ID A2PZ70_CRYJA Unreviewed; 316 AA. AC A2PZ70; DT 06-MAR-2007, integrated into UniProtKB/TrEMBL. DT 06-MAR-2007, sequence version 1. DT 12-AUG-2020, entry version 48. DE RecName: Full=S-adenosylmethionine synthase {ECO:0000256|RuleBase:RU000541}; DE EC=2.5.1.6 {ECO:0000256|RuleBase:RU000541}; DE Flags: Fragment; GN Name=CC2188 {ECO:0000313|EMBL:BAF46024.1}; OS Cryptomeria japonica (Japanese cedar) (Cupressus japonica). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Pinopsida; Pinidae; Cupressales; Cupressaceae; Cryptomeria. OX NCBI_TaxID=3369 {ECO:0000313|EMBL:BAF46024.1}; RN [1] {ECO:0000313|EMBL:BAF46024.1} RP NUCLEOTIDE SEQUENCE. RA Kado T., Matsumoto A., Ujino-Ihara T., Tsumura Y.; RT "Amounts and patterns of nucleotide variation within and between two RT Japanese conifers, sugi (Cryptomeria japonica) and hinoki (Chamaecyparis RT obtusa) (Cupressaceae sensu lato)."; RL Tree Genet. Genomes 4:133-141(2008). CC -!- FUNCTION: Catalyzes the formation of S-adenosylmethionine from CC methionine and ATP. {ECO:0000256|RuleBase:RU000541}. CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + H2O + L-methionine = diphosphate + phosphate + S- CC adenosyl-L-methionine; Xref=Rhea:RHEA:21080, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:43474, CC ChEBI:CHEBI:57844, ChEBI:CHEBI:59789; EC=2.5.1.6; CC Evidence={ECO:0000256|ARBA:ARBA00001513, CC ECO:0000256|RuleBase:RU000541}; CC -!- COFACTOR: CC Name=Co(2+); Xref=ChEBI:CHEBI:48828; CC Evidence={ECO:0000256|ARBA:ARBA00001941}; CC -!- COFACTOR: CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; CC Evidence={ECO:0000256|ARBA:ARBA00001946}; CC -!- PATHWAY: Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; CC S-adenosyl-L-methionine from L-methionine: step 1/1. CC {ECO:0000256|ARBA:ARBA00005224, ECO:0000256|RuleBase:RU000541}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. CC -!- SIMILARITY: Belongs to the AdoMet synthase family. CC {ECO:0000256|ARBA:ARBA00009685, ECO:0000256|RuleBase:RU004462}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AB264254; BAF46024.1; -; Genomic_DNA. DR UniPathway; UPA00315; UER00080. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0004478; F:methionine adenosyltransferase activity; IEA:UniProtKB-EC. DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW. DR GO; GO:0006556; P:S-adenosylmethionine biosynthetic process; IEA:UniProtKB-UniPathway. DR InterPro; IPR022631; ADOMET_SYNTHASE_CS. DR InterPro; IPR022630; S-AdoMet_synt_C. DR InterPro; IPR022629; S-AdoMet_synt_central. DR InterPro; IPR002133; S-AdoMet_synthetase. DR InterPro; IPR022636; S-AdoMet_synthetase_sfam. DR PANTHER; PTHR11964; PTHR11964; 1. DR Pfam; PF02773; S-AdoMet_synt_C; 1. DR Pfam; PF02772; S-AdoMet_synt_M; 1. DR SUPFAM; SSF55973; SSF55973; 3. DR TIGRFAMs; TIGR01034; metK; 1. DR PROSITE; PS00376; ADOMET_SYNTHASE_1; 1. DR PROSITE; PS00377; ADOMET_SYNTHASE_2; 1. PE 3: Inferred from homology; KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU000541}; KW Cobalt {ECO:0000256|ARBA:ARBA00023285}; KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490}; KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|RuleBase:RU000541}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, KW ECO:0000256|RuleBase:RU000541}; KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, KW ECO:0000256|RuleBase:RU000541}; KW One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563, KW ECO:0000256|RuleBase:RU000541}; KW Potassium {ECO:0000256|ARBA:ARBA00022958, ECO:0000256|RuleBase:RU000541}; KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU000541}. FT DOMAIN 50..170 FT /note="S-AdoMet_synt_M" FT /evidence="ECO:0000259|Pfam:PF02772" FT DOMAIN 172..313 FT /note="S-AdoMet_synt_C" FT /evidence="ECO:0000259|Pfam:PF02773" FT NON_TER 1 FT /evidence="ECO:0000313|EMBL:BAF46024.1" FT NON_TER 316 FT /evidence="ECO:0000313|EMBL:BAF46024.1" SQ SEQUENCE 316 AA; 34426 MW; 7D4A34726149791F CRC64; VRKTCREIGF ISDDVGLDAD KCRVLVNIEQ QSPDIAQGVH GHLTKRPEDI GAGDQGHMFG YATDETPELM PLTHVLATKL GAKLTDVRKN GTCPWLRPDG KTQVTIEYKN EKGAMVPLRV HTVLISTQHD ETVTNDQIAA DLKEHVIKPV IPAKYMDENT IFHLNPSGRF VIGGPHGDAG LTGRKIIIDT YGGWGAHGGG AFSGKDPTKV DRSGAYIVRQ AAKSIVAAGL ARRCIVQVSY AIGVPEPLSV FVDSYGTGKI PDKEILNIIK TSFDFRPGMI SINLDLKRGG NGRFQKTAAY GHFGRDDPDF TWEIVK //