ID A4FV12_BOVIN Unreviewed; 640 AA. AC A4FV12; DT 17-APR-2007, integrated into UniProtKB/TrEMBL. DT 17-APR-2007, sequence version 1. DT 22-NOV-2017, entry version 93. DE SubName: Full=UBA2 protein {ECO:0000313|EMBL:AAI23591.1}; DE SubName: Full=Ubiquitin like modifier activating enzyme 2 {ECO:0000313|Ensembl:ENSBTAP00000003333}; GN Name=UBA2 {ECO:0000313|EMBL:AAI23591.1, GN ECO:0000313|Ensembl:ENSBTAP00000003333}; OS Bos taurus (Bovine). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; OC Pecora; Bovidae; Bovinae; Bos. OX NCBI_TaxID=9913 {ECO:0000313|EMBL:AAI23591.1}; RN [1] {ECO:0000313|EMBL:AAI23591.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=Hereford {ECO:0000313|EMBL:AAI23591.1}; RC TISSUE=Fetal lung {ECO:0000313|EMBL:AAI23591.1}; RA Moore S., Alexander L., Brownstein M., Guan L., Lobo S., Meng Y., RA Tanaguchi M., Wang Z., Yu J., Prange C., Schreiber K., Shenmen C., RA Wagner L., Bala M., Barbazuk S., Barber S., Babakaiff R., Beland J., RA Chun E., Del Rio L., Gibson S., Hanson R., Kirkpatrick R., Liu J., RA Matsuo C., Mayo M., Santos R.R., Stott J., Tsai M., Wong D., RA Siddiqui A., Holt R., Jones S.J., Marra M.A.; RL Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|Ensembl:ENSBTAP00000003333, ECO:0000313|Proteomes:UP000009136} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Hereford {ECO:0000313|Ensembl:ENSBTAP00000003333, RC ECO:0000313|Proteomes:UP000009136}; RX PubMed=19393038; DOI=10.1186/gb-2009-10-4-r42; RA Zimin A.V., Delcher A.L., Florea L., Kelley D.R., Schatz M.C., RA Puiu D., Hanrahan F., Pertea G., Van Tassell C.P., Sonstegard T.S., RA Marcais G., Roberts M., Subramanian P., Yorke J.A., Salzberg S.L.; RT "A whole-genome assembly of the domestic cow, Bos taurus."; RL Genome Biol. 10:R42.01-R42.10(2009). RN [3] {ECO:0000313|Ensembl:ENSBTAP00000003333} RP IDENTIFICATION. RC STRAIN=Hereford {ECO:0000313|Ensembl:ENSBTAP00000003333}; RG Ensembl; RL Submitted (MAR-2016) to UniProtKB. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; DAAA02046904; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; BC123590; AAI23591.1; -; mRNA. DR RefSeq; NP_001076850.1; NM_001083381.1. DR UniGene; Bt.13185; -. DR STRING; 9913.ENSBTAP00000003333; -. DR Ensembl; ENSBTAT00000003333; ENSBTAP00000003333; ENSBTAG00000002573. DR GeneID; 507005; -. DR KEGG; bta:507005; -. DR CTD; 10054; -. DR eggNOG; KOG2013; Eukaryota. DR eggNOG; COG0476; LUCA. DR GeneTree; ENSGT00550000074924; -. DR HOGENOM; HOG000216514; -. DR HOVERGEN; HBG060266; -. DR KO; K10685; -. DR OMA; QKKHVGK; -. DR OrthoDB; EOG091G07PV; -. DR TreeFam; TF300765; -. DR Reactome; R-BTA-3065676; SUMO is conjugated to E1 (UBA2:SAE1). DR Reactome; R-BTA-3065678; SUMO is transferred from E1 to E2 (UBE2I, UBC9). DR Proteomes; UP000009136; Chromosome 18. DR Bgee; ENSBTAG00000002573; -. DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central. DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl. DR GO; GO:0031510; C:SUMO activating enzyme complex; IEA:Ensembl. