Gene3D; 3.90.78.10; -; 1. DR HAMAP; MF_00037; MurB; 1; -. DR InterPro; IPR016169; CO_DH_flavot_FAD-bd_sub2. DR InterPro; IPR016166; FAD-bd_2. DR InterPro; IPR016167; FAD-bd_2_sub1. DR InterPro; IPR003170; MurB. DR InterPro; IPR011601; MurB_C. DR InterPro; IPR006094; Oxid_FAD_bind_N. DR PANTHER; PTHR21071; PTHR21071; 1. DR Pfam; PF01565; FAD_binding_4; 1. DR Pfam; PF02873; MurB_C; 1. DR SUPFAM; SSF56176; FAD-binding_2; 1. DR SUPFAM; SSF56194; MurB_C; 1. DR TIGRFAMs; TIGR00179; murB; 1. DR PROSITE; PS51387; FAD_PCMH; 1. PE 3: Inferred from homology; KW Cell cycle; Cell division; Cell shape; KW Cell wall biogenesis/degradation; Complete proteome; Cytoplasm; FAD; KW Flavoprotein; NADP; Oxidoreductase; Peptidoglycan synthesis. FT CHAIN 1 303 UDP-N-acetylenolpyruvoylglucosamine FT reductase. FT /FTId=PRO_1000071038. FT DOMAIN 32 212 FAD-binding PCMH-type. FT ACT_SITE 176 176 By similarity. FT ACT_SITE 226 226 Proton donor (By similarity). FT ACT_SITE 296 296 By similarity. SQ SEQUENCE 303 AA; 32593 MW; 07AA6525BDA3E7D3 CRC64; MIYTSLAGEL QSLVKGSIQI NEPMRKHTTW KIGGKADLFL NPSDKEDIRQ AVEFAREKAI PITVIGNGSN LLVKDGGIRG LVIKVGRGMA KITIEGTSIK AGAGALLPEL AVFACKNSLG GFGFAAGIPG SLGGAIVMNA GAMNGCVSDV LQSIVVLNER NQFEVLTKDH LNFAYRTSNL QSRGLICVET CWQGYAKDQW LIEQETKEYL AKRKAAQPQG FPNAGSVFKN PEGDFAGRLI EGCGGKGLRV GDAEVSSKHA NWILNLGRAT AQDVLILIDH LKQMVQERFG VLLQLEVKVL GED //