ID ECCA1_MYCS2 Reviewed; 574 AA. AC A0QNI9; I7FVG3; DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot. DT 09-JAN-2007, sequence version 1. DT 24-JAN-2024, entry version 103. DE RecName: Full=ESX-1 secretion system protein EccA1 {ECO:0000303|PubMed:19876390}; DE AltName: Full=ESX conserved component A1 {ECO:0000303|PubMed:19876390}; DE AltName: Full=Type VII secretion system protein EccA1 {ECO:0000305}; DE Short=T7SS protein EccA1 {ECO:0000305}; GN Name=eccA1 {ECO:0000303|PubMed:19876390}; GN Synonyms=Ms3868 {ECO:0000303|PubMed:15314236}, GN Sm3868 {ECO:0000303|PubMed:15687187}; GN OrderedLocusNames=MSMEG_0059, MSMEI_0060; OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium OS smegmatis). OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae; OC Mycolicibacterium. OX NCBI_TaxID=246196; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 700084 / mc(2)155; RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., RA Fraser C.M.; RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases. RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 700084 / mc(2)155; RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20; RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.; RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic RT mutations or sequencing errors?"; RL Genome Biol. 8:R20.1-R20.9(2007). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 700084 / mc(2)155; RX PubMed=18955433; DOI=10.1101/gr.081901.108; RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.; RT "Ortho-proteogenomics: multiple proteomes investigation through orthology RT and a new MS-based protocol."; RL Genome Res. 19:128-135(2009). RN [4] RP FUNCTION, AND DISRUPTION PHENOTYPE. RC STRAIN=ATCC 700084 / mc(2)155; RX PubMed=15314236; DOI=10.1073/pnas.0404892101; RA Flint J.L., Kowalski J.C., Karnati P.K., Derbyshire K.M.; RT "The RD1 virulence locus of Mycobacterium tuberculosis regulates DNA RT transfer in Mycobacterium smegmatis."; RL Proc. Natl. Acad. Sci. U.S.A. 101:12598-12603(2004). RN [5] RP DISRUPTION PHENOTYPE. RC STRAIN=ATCC 700084 / mc(2)155; RX PubMed=15687187; DOI=10.1128/jb.187.4.1238-1245.2005; RA Converse S.E., Cox J.S.; RT "A protein secretion pathway critical for Mycobacterium tuberculosis RT virulence is conserved and functional in Mycobacterium smegmatis."; RL J. Bacteriol. 187:1238-1245(2005). RN [6] RP FUNCTION, AND DISRUPTION PHENOTYPE. RC STRAIN=MKD8; RX PubMed=18554329; DOI=10.1111/j.1365-2958.2008.06299.x; RA Coros A., Callahan B., Battaglioli E., Derbyshire K.M.; RT "The specialized secretory apparatus ESX-1 is essential for DNA transfer in RT Mycobacterium smegmatis."; RL Mol. Microbiol. 69:794-808(2008). RN [7] RP NOMENCLATURE. RX PubMed=19876390; DOI=10.1371/journal.ppat.1000507; RA Bitter W., Houben E.N., Bottai D., Brodin P., Brown E.J., Cox J.S., RA Derbyshire K., Fortune S.M., Gao L.Y., Liu J., Gey van Pittius N.C., RA Pym A.S., Rubin E.J., Sherman D.R., Cole S.T., Brosch R.; RT "Systematic genetic nomenclature for type VII secretion systems."; RL PLoS Pathog. 5:E1000507-E1000507(2009). CC -!- FUNCTION: Part of the ESX-1 / type VII specialized secretion system CC (T7SS), which exports several proteins including EsxA and EsxB CC (PubMed:15687187). Plays a role in DNA conjugation, in both donor and CC recipient strains (PubMed:15314236, PubMed:18554329). EccA1 exhibits CC ATPase activity and may provide energy for the export of ESX-1 CC substrates (By similarity). {ECO:0000250|UniProtKB:P9WPH9, CC ECO:0000269|PubMed:15314236, ECO:0000269|PubMed:18554329}. CC -!