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0000287; F:magnesium ion binding; IEA:Ensembl. DR GO; GO:0046982; F:protein heterodimerization activity; IEA:Ensembl. DR GO; GO:0044388; F:small protein activating enzyme binding; IEA:Ensembl. DR GO; GO:0019948; F:SUMO activating enzyme activity; IBA:GO_Central. DR GO; GO:0032183; F:SUMO binding; IEA:Ensembl. DR GO; GO:0008134; F:transcription factor binding; IEA:Ensembl. DR GO; GO:0044390; F:ubiquitin-like protein conjugating enzyme binding; IEA:Ensembl. DR GO; GO:0016925; P:protein sumoylation; IBA:GO_Central. DR InterPro; IPR000594; ThiF_NAD_FAD-bd. DR InterPro; IPR028077; UAE_UbL_dom. DR InterPro; IPR030661; Uba2. DR InterPro; IPR032426; UBA2_C. DR InterPro; IPR035985; Ubiquitin-activating_enz. DR InterPro; IPR018074; UBQ-activ_enz_E1_CS. DR InterPro; IPR033127; UBQ-activ_enz_E1_Cys_AS. DR PANTHER; PTHR10953:SF5; PTHR10953:SF5; 2. DR Pfam; PF00899; ThiF; 1. DR Pfam; PF14732; UAE_UbL; 1. DR Pfam; PF16195; UBA2_C; 1. DR PIRSF; PIRSF039133; SUMO_E1B; 1. DR SUPFAM; SSF69572; SSF69572; 2. DR PROSITE; PS00536; UBIQUITIN_ACTIVAT_1; 1. DR PROSITE; PS00865; UBIQUITIN_ACTIVAT_2; 1. PE 1: Evidence at protein level; KW ATP-binding {ECO:0000256|PIRSR:PIRSR039133-2}; KW Complete proteome {ECO:0000313|Proteomes:UP000009136}; KW Metal-binding {ECO:0000256|PIRSR:PIRSR039133-3}; KW Nucleotide-binding {ECO:0000256|PIRSR:PIRSR039133-2}; KW Proteomics identification {ECO:0000213|PeptideAtlas:A4FV12}; KW Reference proteome {ECO:0000313|Proteomes:UP000009136}; KW Zinc {ECO:0000256|PIRSR:PIRSR039133-3}. FT DOMAIN 10 433 ThiF. {ECO:0000259|Pfam:PF00899}. FT DOMAIN 452 537 UAE_UbL. {ECO:0000259|Pfam:PF14732}. FT DOMAIN 549 632 UBA2_C. {ECO:0000259|Pfam:PF16195}. FT NP_BIND 24 29 ATP. {ECO:0000256|PIRSR:PIRSR039133-2}. FT NP_BIND 56 59 ATP. {ECO:0000256|PIRSR:PIRSR039133-2}. FT NP_BIND 95 96 ATP. {ECO:0000256|PIRSR:PIRSR039133-2}. FT NP_BIND 117 122 ATP. {ECO:0000256|PIRSR:PIRSR039133-2}. FT ACT_SITE 173 173 Glycyl thioester intermediate. FT {ECO:0000256|PIRSR:PIRSR039133-1, FT ECO:0000256|PROSITE-ProRule:PRU10132}. FT METAL 158 158 Zinc. {ECO:0000256|PIRSR:PIRSR039133-3}. FT METAL 161 161 Zinc. {ECO:0000256|PIRSR:PIRSR039133-3}. FT METAL 441 441 Zinc. {ECO:0000256|PIRSR:PIRSR039133-3}. FT METAL 444 444 Zinc. {ECO:0000256|PIRSR:PIRSR039133-3}. FT BINDING 48 48 ATP. {ECO:0000256|PIRSR:PIRSR039133-2}. FT BINDING 72 72 ATP. {ECO:0000256|PIRSR:PIRSR039133-2}. FT CROSSLNK 164 164 Glycyl lysine isopeptide (Lys-Gly) FT (interchain with G-Cter in SUMO1). FT {ECO:0000256|PIRSR:PIRSR039133-4}. FT CROSSLNK 190 190 Glycyl lysine isopeptide (Lys-Gly) FT (interchain with G-Cter in SUMO). FT {ECO:0000256|PIRSR:PIRSR039133-4}. FT CROSSLNK 236 236 Glycyl lysine isopeptide (Lys-Gly) FT (interchain with G-Cter in SUMO2). FT {ECO:0000256|PIRSR:PIRSR039133-4}. FT CROSSLNK 257 257 Glycyl lysine isopeptide (Lys-Gly) FT (interchain with G-Cter in SUMO). FT {ECO:0000256|PIRSR:PIRSR039133-4}. FT CROSSLNK 420 420 Glycyl lysine isopeptide (Lys-Gly) FT (interchain with G-Cter in SUMO1). FT {ECO:0000256|PIRSR:PIRSR039133-4}. FT CROSSLNK 623 623 Glycyl lysine isopeptide (Lys-Gly) FT (interchain with G-Cter in SUMO). FT {ECO:0000256|PIRSR:PIRSR039133-4}. SQ SEQUENCE 640 AA; 71031 MW; 4B1946642E4AE5DA CRC64; MALSRGLPRE LAEAVAGGRV LVVGAGGIGC ELLKNLVLTG FSHIDLIDLD TIDVSNLNRQ FLFQKKHVGR SKAQVAKESV LQFYPKANIV AYHDSIMNPD YNVEFFRQFI LVMNALDNRA ARNHVNRMCL AADVPLIESG TAGYLGQVTT IKKGVTECYE CHPKPTQRTF PGCTIRNTPS EPIHCIVWAK YLFNQLFGEE DADQEVSPDR ADPEASWEPM EAEARARASN EDGDIKRVST KEWAKSTGYD PVKLFTKLFK DDIRYLLTMD KLWRKRKPPV PLDWAEVQSQ GEETSASDQQ NEPQLGLKDQ QVLDVKSYAC LFSKSIETLR VHLAEKGDGA ELIWDKDDPS AMDFVTSAAN LRMHIFSMNM KSRFDIKSMA GNIIPAIATT NAVIAGLIVL EGLKILSGKI DQCRTIFLNK QPNPRKKLLV PCALDAPNPN CYVCASKPEV TVRLNVHKVT VLTLQDKIVK EKFAMVAPDV QIEDGKGTIL ISSEEGETEA NNHKKLSEFG IRNGSRLQAD DFLQDYTLLI NILHSEDLGK DVEFEVVGDA PEKVGPKQAE DAAKSITNGS DDGAQPSTST AQEQDDVLIV DSDEEGPSNN ADISEEERSR KRKLDEKESV SAKRSRIEQA EELDEVIALD //