ID A1R7L9_ARTAT Unreviewed; 365 AA. AC A1R7L9; DT 06-FEB-2007, integrated into UniProtKB/TrEMBL. DT 06-FEB-2007, sequence version 1. DT 03-OCT-2012, entry version 45. DE RecName: Full=Ketol-acid reductoisomerase; DE EC=1.1.1.86; DE AltName: Full=Acetohydroxy-acid isomeroreductase; DE AltName: Full=Alpha-keto-beta-hydroxylacil reductoisomerase; GN Name=ilvC; OrderedLocusNames=AAur_2507; OS Arthrobacter aurescens (strain TC1). OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Micrococcineae; Micrococcaceae; Arthrobacter. OX NCBI_TaxID=290340; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=TC1; RX PubMed=17194220; DOI=10.1371/journal.pgen.0020214; RA Mongodin E.F., Shapir N., Daugherty S.C., DeBoy R.T., Emerson J.B., RA Shvartzbeyn A., Radune D., Vamathevan J., Riggs F., Grinberg V., RA Khouri H.M., Wackett L.P., Nelson K.E., Sadowsky M.J.; RT "Secrets of soil survival revealed by the genome sequence of RT Arthrobacter aurescens TC1."; RL PLoS Genet. 2:2094-2106(2006). CC -!- CATALYTIC ACTIVITY: (2R,3R)-2,3-dihydroxy-3-methylpentanoate + CC NADP(+) = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. CC -!- CATALYTIC ACTIVITY: (R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) CC = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. CC -!- PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; L- CC isoleucine from 2-oxobutanoate: step 2/4. CC -!- PATHWAY: Amino-acid biosynthesis; L-valine biosynthesis; L-valine CC from pyruvate: step 2/4. CC -!- SIMILARITY: Belongs to the ketol-acid reductoisomerase family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP000474; ABM08654.1; -; Genomic_DNA. DR RefSeq; YP_948231.1; NC_008711.1. DR ProteinModelPortal; A1R7L9; -. DR SMR; A1R7L9; 27-352. DR STRING; A1R7L9; -. DR GeneID; 4640164; -. DR GenomeReviews; CP000474_GR; AAur_2507. DR KEGG; aau:AAur_2507; -. DR PATRIC; 20980450; VBIArtAur67810_2540. DR eggNOG; COG0059; -. DR HOGENOM; HOG000016230; -. DR KO; K00053; -. DR OMA; MAYAKGI; -. DR ProtClustDB; PRK05479; -. DR UniPathway; UPA00047; UER00056. DR UniPathway; UPA00049; UER00060. DR GO; GO:0050662; F:coenzyme binding; IEA:InterPro. DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW. DR GO; GO:0004455; F:ketol-acid reductoisomerase activity; IEA:HAMAP. DR GO; GO:0009097; P:isoleucine biosynthetic process; IEA:HAMAP. DR GO; GO:0009099; P:valine biosynthetic process; IEA:HAMAP. DR Gene3D; G3DSA:1.10.1040.10; Opine_DH; 1. DR HAMAP; MF_00435; IlvC; 1; -. DR InterPro; IPR008927; 6-PGluconate_DH_C-like. DR InterPro; IPR013023; AcH_isomrdctse. DR InterPro; IPR000506; AcH_isomrdctse_C. DR InterPro; IPR013328; DH_multihelical. DR InterPro; IPR013116; IlvN. DR PANTHER; PTHR21371; PTHR21371; 1. DR Pfam; PF01450; IlvC; 1. DR Pfam; PF07991; IlvN; 1. DR SUPFAM; SSF48179; 6DGDH_C_like; 1. DR TIGRFAMs; TIGR00465; ilvC; 1. PE 3: Inferred from homology; KW Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; KW Complete proteome; Isomerase; NADP; Oxidoreductase. FT ACT_SITE 132 132 By similarity. SQ SEQUENCE 365 AA; 39660 MW; B9650A5A2BCE9527 CRC64; MSLAPSTAAP LSISTKNHSR GVTQVTEMFY DDDADLSIIQ GRKVAIVGYG SQGHAHALNL RDSGVEVVIA LKDGSKSTSK AEDAGFTVKN VADAAEWADV IMILAPDQHQ RSIYNDSIKD KLTEGKALAF AHGFNIRFGY IEAPAGVDVI LIAPKAPGHT VRREFEAGRG IPDIIAVEQD ASGSAWDLAK SYAKAIGGTR AGVIKTTFTE ETETDLFGEQ AVLCGGVSQL VQYGFETLTE AGYQPQIAYF EVLHELKLIV DLMWEGGIAK QRWSVSDTAE YGDYVSGPRV ITPEVKENMK AVLADIQNGA FAKRFIDDQD NGGTEFKELR AKAEQHPIEE VGRELRSLFS WQQQDADYVE GSAAR //