ID HOG1_BOTFB Reviewed; 354 AA.
AC A1IVT7; A6RRX4;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 1.
DT 03-MAR-2009, entry version 18.
DE RecName: Full=Mitogen-activated protein kinase hog1;
DE Short=MAP kinase hog1;
DE EC=2.7.11.24;
DE AltName: Full=Stress-activated mitogen-activated protein kinase;
DE AltName: Full=BcSAK1;
GN Name=hog1; Synonyms=sak1; ORFNames=BC1G_03001;
OS Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis
OS cinerea).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Helotiales; Sclerotiniaceae; Botryotinia.
OX NCBI_TaxID=332648;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND PHOSPHORYLATION.
RX PubMed=17189492; DOI=10.1128/EC.00153-06;
RA Segmueller N., Ellendorf U., Tudzynski B., Tudzynski P.;
RT "BcSAK1, a stress-activated mitogen-activated protein kinase, is
RT involved in vegetative differentiation and pathogenicity in Botrytis
RT cinerea.";
RL Eukaryot. Cell 6:211-221(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Birren B.W., Galagan J.E., Lander E.S., Devon K., Nusbaum C.,
RA Cuomo C., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M.,
RA Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K.,
RA LaButti K., Pushparaj V., DeCaprio D., Crawford M., Koehrsen M.,
RA Engels R., Montgomery P., Pearson M., Howarth C., Alvarado L.,
RA Kodira C.D., Zeng Q., Yandava C., O'Leary S., Amedeo P., Briggs S.,
RA Baker S., Goff S., Hohn T., Hutchison D., Lam S., Martin C.,
RA Miguel T., Rose M., Turgeon B.G., Wu G., Yoder O., Zheng L.;
RT "Annotation of the Botryotinia fuckeliana B05.10 genome.";
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Mitogen-activated protein kinase involved in a signal
CC transduction pathway that is activated by changes in the
CC osmolarity of the extracellular environment. Controls osmotic
CC regulation of transcription of target genes (By similarity).
CC Involved in vegetative and pathogenic development like conidia
CC formation, sclerotial development, and in penetration into
CC unwounded plant tissue.
CC -!- CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
CC -!- COFACTOR: Magnesium (By similarity).
CC -!- ENZYME REGULATION: Activated by tyrosine and threonine
CC phosphorylation (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By
CC similarity).
CC -!- DOMAIN: The TXY motif contains the threonine and tyrosine residues
CC whose phosphorylation activates the MAP kinases.
CC -!- PTM: Dually phosphorylated on Thr-171 and Tyr-173, which activates
CC the enzyme (By similarity). Phosphorylated under osmotic stress,
CC specific fungicides, and oxidative stress mediated by H(2)O(2) and
CC menadione.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr
CC protein kinase family. MAP kinase subfamily. HOG1 sub-subfamily.
CC -!- SIMILARITY: Contains 1 protein kinase domain.
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DR EMBL; AM236311; CAJ85638.1; -; Genomic_DNA.
DR EMBL; CH476852; EDN18852.1; -; Genomic_DNA.
DR RefSeq; XP_001558337.1; -.
DR GeneID; 5438947; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0004707; F:MAP kinase activity; IEA:InterPro.
DR GO; GO:0006468; P:protein amino acid phosphorylation; IEA:InterPro.
DR GO; GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
DR InterPro; IPR003527; MAP_kinase_CS.
DR InterPro; IPR008352; MAPK_p38.
DR InterPro; IPR000719; Prot_kinase_core.
DR InterPro; IPR017441; Protein_kinase_ATP_bd_CS.
DR InterPro; IPR017442; Se/Thr_pkinase-rel.
DR InterPro; IPR008271; Ser_thr_pkin_AS.
DR InterPro; IPR002290; Ser_thr_pkinase.
DR Pfam; PF00069; Pkinase; 1.
DR PRINTS; PR01773; P38MAPKINASE.
DR ProDom; PD000001; Prot_kinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR PROSITE; PS01351; MAPK; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 1: Evidence at protein level;
KW Activator; ATP-binding; Complete proteome; Cytoplasm; Kinase;
KW Nucleotide-binding; Nucleus; Phosphoprotein;
KW Serine/threonine-protein kinase; Transcription;
KW Transcription regulation; Transferase.
FT CHAIN 1 354 Mitogen-activated protein kinase hog1.
FT /FTId=PRO_0000289679.
FT DOMAIN 20 299 Protein kinase.
FT NP_BIND 26 34 ATP (By similarity).
FT MOTIF 171 173 TXY.
FT ACT_SITE 141 141 Proton acceptor (By similarity).
FT BINDING 49 49 ATP (By similarity).
FT MOD_RES 171 171 Phosphothreonine (By similarity).
FT MOD_RES 173 173 Phosphotyrosine (By similarity).
SQ SEQUENCE 354 AA; 40499 MW; 4C0DB95CE772A627 CRC64;
MAEFVRAQIF GTTFEITSRY SDLQPVGMGA FGLVCSAKDN LTGSNVAVKK IMKPFSTPVL
SKRTYRELKL LKHLKHENVI SLSDIFISPL EDIYFVTELL GTDLHRLLTS RPLEKQFIQY
FLYQILRGLK YVHSAGVVHR DLKPSNILVN ENCDLKICDF GLARIQDPQM TGYVSTRYYR
APEIMLTWQK YDVEVDVWSA GCIFAEMLEG KPLFPGKDHV NQFSIITELL GTPPDDVIHT
IASENTLRFV QSLPKRERQP LASKFTQADP LAIDLLEKML VFDPRARIKA AEGLAHEYLS
PYHDPTDEPA AEERFDWSFN DADLPVDTWK IMMYSEILDY HNVINDAQNL TESQ
//