ID A0AAN0V819_9PSED Unreviewed; 759 AA. AC A0AAN0V819; DT 02-OCT-2024, integrated into UniProtKB/TrEMBL. DT 02-OCT-2024, sequence version 1. DT 27-NOV-2024, entry version 2. DE RecName: Full=Cyclic di-GMP-binding protein {ECO:0000256|ARBA:ARBA00021844, ECO:0000256|RuleBase:RU365021}; DE AltName: Full=Cellulose synthase regulatory subunit {ECO:0000256|ARBA:ARBA00033444, ECO:0000256|RuleBase:RU365021}; GN ORFNames=PVLB_00975 {ECO:0000313|EMBL:AGZ33005.1}; OS Pseudomonas sp. VLB120. OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. OX NCBI_TaxID=69328 {ECO:0000313|EMBL:AGZ33005.1, ECO:0000313|Proteomes:UP000017756}; RN [1] {ECO:0000313|EMBL:AGZ33005.1, ECO:0000313|Proteomes:UP000017756} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=VLB120 {ECO:0000313|EMBL:AGZ33005.1, RC ECO:0000313|Proteomes:UP000017756}; RX PubMed=25128338; DOI=10.1128/AEM.01940-14; RA Volmer J., Neumann C., Buhler B., Schmid A.; RT "Engineering of Pseudomonas taiwanensis VLB120 for Constitutive Solvent RT Tolerance and Increased Specific Styrene Epoxidation Activity."; RL Appl. Environ. Microbiol. 80:6539-6548(2014). CC -!- FUNCTION: Binds the cellulose synthase activator, bis-(3'-5') cyclic CC diguanylic acid (c-di-GMP). {ECO:0000256|ARBA:ARBA00002057, CC ECO:0000256|RuleBase:RU365021}. CC -!- PATHWAY: Glycan metabolism; bacterial cellulose biosynthesis. CC {ECO:0000256|ARBA:ARBA00005186, ECO:0000256|RuleBase:RU365021}. CC -!- SUBUNIT: Tightly associated with the cellulose synthase catalytic CC subunit. {ECO:0000256|ARBA:ARBA00011437, CC ECO:0000256|RuleBase:RU365021}. CC -!- SUBCELLULAR LOCATION: Cell inner membrane CC {ECO:0000256|ARBA:ARBA00004377}; Single-pass membrane protein CC {ECO:0000256|ARBA:ARBA00004377}. CC -!- SIMILARITY: Belongs to the AcsB/BcsB family. CC {ECO:0000256|ARBA:ARBA00010714, ECO:0000256|RuleBase:RU365021}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP003961; AGZ33005.1; -; Genomic_DNA. DR Proteomes; UP000017756; Chromosome. DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 2. DR InterPro; IPR003920; Cell_synth_B. DR InterPro; IPR018513; Cell_synthase_bac. DR PANTHER; PTHR39083; CYCLIC DI-GMP-BINDING PROTEIN; 1. DR PANTHER; PTHR39083:SF1; CYCLIC DI-GMP-BINDING PROTEIN; 1. DR Pfam; PF03170; BcsB; 1. DR PRINTS; PR01440; CELLSNTHASEB. PE 3: Inferred from homology; KW c-di-GMP {ECO:0000256|ARBA:ARBA00022636, ECO:0000256|RuleBase:RU365021}; KW Cell inner membrane {ECO:0000256|ARBA:ARBA00022519, KW ECO:0000256|RuleBase:RU365021}; KW Cell membrane {ECO:0000256|ARBA:ARBA00022475, KW ECO:0000256|RuleBase:RU365021}; KW Cellulose biosynthesis {ECO:0000256|ARBA:ARBA00022916, KW ECO:0000256|RuleBase:RU365021}; KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU365021}; KW Signal {ECO:0000256|RuleBase:RU365021}; KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, KW ECO:0000256|RuleBase:RU365021}; KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, KW ECO:0000256|RuleBase:RU365021}. FT SIGNAL 1..23 FT /evidence="ECO:0000256|RuleBase:RU365021" FT CHAIN 24..759 FT /note="Cyclic di-GMP-binding protein" FT /evidence="ECO:0000256|RuleBase:RU365021" FT /id="PRO_5042664713" FT TRANSMEM 724..744 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU365021" SQ SEQUENCE 759 AA; 84021 MW; 3E119FDB9B667ADF CRC64; MTRYPARTWG LALALLASLF QNAAVASVAP LVMPAEPTVP NWPVIKTFEQ LGQASDSLLL GVRNSENIEF GVRRDRLATE AVLQLDYTPS PALLPNLSHL RVYLNDELMG VVPVEKDQLG QRVRRQLPLD PKLVTDFNRV RLEFVGHYTD ICEDPAHSGL WLNLNRKSQV QLREQALAVE NDLAYFPLPF FDARDTSKVE LPVVFADSPT LGEQRAAAIL SSYFGSKAGW RKATFPVSFD SLPASGDKPK PAIVFASNER RPAFLADLEK FPPVDGPVVQ LIDHPTDRWS KLLLVLGRND DDLVKAASAL AVGTELFRGS RVKVEQMAAL EPRKPYDAPN WTRTDRPVRF SELLDYPEQL QVSGLQPRPI TLDVNLPPDL FVWRNQGIPL QTLYRYTPPA VTDESRLSIS VNDQYITSMP LVGNDRRSST LEEMRLSVLS TDSTALTEQS LVPALKIGAR NRLRFDFSFA STLGSAQRDH CQTSLPVDVR AAIDENSTID LSGYHHYMAM PDLRAFARSG FPYSRMADLS ETVIVVPSTP TPMQVGTLLD VVGSLAGQIG YPALNVRLSD NWRNVADADA DLLLIGTLPE SLHDVPNLGL LLNAQRDWMV QGRTLDVPGS QRFDNAPVVP NSRVEVSAQA PLAAITGLKS PFHEQRSVVA LLARSDADYA LLRDVLADSG KLEAVAGSVA LVRTSGVSSQ FVGEHYFVGE LPWWLLLWFH LSDYPMLIAV IATVSVLLFA FLLWRALRWA GRRRLGEAD //