ID FTMG_NEOFI Reviewed; 504 AA. AC A1DA65; DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot. DT 23-JAN-2007, sequence version 1. DT 05-JUL-2017, entry version 68. DE RecName: Full=Fumitremorgin C monooxygenase; DE EC=1.14.13.177; DE AltName: Full=Fumitremorgin biosynthesis protein G; GN Name=ftmP450-3; Synonyms=ftmG; ORFNames=NFIA_093750; OS Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC OS A1164 / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus). OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes; OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus. OX NCBI_TaxID=331117; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / RC NRRL 181 / WB 181; RX PubMed=18404212; DOI=10.1371/journal.pgen.1000046; RA Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., RA Anderson M.J., Crabtree J., Silva J.C., Badger J.H., Albarraq A., RA Angiuoli S., Bussey H., Bowyer P., Cotty P.J., Dyer P.S., Egan A., RA Galens K., Fraser-Liggett C.M., Haas B.J., Inman J.M., Kent R., RA Lemieux S., Malavazi I., Orvis J., Roemer T., Ronning C.M., RA Sundaram J.P., Sutton G., Turner G., Venter J.C., White O.R., RA Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H., Wortman J.R., RA Jiang B., Denning D.W., Nierman W.C.; RT "Genomic islands in the pathogenic filamentous fungus Aspergillus RT fumigatus."; RL PLoS Genet. 4:E1000046-E1000046(2008). CC -!- FUNCTION: Catalyzes the conversion of fumitremorgin C to 12,13- CC dihydroxyfumitremorgin C in the biosynthesis of indole alkaloid CC verruculogen. {ECO:0000250}. CC -!- CATALYTIC ACTIVITY: Fumitremorgin C + 2 NADPH + 2 O(2) = 12- CC alpha,13-alpha-dihydroxyfumitremorgin C + 2 NADP(+) + 2 H(2)O. CC -!- PATHWAY: Alkaloid biosynthesis. CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane CC protein {ECO:0000305}. CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; DS027694; EAW19755.1; -; Genomic_DNA. DR RefSeq; XP_001261652.1; XM_001261651.1. DR ProteinModelPortal; A1DA65; -. DR STRING; 36630.CADNFIAP00008576; -. DR EnsemblFungi; CADNFIAT00008790; CADNFIAP00008576; CADNFIAG00008790. DR GeneID; 4588685; -. DR KEGG; nfi:NFIA_093750; -. DR EuPathDB; FungiDB:NFIA_093750; -. DR HOGENOM; HOG000171616; -. DR KO; K17826; -. DR OrthoDB; EOG092C1JLC; -. DR Proteomes; UP000006702; Unassembled WGS sequence. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0020037; F:heme binding; IEA:InterPro. DR GO; GO:0005506; F:iron ion binding; IEA:InterPro. DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW. DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro. DR GO; GO:0009820; P:alkaloid metabolic process; IEA:UniProtKB-KW. DR InterPro; IPR001128; Cyt_P450. DR InterPro; IPR017972; Cyt_P450_CS. DR InterPro; IPR002403; Cyt_P450_E_grp-IV. DR Pfam; PF00067; p450; 1. DR PRINTS; PR00465; EP450IV. DR PRINTS; PR00385; P450. DR SUPFAM; SSF48264; SSF48264; 1. DR PROSITE; PS00086; CYTOCHROME_P450; 1. PE 3: Inferred from homology; KW Alkaloid metabolism; Complete proteome; Heme; Iron; Membrane; KW Metal-binding; Monooxygenase; NADP; Oxidoreductase; KW Reference proteome; Transmembrane; Transmembrane helix. FT CHAIN 1 504 Fumitremorgin C monooxygenase. FT /FTId=PRO_0000424126. FT TRANSMEM 9 29 Helical. {ECO:0000255}. FT METAL 442 442 Iron (heme axial ligand). {ECO:0000250}. SQ SEQUENCE 504 AA; 57231 MW; 8797F1A7339B8999 CRC64; METFDAIQLP YPGVVGASLL VILGIILLFP LDTGHFISIN QHPWDFFQTK AKQEFEYNAA ALLNEGLQTG RSAFRLVTNM VTYLILKDQY AEEIKNDSRF GAHEAVDPVL LVDLPGLETM FQGSLHNQVP PMAVRALNKE LVHLTPFLSE EAMNCLQTRW TDSAEWHDVS IPETVLALIA QMTTRALLGP ALCRNPEWLD IAKSFTTNRA IAVAAVQSWP SFLQPVIHWF LSPCRALRRQ IQCARNILLP VLERERRSYR SDQPTKREFS NLAFIDQYAK GARYDATMAQ LRIIAVAFQT TSDLVEKVIA RLCKHPELIQ PLREEVVSVV GKNGLHSHSL RKLTLMESVM KETQRLEPAV IIGMFRLAKE KVTLKDGTVI PKGTNIAFAN DLRFDPEMYP EPETFDGYRF QRMREDPEKI DLTPFTKTRM SHLAFGHGKH ACPGRFLACD EAKLILCHIL LKYEIRAVEG SPPELRARGM FVQLDPGAMM SVRRRRENEF ALHG //