ID ACPS_MYCS2 Reviewed; 130 AA. AC A0R1H6; DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot. DT 09-JAN-2007, sequence version 1. DT 10-AUG-2010, entry version 34. DE RecName: Full=Holo-[acyl-carrier-protein] synthase; DE Short=Holo-ACP synthase; DE EC=2.7.8.7; DE AltName: Full=4'-phosphopantetheinyl transferase AcpS; GN Name=acpS; OrderedLocusNames=MSMEG_4756; OS Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155). OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Corynebacterineae; Mycobacteriaceae; Mycobacterium. OX NCBI_TaxID=246196; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., RA Fraser C.M.; RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases. RN [2] RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], AND RP CLEAVAGE OF INITIATOR METHIONINE. RX PubMed=18955433; DOI=10.1101/gr.081901.108; RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.; RT "Ortho-proteogenomics: multiple proteomes investigation through RT orthology and a new MS-based protocol."; RL Genome Res. 19:128-135(2009). CC -!- FUNCTION: Transfers the 4'-phosphopantetheine moiety from coenzyme CC A to a Ser of acyl-carrier-protein (By similarity). CC -!- CATALYTIC ACTIVITY: CoA-(4'-phosphopantetheine) + apo-[acyl- CC carrier-protein] = adenosine 3',5'-bisphosphate + holo-[acyl- CC carrier-protein]. CC -!- COFACTOR: Magnesium (By similarity). CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity). CC -!- SIMILARITY: Belongs to the P-Pant transferase superfamily. AcpS CC family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP000480; ABK75003.1; -; Genomic_DNA. DR RefSeq; YP_889014.1; -. DR PDB; 3GWM; X-ray; 1.70 A; A=2-130. DR PDBsum; 3GWM; -. DR ProteinModelPortal; A0R1H6; -. DR SMR; A0R1H6; 1-128. DR STRING; A0R1H6; -. DR EnsemblBacteria; EBMYCT00000046152; EBMYCP00000044489; EBMYCG00000046147. DR GeneID; 4536674; -. DR GenomeReviews; CP000480_GR; MSMEG_4756. DR KEGG; msm:MSMEG_4756; -. DR NMPDR; fig|246196.1.peg.4746; -. DR TIGR; MSMEG_4756; -. DR eggNOG; COG0736; -. DR HOGENOM; HBG734479; -. DR OMA; IKAWSQA; -. DR ProtClustDB; PRK00070; -. DR BioCyc; MSME246196:MSMEG_4756-MONOMER; -. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0008897; F:holo-[acyl-carrier-protein] synthase activity; IEA:EC. DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro. DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW. DR GO; GO:0009059; P:macromolecule biosynthetic process; IEA:InterPro. DR HAMAP; MF_00101; AcpS; 1; -. DR InterPro; IPR008278; 4-PPantetheinyl_Trfase. DR InterPro; IPR002582; ACPS. DR InterPro; IPR004568; PPantethiene-prot_Trfase. DR Pfam; PF01648; ACPS; 1. DR ProDom; PD004282; PPantethiene-prot_Trfase; 1. DR SUPFAM; SSF56214; 4-PPT_transf; 1. DR TIGRFAMs; TIGR00516; acpS; 1. DR TIGRFAMs; TIGR00556; pantethn_trn; 1. PE 1: Evidence at protein level; KW 3D-structure; Complete proteome; Cytoplasm; Fatty acid biosynthesis; KW Lipid synthesis; Magnesium; Metal-binding; Transferase. FT INIT_MET 1 1 Removed. FT CHAIN 2 130 Holo-[acyl-carrier-protein] synthase. FT /FTId=PRO_1000008454. FT METAL 9 9 Magnesium (By similarity). FT METAL 58 58 Magnesium (By similarity). SQ SEQUENCE 130 AA; 14165 MW; 574FE43BAA348A94 CRC64; MAIVGVGIDL VSIPDFAEQV DRPGTVFAET FTPGERRDAA DKSSSAARHL AARWAAKEAV IKAWSSSRFS KRPALPEGIH RDIEVVTDMW GRPKVRLSGE IAKHLEDVTI HVSLTHEDQT AAAVAIIEEP //