ID A3DBR2_ACET2 Unreviewed; 434 AA. AC A3DBR2; DT 20-MAR-2007, integrated into UniProtKB/TrEMBL. DT 20-MAR-2007, sequence version 1. DT 19-JAN-2022, entry version 86. DE SubName: Full=RNA modification enzyme, MiaB family {ECO:0000313|EMBL:ABN51391.1}; GN OrderedLocusNames=Cthe_0150 {ECO:0000313|EMBL:ABN51391.1}; OS Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC OS 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Hungateiclostridium OS thermocellum). OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; OC Acetivibrio. OX NCBI_TaxID=203119 {ECO:0000313|EMBL:ABN51391.1, ECO:0000313|Proteomes:UP000002145}; RN [1] {ECO:0000313|Proteomes:UP000002145} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / RC NRRL B-4536 / VPI 7372 {ECO:0000313|Proteomes:UP000002145}; RG US DOE Joint Genome Institute; RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., RA Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., RA Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D., RA Newcomb M., Richardson P.; RT "Complete sequence of Clostridium thermocellum ATCC 27405."; RL Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|EMBL:ABN51391.1, ECO:0000313|Proteomes:UP000002145} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / RC NRRL B-4536 / VPI 7372 {ECO:0000313|Proteomes:UP000002145}; RX PubMed=24295562; DOI=10.1186/1754-6834-6-179; RA Wilson C.M., Rodriguez M.Jr., Johnson C.M., Martin S.L., Chu T.M., RA Wolfinger R.D., Hauser L.J., Land M.L., Klingeman D.M., Syed M.H., RA Ragauskas A.J., Tschaplinski T.J., Mielenz J.R., Brown S.D.; RT "Global transcriptome analysis of Clostridium thermocellum ATCC 27405 RT during growth on dilute acid pretreated Populus and switchgrass."; RL Biotechnol. Biofuels 6:179-179(2013). CC -!- COFACTOR: CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; CC Evidence={ECO:0000256|ARBA:ARBA00001966}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP000568; ABN51391.1; -; Genomic_DNA. DR RefSeq; WP_003518029.1; NC_009012.1. DR STRING; 203119.Cthe_0150; -. DR EnsemblBacteria; ABN51391; ABN51391; Cthe_0150. DR KEGG; cth:Cthe_0150; -. DR eggNOG; COG0621; Bacteria. DR HOGENOM; CLU_018697_1_0_9; -. DR OMA; HFHIPLQ; -. DR OrthoDB; 397139at2; -. DR BioCyc; CTHE203119:G1G86-156-MONOMER; -. DR Proteomes; UP000002145; Chromosome. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW. DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0035598; F:N6-threonylcarbomyladenosine methylthiotransferase activity; IEA:InterPro. DR Gene3D; 3.40.50.12160; -; 1. DR Gene3D; 3.80.30.20; -; 1. DR InterPro; IPR006638; Elp3/MiaB/NifB. DR InterPro; IPR005839; Methylthiotransferase. DR InterPro; IPR020612; Methylthiotransferase_CS. DR InterPro; IPR013848; Methylthiotransferase_N. DR InterPro; IPR038135; Methylthiotransferase_N_sf. DR InterPro; IPR006467; MiaB-like_C. DR InterPro; IPR007197; rSAM. DR InterPro; IPR023404; rSAM_horseshoe. DR InterPro; IPR034557; ThrcA_tRNA_MEthiotransferase. DR InterPro; IPR002792; TRAM_dom. DR Pfam; PF04055; Radical_SAM; 1. DR Pfam; PF00919; UPF0004; 1. DR SFLD; SFLDS00029; Radical_SAM; 1. DR SFLD; SFLDF00295; threonylcarbamoyladenosine_tRN; 1. DR SMART; SM00729; Elp3; 1. DR TIGRFAMs; TIGR01579; MiaB-like-C; 1. DR TIGRFAMs; TIGR00089; TIGR00089; 1. DR PROSITE; PS51449; MTTASE_N; 1. DR PROSITE; PS01278; MTTASE_RADICAL; 1. DR PROSITE; PS51918; RADICAL_SAM; 1. DR PROSITE; PS50926; TRAM; 1. PE 4: Predicted; KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485}; KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490}; KW Iron {ECO:0000256|ARBA:ARBA00023004}; KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723}; KW Reference proteome {ECO:0000313|Proteomes:UP000002145}; KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}; KW Transferase {ECO:0000256|ARBA:ARBA00022679}. FT DOMAIN 2..114 FT /note="MTTase N-terminal" FT /evidence="ECO:0000259|PROSITE:PS51449" FT DOMAIN 139..368 FT /note="Radical SAM core" FT /evidence="ECO:0000259|PROSITE:PS51918" FT DOMAIN 371..434 FT /note="TRAM" FT /evidence="ECO:0000259|PROSITE:PS50926" SQ SEQUENCE 434 AA; 49691 MW; FB7F868991CD2383 CRC64; MKRAAFYTLG CKVNQYETEA ISEMFEKAGY KIVDFEDEAD VYVINTCTVT NLSDRKSRQM IRRAKRNNEN SIVIVIGCYA QTAPEEVSKI EGVNLVVGTK DRSRILEYLK ELETSGGRRN YVGDIMKTRE FEELGVNVYK ERTRAFIKIQ EGCNQFCTYC IIPYARGPVR SRSEENILKE VSGLAHSGYK EVVLTGIHVA SYGKDIKNTS LIDIIRKVHE IEGIERIRLG SIEPTTVTEE FVRAIKGMEK LCPQFHISLQ SGCDSTLKRM NRKYTTKEYL RSVELLRENL KDVAVTTDVM VGFPGETDEE FNETCRFVEK VLFARMHVFK YSRRKGTPAA SYPDQVAPQK KEERSRILIE LASRMTLEYN KSFTGRVLPV LFEQEVKGKE GFMEGLTPNY IRVECKGDKD IEGQILNVLL REAKDDYIVG EIVN //