- SUBUNIT: Part of the ESX-1 / type VII secretion system (T7SS), which is CC composed of cytosolic and membrane components (By similarity). CC {ECO:0000250|UniProtKB:P9WPH9, ECO:0000305|PubMed:19876390}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:B2HSU9}. CC -!- DISRUPTION PHENOTYPE: Increases efficiency of DNA conjugation when CC disrupted in donor strain (PubMed:15314236). Loss of DNA conjugation CC when disrupted in recipient strain (PubMed:18554329) No effect on CC secretion of EsxA or EsxB (PubMed:15687187). EsxB secretion was not CC seen in this disruption in strain MDK8 (PubMed:18554329). CC {ECO:0000269|PubMed:15314236, ECO:0000269|PubMed:15687187, CC ECO:0000269|PubMed:18554329}. CC -!- MISCELLANEOUS: DNA conjugation in M.smegmatis is unidirectional with CC distinct donor and recipient strains; mc(2)155 is a donor strain while CC MKD8 is a recipient strain. Mutations in a donor strain that alter DNA CC transfer do not always alter DNA transfer in a recipient strain. CC {ECO:0000305|PubMed:15314236}. CC -!- SIMILARITY: Belongs to the CbxX/CfxQ family. {ECO:0000305}. CC -!- SEQUENCE CAUTION: CC Sequence=AFP36542.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP000480; ABK74506.1; -; Genomic_DNA. DR EMBL; CP001663; AFP36542.1; ALT_INIT; Genomic_DNA. DR RefSeq; WP_003891386.1; NZ_SIJM01000058.1. DR RefSeq; YP_884477.1; NC_008596.1. DR AlphaFoldDB; A0QNI9; -. DR SMR; A0QNI9; -. DR STRING; 246196.MSMEG_0059; -. DR TCDB; 3.A.24.5.1; the type vii or esx protein secretion system (t7ss) family. DR PaxDb; 246196-MSMEI_0060; -. DR GeneID; 66738249; -. DR KEGG; msg:MSMEI_0060; -. DR KEGG; msm:MSMEG_0059; -. DR PATRIC; fig|246196.19.peg.57; -. DR eggNOG; COG0464; Bacteria. DR OMA; QASKHMI; -. DR OrthoDB; 9806903at2; -. DR Proteomes; UP000000757; Chromosome. DR Proteomes; UP000006158; Chromosome. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro. DR CDD; cd00009; AAA; 1. DR Gene3D; 1.10.8.60; -; 1. DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1. DR Gene3D; 1.25.40.10; Tetratricopeptide repeat domain; 1. DR InterPro; IPR003593; AAA+_ATPase. DR InterPro; IPR003959; ATPase_AAA_core. DR InterPro; IPR000641; CbxX/CfxQ. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR023835; T7SS_EccA. DR InterPro; IPR049078; T7SS_EccA1-like_N. DR InterPro; IPR011990; TPR-like_helical_dom_sf. DR NCBIfam; TIGR03922; T7SS_EccA; 1. DR PANTHER; PTHR43392; AAA-TYPE ATPASE FAMILY PROTEIN / ANKYRIN REPEAT FAMILY PROTEIN; 1. DR PANTHER; PTHR43392:SF2; AAA-TYPE ATPASE FAMILY PROTEIN / ANKYRIN REPEAT FAMILY PROTEIN; 1. DR Pfam; PF00004; AAA; 1. DR Pfam; PF21545; T7SS_EccA1_N; 1. DR PRINTS; PR00819; CBXCFQXSUPER. DR SMART; SM00382; AAA; 1. DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1. PE 3: Inferred from homology; KW ATP-binding; Cytoplasm; Nucleotide-binding; Reference proteome; Transport. FT CHAIN 1..574 FT /note="ESX-1 secretion system protein EccA1" FT /id="PRO_0000438310" FT BINDING 335..342 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT /evidence="ECO:0000305" SQ SEQUENCE 574 AA; 62148 MW; 20482F23E84D753D CRC64; MVNHLAGMFG SAVGMMSNSR ARALEIFTEI TNYDESACDA WVGRIGCGDT DRVTLFRAWY SRSNFGQLAG AAEISMNGVG ARIPIGGIYS RDITYPINSP LAITMGFAVH EASEGNYTDA MEALEDAPAA GSEHLVSWVR AVIYGAGQRW TEVIDQVRGA ERWPDKFLAA AAGVAHGVAA ANLGLFTEAD RRLTEANDTP VAQACAPVIA WYLAMARRSQ GNEESAQVLL EWLQANFPEP KVTAALRDPA YRLETTTAEK IAARSDPWDP SSVVADTSAR DKLLVEAQAE LDRQIGLGRV KEQIEAYRAA TQMARIRAAR GMKVAQTSKH MIFAGPPGTG KTTIARVVAN ILAGLGVIAE PKLIETSRKD FVAEYEGQSA VKTSKTIDRA LGGVLFIDEA YTLVQERNGQ TDPFGAEALD TLLARMENDR DRLVVIIAGY SNDIDRLLEV NDGLRSRFAT RIEFDSYSPD EIVEISKVIA TANDSRLDDT AAKRVLEAAT TLSQRSLNGK PALDIAGNGR YARQLVEAGE QNRDMRLARS LDFDSLGVDQ LSEINGDDMA AAIASVHSRL NIGE //